Paraburkholderia solitsugae sp. nov. and Paraburkholderia elongata sp. nov., phenolic acid-degrading bacteria isolated from forest soil and emended description of Paraburkholderia madseniana
Two bacterial strains, 1N T and 5N T , were isolated from hemlock forest soil using a soluble organic matter enrichment. Cells of 1N T (0.65×1.85 µm) and 5N T (0.6×1.85 µm) are Gram-stain-negative, aerobic, motile, non-sporulating and exist as single rods, diplobacilli or in chains of varying length...
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| Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2020-09, Vol.70 (9), p.5093-5105 |
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| creator | Wilhelm, Roland C. Cyle, K. Taylor Martinez, Carmen Enid Karasz, David C. Newman, Jeffrey D. Buckley, Daniel H. |
| description | Two bacterial strains, 1N
T
and 5N
T
, were isolated from hemlock forest soil using a soluble organic matter enrichment. Cells of 1N
T
(0.65×1.85 µm) and 5N
T
(0.6×1.85 µm) are Gram-stain-negative, aerobic, motile, non-sporulating and exist as single rods, diplobacilli or in chains of varying length. During growth in dilute media (≤0.1× tryptic soy broth; TSB), cells are primarily motile with flagella. At higher concentrations (≥0.3× TSB), cells of both strains increasingly form non-motile chains, and cells of 5N
T
elongate (0.57×~7 µm) and form especially long filaments. Optimum growth of 1N
T
and 5N
T
occurred at 25–30 °C, pH 6.5–7.0 and |
| doi_str_mv | 10.1099/ijsem.0.004387 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_osti_</sourceid><recordid>TN_cdi_osti_scitechconnect_1802954</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2435531864</sourcerecordid><originalsourceid>FETCH-LOGICAL-c339t-d96e6f26110d9163a9839876429cefd707010e9c076b1799ba4b0bd80e23d5123</originalsourceid><addsrcrecordid>eNplkUFP3DAQhSNUJCj02rPFqYcmHceJEx8RKhQJiR7K2ZrYk11DYgfbW6l_jt9G2K24cJrRzPfmafSK4iuHioNSP9xjormCCqARfXdUnPJGyrIF0Xx672t5UnxO6RFgHQCcFi-_MeKwi0_bMFmKDlkKk8tpt0FiaamYD38rht6yDyBNwW8w4zv2nS1b8qvcMDTOlpY2Ea3zGzagyXuNW69jJsvGGGY2hkgpr45u2lvQTN6uS0vJRLdkFzwL40fnGW0i79DjeXE84pToy_96Vjxc__xz9au8u7-5vbq8K40QKpdWSZJjLTkHq7gUqHqh-k42tTI02g464EDKQCcH3ik1YDPAYHugWtiW1-KsuDjcDSk7nYzLZLYmeE8ma95Drdpmhb4doCWG5936mZ5dMjRN6Cnskq4b0baC9_INrQ6oiSGlSKNeopsx_tMc9Fuaep-mBn1IU7wC8fuXQw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2435531864</pqid></control><display><type>article</type><title>Paraburkholderia solitsugae sp. nov. and Paraburkholderia elongata sp. nov., phenolic acid-degrading bacteria isolated from forest soil and emended description of Paraburkholderia madseniana</title><source>Microbiology Society</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Wilhelm, Roland C. ; Cyle, K. Taylor ; Martinez, Carmen Enid ; Karasz, David C. ; Newman, Jeffrey D. ; Buckley, Daniel H.</creator><creatorcontrib>Wilhelm, Roland C. ; Cyle, K. Taylor ; Martinez, Carmen Enid ; Karasz, David C. ; Newman, Jeffrey D. ; Buckley, Daniel H. ; Cornell Univ., Ithaca, NY (United States)</creatorcontrib><description>Two bacterial strains, 1N
T
and 5N
T
, were isolated from hemlock forest soil using a soluble organic matter enrichment. Cells of 1N
T
(0.65×1.85 µm) and 5N
T
(0.6×1.85 µm) are Gram-stain-negative, aerobic, motile, non-sporulating and exist as single rods, diplobacilli or in chains of varying length. During growth in dilute media (≤0.1× tryptic soy broth; TSB), cells are primarily motile with flagella. At higher concentrations (≥0.3× TSB), cells of both strains increasingly form non-motile chains, and cells of 5N
T
elongate (0.57×~7 µm) and form especially long filaments. Optimum growth of 1N
T
and 5N
T
occurred at 25–30 °C, pH 6.5–7.0 and <0.5% salinity. Results of comparative chemotaxonomic, genomic and phylogenetic analyses revealed that 1N
T
and 5N
T
were distinct from one another and their closest related type strains:
Paraburkholderia madseniana
RP11
T
,
Paraburkholderia aspalathi
LMG 27731
T
and
Paraburkholderia caffeinilytica
CF1
T
. The genomes of 1N
T
and 5N
T
