Programmable Aggregation of Artificial Cells with DNA Signals
Cell aggregation is a complex behavior that is closely related to the viability, differentiation, and migration of cells. An effort to create synthetic analogs could lead to considerable advances in cell physiology and biophysics. Rendering and modulating such a dynamic artificial cell system requir...
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| Veröffentlicht in: | ACS synthetic biology 2021-06, Vol.10 (6), p.1268-1276 |
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| container_title | ACS synthetic biology |
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| creator | Qiu, Hengming Li, Feiran Du, Yancheng Li, Ruixin Hyun, Ji Yeon Lee, Sei Young Choi, Jong Hyun |
| description | Cell aggregation is a complex behavior that is closely related to the viability, differentiation, and migration of cells. An effort to create synthetic analogs could lead to considerable advances in cell physiology and biophysics. Rendering and modulating such a dynamic artificial cell system require mechanisms for receiving, transducing, and transmitting intercellular signals, yet effective tools are limited at present. Here we construct synthetic cells from engineered lipids and show their programmable aggregation behaviors using DNA oligonucleotides as signaling molecules. The artificial cells have transmembrane channels made of DNA origami that are used to recognize and process intercellular signals. We demonstrate that multiple small vesicles aggregate onto a giant vesicle after a transduction of external DNA signals by an intracellular enzyme and that the small vesicles dissociate when receiving “release” signals. This work provides new possibilities for building synthetic protocells capable of chemical communication and coordination. |
| doi_str_mv | 10.1021/acssynbio.0c00550 |
| format | Article |
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An effort to create synthetic analogs could lead to considerable advances in cell physiology and biophysics. Rendering and modulating such a dynamic artificial cell system require mechanisms for receiving, transducing, and transmitting intercellular signals, yet effective tools are limited at present. Here we construct synthetic cells from engineered lipids and show their programmable aggregation behaviors using DNA oligonucleotides as signaling molecules. The artificial cells have transmembrane channels made of DNA origami that are used to recognize and process intercellular signals. We demonstrate that multiple small vesicles aggregate onto a giant vesicle after a transduction of external DNA signals by an intracellular enzyme and that the small vesicles dissociate when receiving “release” signals. 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| source | ACS Publications; MEDLINE |
| subjects | aggregation Artificial Cells - metabolism Base Sequence BASIC BIOLOGICAL SCIENCES cell aggregation Cell Aggregation - genetics DNA - chemistry DNA - metabolism DNA nanotechnology DNA origami Extracellular Space - metabolism fluorescence Genetic Engineering - methods genetics Ion Channels - metabolism Lipids - genetics membrane pore molecules Nanostructures - chemistry Nanotechnology - methods Oligonucleotides - metabolism Signal Transduction - genetics synthetic cells Transport Vesicles - metabolism vesicles |
| title | Programmable Aggregation of Artificial Cells with DNA Signals |
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