The application of flow cytometry for kelp meiospore isolation
The goal of this study was to evaluate flow-cytometric techniques for isolating individual kelp meiospores into 96-welled plates. Previously reported low success rates for sorting tens of thousands of meiospores at a time have been improved by technological developments, specifically, the low nozzle...
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| Veröffentlicht in: | Algal research (Amsterdam) 2020-01, Vol.46 |
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| container_title | Algal research (Amsterdam) |
| container_volume | 46 |
| creator | Augyte, Simona Wikfors, Gary H. Pitchford, Steve Marty-Rivera, Michael Umanzor, Schery Lindell, Scott Bailey, David Yarish, Charles |
| description | The goal of this study was to evaluate flow-cytometric techniques for isolating individual kelp meiospores into 96-welled plates. Previously reported low success rates for sorting tens of thousands of meiospores at a time have been improved by technological developments, specifically, the low nozzle pressure of the commercially available, JSAN (Bay Bioscience Co. Ltd) instrument. We monitored growth and gametophyte development post-sorting for 10 months. Our data demonstrate that successful kelp meiospore isolations of up to 76% viability can be achieved with flow-cytometry. This method can save time as compared to traditional, manual isolations using pipettes and improves confidence that self-fertilized individuals will not contaminate specific crosses of resulting gametophytes. Our results highlight a new application for the flow cytometer to produce clonal kelp gametophytes with direct applications for germplasm and culture collection development. |
| format | Article |
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Previously reported low success rates for sorting tens of thousands of meiospores at a time have been improved by technological developments, specifically, the low nozzle pressure of the commercially available, JSAN (Bay Bioscience Co. Ltd) instrument. We monitored growth and gametophyte development post-sorting for 10 months. Our data demonstrate that successful kelp meiospore isolations of up to 76% viability can be achieved with flow-cytometry. This method can save time as compared to traditional, manual isolations using pipettes and improves confidence that self-fertilized individuals will not contaminate specific crosses of resulting gametophytes. 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Previously reported low success rates for sorting tens of thousands of meiospores at a time have been improved by technological developments, specifically, the low nozzle pressure of the commercially available, JSAN (Bay Bioscience Co. Ltd) instrument. We monitored growth and gametophyte development post-sorting for 10 months. Our data demonstrate that successful kelp meiospore isolations of up to 76% viability can be achieved with flow-cytometry. This method can save time as compared to traditional, manual isolations using pipettes and improves confidence that self-fertilized individuals will not contaminate specific crosses of resulting gametophytes. Our results highlight a new application for the flow cytometer to produce clonal kelp gametophytes with direct applications for germplasm and culture collection development.</description><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>chlorophyll a</subject><subject>cytometry</subject><subject>meiospores</subject><subject>Saccharina</subject><subject>single cell sorting</subject><issn>2211-9264</issn><issn>2211-9264</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNqNirEKwjAQQIMoWLT_cLgXem1VsriI4gd0LyVcaDTNhV5A-veKODj6lveGt1BZVSEWujo0y59eq1zkXr7RDZb7MlOndiDoY_TO9MlxALZgPT_BzIlHStMMlid4kI8wkmOJPBE4Yf_Zt2pley-Uf71Ru-ulPd8KluQ6MS6RGQyHQCZ1eNQaEeu_phfgrDqn</recordid><startdate>20200129</startdate><enddate>20200129</enddate><creator>Augyte, Simona</creator><creator>Wikfors, Gary H.</creator><creator>Pitchford, Steve</creator><creator>Marty-Rivera, Michael</creator><creator>Umanzor, Schery</creator><creator>Lindell, Scott</creator><creator>Bailey, David</creator><creator>Yarish, Charles</creator><general>Elsevier</general><scope>OIOZB</scope><scope>OTOTI</scope></search><sort><creationdate>20200129</creationdate><title>The application of flow cytometry for kelp meiospore isolation</title><author>Augyte, Simona ; Wikfors, Gary H. ; Pitchford, Steve ; Marty-Rivera, Michael ; Umanzor, Schery ; Lindell, Scott ; Bailey, David ; Yarish, Charles</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-osti_scitechconnect_17991113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>chlorophyll a</topic><topic>cytometry</topic><topic>meiospores</topic><topic>Saccharina</topic><topic>single cell sorting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Augyte, Simona</creatorcontrib><creatorcontrib>Wikfors, Gary H.</creatorcontrib><creatorcontrib>Pitchford, Steve</creatorcontrib><creatorcontrib>Marty-Rivera, Michael</creatorcontrib><creatorcontrib>Umanzor, Schery</creatorcontrib><creatorcontrib>Lindell, Scott</creatorcontrib><creatorcontrib>Bailey, David</creatorcontrib><creatorcontrib>Yarish, Charles</creatorcontrib><creatorcontrib>Woods Hole Oceanographic Institution, Woods Hole, MA (United States)</creatorcontrib><collection>OSTI.GOV - Hybrid</collection><collection>OSTI.GOV</collection><jtitle>Algal research (Amsterdam)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Augyte, Simona</au><au>Wikfors, Gary H.</au><au>Pitchford, Steve</au><au>Marty-Rivera, Michael</au><au>Umanzor, Schery</au><au>Lindell, Scott</au><au>Bailey, David</au><au>Yarish, Charles</au><aucorp>Woods Hole Oceanographic Institution, Woods Hole, MA (United States)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The application of flow cytometry for kelp meiospore isolation</atitle><jtitle>Algal research (Amsterdam)</jtitle><date>2020-01-29</date><risdate>2020</risdate><volume>46</volume><issn>2211-9264</issn><eissn>2211-9264</eissn><abstract>The goal of this study was to evaluate flow-cytometric techniques for isolating individual kelp meiospores into 96-welled plates. 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| fulltext | fulltext |
| identifier | ISSN: 2211-9264 |
| ispartof | Algal research (Amsterdam), 2020-01, Vol.46 |
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| language | eng |
| recordid | cdi_osti_scitechconnect_1799111 |
| source | Alma/SFX Local Collection |
| subjects | BASIC BIOLOGICAL SCIENCES chlorophyll a cytometry meiospores Saccharina single cell sorting |
| title | The application of flow cytometry for kelp meiospore isolation |
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