had an average nucleotide identity (91.6 and 91.3%) and
in silico
DNA–DNA hybridization values (45.8%±2.6 and 45.5%±2.5) and differed in functional gene content from their closest related type strains. The composition of fatty acids and patterns of substrate use, including the catabolism of phenolic acids, also differentiated strains 1N
T
and 5N
T
from each other and their closest relatives. The only ubiquinone present in strains 1N
T
and 5N
T
was Q-8. The major cellular fatty acids were C
16 : 0
, 3OH-C
16 : 0
, C
17 : 0
cyclo, C
19 : 0
cyclo ω8
c
and summed features 2 (3OH-C
14 : 0
/ C
16 : 1
iso I), 3 (C
16 : 1
ω6
c
/ω7
c
) and 8 (C
18 : 1
ω7
c
/ω6
c
). A third bacterium, strain RL16-012-BIC-B, was isolated from soil associated with shallow roots and was determined to be a strain of
P. madseniana
(ANI, 98.8%; 16S rRNA gene similarity, 100%). Characterizations of strain RL16-012-BIC-B (DSM 110723=LMG 31706) led to proposed emendments to the species description of
P. madseniana
. Our polyphasic approach demonstrated that strains 1N
T
and 5N
T
represent novel species from the genus
Paraburkholderia
for which the names
Paraburkholderia solitsugae
sp. nov. (type strain 1N
T
=DSM 110721
T
=LMG 31704
T
) and
Paraburkholderia elongata
sp. nov. (type strain 5N
T
=DSM 110722
T
=LMG 31705
T
) are proposed.</description><identifier>ISSN: 1466-5026</identifier><identifier>EISSN: 1466-5034</identifier><identifier>DOI: 10.1099/ijsem.0.004387</identifier><language>eng</language><publisher>United States: International Union of Microbiological Societies</publisher><subject>Microbiology</subject><ispartof>International journal of systematic and evolutionary microbiology, 2020-09, Vol.70 (9), p.5093-5105</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c339t-d96e6f26110d9163a9839876429cefd707010e9c076b1799ba4b0bd80e23d5123</citedby><cites>FETCH-LOGICAL-c339t-d96e6f26110d9163a9839876429cefd707010e9c076b1799ba4b0bd80e23d5123</cites><orcidid>0000-0003-0647-5341 ; 0000-0002-1824-4017 ; 0000-0003-0495-4840 ; 0000-0003-1654-2907 ; 0000-0001-8553-2118 ; 0000000306475341 ; 0000000304954840 ; 0000000218244017 ; 0000000185532118 ; 0000000316542907</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,3746,27924,27925</link.rule.ids><backlink>$$Uhttps://www.osti.gov/biblio/1802954$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Wilhelm, Roland C.</creatorcontrib><creatorcontrib>Cyle, K. Taylor</creatorcontrib><creatorcontrib>Martinez, Carmen Enid</creatorcontrib><creatorcontrib>Karasz, David C.</creatorcontrib><creatorcontrib>Newman, Jeffrey D.</creatorcontrib><creatorcontrib>Buckley, Daniel H.</creatorcontrib><creatorcontrib>Cornell Univ., Ithaca, NY (United States)</creatorcontrib><title>Paraburkholderia solitsugae sp. nov. and Paraburkholderia elongata sp. nov., phenolic acid-degrading bacteria isolated from forest soil and emended description of Paraburkholderia madseniana</title><title>International journal of systematic and evolutionary microbiology</title><description>Two bacterial strains, 1N
T
and 5N
T
, were isolated from hemlock forest soil using a soluble organic matter enrichment. Cells of 1N
T
(0.65×1.85 µm) and 5N
T
(0.6×1.85 µm) are Gram-stain-negative, aerobic, motile, non-sporulating and exist as single rods, diplobacilli or in chains of varying length. During growth in dilute media (≤0.1× tryptic soy broth; TSB), cells are primarily motile with flagella. At higher concentrations (≥0.3× TSB), cells of both strains increasingly form non-motile chains, and cells of 5N
T
elongate (0.57×~7 µm) and form especially long filaments. Optimum growth of 1N
T
and 5N
T
occurred at 25–30 °C, pH 6.5–7.0 and <0.5% salinity. Results of comparative chemotaxonomic, genomic and phylogenetic analyses revealed that 1N
T
and 5N
T
were distinct from one another and their closest related type strains:
Paraburkholderia madseniana
RP11
T
,
Paraburkholderia aspalathi
LMG 27731
T
and
Paraburkholderia caffeinilytica
CF1
T
. The genomes of 1N
T
and 5N
T
had an average nucleotide identity (91.6 and 91.3%) and
in silico
DNA–DNA hybridization values (45.8%±2.6 and 45.5%±2.5) and differed in functional gene content from their closest related type strains. The composition of fatty acids and patterns of substrate use, including the catabolism of phenolic acids, also differentiated strains 1N
T
and 5N
T
from each other and their closest relatives. The only ubiquinone present in strains 1N
T
and 5N
T
was Q-8. The major cellular fatty acids were C
16 : 0
, 3OH-C
16 : 0
, C
17 : 0
cyclo, C
19 : 0
cyclo ω8
c
and summed features 2 (3OH-C
14 : 0
/ C
16 : 1
iso I), 3 (C
16 : 1
ω6
c
/ω7
c
) and 8 (C
18 : 1
ω7
c
/ω6
c
). A third bacterium, strain RL16-012-BIC-B, was isolated from soil associated with shallow roots and was determined to be a strain of
P. madseniana
(ANI, 98.8%; 16S rRNA gene similarity, 100%). Characterizations of strain RL16-012-BIC-B (DSM 110723=LMG 31706) led to proposed emendments to the species description of
P. madseniana
. Our polyphasic approach demonstrated that strains 1N
T
and 5N
T
represent novel species from the genus
Paraburkholderia
for which the names
Paraburkholderia solitsugae
sp. nov. (type strain 1N
T
=DSM 110721
T
=LMG 31704
T
) and
Paraburkholderia elongata
sp. nov. (type strain 5N
T
=DSM 110722
T
=LMG 31705
T
) are proposed.</description><subject>Microbiology</subject><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNplkUFP3DAQhSNUJCj02rPFqYcmHceJEx8RKhQJiR7K2ZrYk11DYgfbW6l_jt9G2K24cJrRzPfmafSK4iuHioNSP9xjormCCqARfXdUnPJGyrIF0Xx672t5UnxO6RFgHQCcFi-_MeKwi0_bMFmKDlkKk8tpt0FiaamYD38rht6yDyBNwW8w4zv2nS1b8qvcMDTOlpY2Ea3zGzagyXuNW69jJsvGGGY2hkgpr45u2lvQTN6uS0vJRLdkFzwL40fnGW0i79DjeXE84pToy_96Vjxc__xz9au8u7-5vbq8K40QKpdWSZJjLTkHq7gUqHqh-k42tTI02g464EDKQCcH3ik1YDPAYHugWtiW1-KsuDjcDSk7nYzLZLYmeE8ma95Drdpmhb4doCWG5936mZ5dMjRN6Cnskq4b0baC9_INrQ6oiSGlSKNeopsx_tMc9Fuaep-mBn1IU7wC8fuXQw</recordid><startdate>20200901</startdate><enddate>20200901</enddate><creator>Wilhelm, Roland C.</creator><creator>Cyle, K. Taylor</creator><creator>Martinez, Carmen Enid</creator><creator>Karasz, David C.</creator><creator>Newman, Jeffrey D.</creator><creator>Buckley, Daniel H.</creator><general>International Union of Microbiological Societies</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope><orcidid>https://orcid.org/0000-0003-0647-5341</orcidid><orcidid>https://orcid.org/0000-0002-1824-4017</orcidid><orcidid>https://orcid.org/0000-0003-0495-4840</orcidid><orcidid>https://orcid.org/0000-0003-1654-2907</orcidid><orcidid>https://orcid.org/0000-0001-8553-2118</orcidid><orcidid>https://orcid.org/0000000306475341</orcidid><orcidid>https://orcid.org/0000000304954840</orcidid><orcidid>https://orcid.org/0000000218244017</orcidid><orcidid>https://orcid.org/0000000185532118</orcidid><orcidid>https://orcid.org/0000000316542907</orcidid></search><sort><creationdate>20200901</creationdate><title>Paraburkholderia solitsugae sp. nov. and Paraburkholderia elongata sp. nov., phenolic acid-degrading bacteria isolated from forest soil and emended description of Paraburkholderia madseniana</title><author>Wilhelm, Roland C. ; Cyle, K. Taylor ; Martinez, Carmen Enid ; Karasz, David C. ; Newman, Jeffrey D. ; Buckley, Daniel H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c339t-d96e6f26110d9163a9839876429cefd707010e9c076b1799ba4b0bd80e23d5123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wilhelm, Roland C.</creatorcontrib><creatorcontrib>Cyle, K. Taylor</creatorcontrib><creatorcontrib>Martinez, Carmen Enid</creatorcontrib><creatorcontrib>Karasz, David C.</creatorcontrib><creatorcontrib>Newman, Jeffrey D.</creatorcontrib><creatorcontrib>Buckley, Daniel H.</creatorcontrib><creatorcontrib>Cornell Univ., Ithaca, NY (United States)</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>International journal of systematic and evolutionary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wilhelm, Roland C.</au><au>Cyle, K. Taylor</au><au>Martinez, Carmen Enid</au><au>Karasz, David C.</au><au>Newman, Jeffrey D.</au><au>Buckley, Daniel H.</au><aucorp>Cornell Univ., Ithaca, NY (United States)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Paraburkholderia solitsugae sp. nov. and Paraburkholderia elongata sp. nov., phenolic acid-degrading bacteria isolated from forest soil and emended description of Paraburkholderia madseniana</atitle><jtitle>International journal of systematic and evolutionary microbiology</jtitle><date>2020-09-01</date><risdate>2020</risdate><volume>70</volume><issue>9</issue><spage>5093</spage><epage>5105</epage><pages>5093-5105</pages><issn>1466-5026</issn><eissn>1466-5034</eissn><abstract>Two bacterial strains, 1N
T
and 5N
T
, were isolated from hemlock forest soil using a soluble organic matter enrichment. Cells of 1N
T
(0.65×1.85 µm) and 5N
T
(0.6×1.85 µm) are Gram-stain-negative, aerobic, motile, non-sporulating and exist as single rods, diplobacilli or in chains of varying length. During growth in dilute media (≤0.1× tryptic soy broth; TSB), cells are primarily motile with flagella. At higher concentrations (≥0.3× TSB), cells of both strains increasingly form non-motile chains, and cells of 5N
T
elongate (0.57×~7 µm) and form especially long filaments. Optimum growth of 1N
T
and 5N
T
occurred at 25–30 °C, pH 6.5–7.0 and <0.5% salinity. Results of comparative chemotaxonomic, genomic and phylogenetic analyses revealed that 1N
T
and 5N
T
were distinct from one another and their closest related type strains:
Paraburkholderia madseniana
RP11
T
,
Paraburkholderia aspalathi
LMG 27731
T
and
Paraburkholderia caffeinilytica
CF1
T
. The genomes of 1N
T
and 5N
T
had an average nucleotide identity (91.6 and 91.3%) and
in silico
DNA–DNA hybridization values (45.8%±2.6 and 45.5%±2.5) and differed in functional gene content from their closest related type strains. The composition of fatty acids and patterns of substrate use, including the catabolism of phenolic acids, also differentiated strains 1N
T
and 5N
T
from each other and their closest relatives. The only ubiquinone present in strains 1N
T
and 5N
T
was Q-8. The major cellular fatty acids were C
16 : 0
, 3OH-C
16 : 0
, C
17 : 0
cyclo, C
19 : 0
cyclo ω8
c
and summed features 2 (3OH-C
14 : 0
/ C
16 : 1
iso I), 3 (C
16 : 1
ω6
c
/ω7
c
) and 8 (C
18 : 1
ω7
c
/ω6
c
). A third bacterium, strain RL16-012-BIC-B, was isolated from soil associated with shallow roots and was determined to be a strain of
P. madseniana
(ANI, 98.8%; 16S rRNA gene similarity, 100%). Characterizations of strain RL16-012-BIC-B (DSM 110723=LMG 31706) led to proposed emendments to the species description of
P. madseniana
. Our polyphasic approach demonstrated that strains 1N
T
and 5N
T
represent novel species from the genus
Paraburkholderia
for which the names
Paraburkholderia solitsugae
sp. nov. (type strain 1N
T
=DSM 110721
T
=LMG 31704
T
) and
Paraburkholderia elongata
sp. nov. (type strain 5N
T
=DSM 110722
T
=LMG 31705
T
) are proposed.</abstract><cop>United States</cop><pub>International Union of Microbiological Societies</pub><doi>10.1099/ijsem.0.004387</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0003-0647-5341</orcidid><orcidid>https://orcid.org/0000-0002-1824-4017</orcidid><orcidid>https://orcid.org/0000-0003-0495-4840</orcidid><orcidid>https://orcid.org/0000-0003-1654-2907</orcidid><orcidid>https://orcid.org/0000-0001-8553-2118</orcidid><orcidid>https://orcid.org/0000000306475341</orcidid><orcidid>https://orcid.org/0000000304954840</orcidid><orcidid>https://orcid.org/0000000218244017</orcidid><orcidid>https://orcid.org/0000000185532118</orcidid><orcidid>https://orcid.org/0000000316542907</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1466-5026 |
| ispartof | International journal of systematic and evolutionary microbiology, 2020-09, Vol.70 (9), p.5093-5105 |
| issn | 1466-5026 1466-5034 |
| language | eng |
| recordid | cdi_osti_scitechconnect_1802954 |
| source | Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Microbiology |
| title | Paraburkholderia solitsugae sp. nov. and Paraburkholderia elongata sp. nov., phenolic acid-degrading bacteria isolated from forest soil and emended description of Paraburkholderia madseniana |
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