Molecular characterization of an NADPH cytochrome P450 reductase from Bemisia tabaci Q: Potential involvement in susceptibility to imidacloprid
NADPH cytochrome P450 reductase (CPR) is an integral component of cytochrome P450-mediated biological reactions, such as the metabolism of xenobiotics, including insecticides. CPR has been reported to be associated with insecticide tolerance in several insects. However, the biochemical characteristi...
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| Veröffentlicht in: | Pesticide biochemistry and physiology 2020-01, Vol.162 (C), p.29-35 |
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| creator | He, Chao Liang, Jinjin Liu, Shaonan Zeng, Yang Wang, Shaoli Wu, Qingjun Xie, Wen Zhang, Youjun |
| description | NADPH cytochrome P450 reductase (CPR) is an integral component of cytochrome P450-mediated biological reactions, such as the metabolism of xenobiotics, including insecticides. CPR has been reported to be associated with insecticide tolerance in several insects. However, the biochemical characteristics and biological function of CPR in Bemisia tabaci Q (BtCPR) remain undefined. In this study, BtCPR was cloned, and bioinformatic analysis showed that BtCPR is a transmembrane protein with a molecular weight (MW) of 76.73 kDa and contains conserved binding domains (FMN, FAD, and NADPH). Tissue- and developmental stage-dependent expression indicated that the highest expression levels of BtCPR occurred in head tissue and in male adults. Transcripts of BtCPR in the field B. tabaci Q strain were 1.62-fold higher than those of the laboratory B. tabaci Q strain. In both field and laboratory adults, the susceptibility of BtCPR-knockdown B. tabaci Q to imidacloprid substantially increased compared to that of the B. tabaci Q control group. Furthermore, the heterologous expression of BtCPR in Sf9 cells exhibited catalytic activity for cytochrome c reduction, following Michaelis-Menten kinetics. Sf9 cells overexpressing BtCPR had greater viability than the control cells when treated with imidacloprid. The results suggest that BtCPR could affect the susceptibility of B. tabaci Q to imidacloprid and could also be considered a novel target for pest control.
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•Full-length cDNA of BtCPR gene were cloned and characterized in Bemisia tabaci Q•In both field and laboratory adults, the susceptibility of BtCPR-knockdown B. tabaci Q to imidacloprid substantially increased compared to that of the B. tabaci Q control group.•Heterologous expression of BtCPR in Sf9 cells exhibited catalytic activity for cytochrome c reduction and had greater viability than the control cells when treated with imidacloprid |
| doi_str_mv | 10.1016/j.pestbp.2019.07.018 |
| format | Article |
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[Display omitted]
•Full-length cDNA of BtCPR gene were cloned and characterized in Bemisia tabaci Q•In both field and laboratory adults, the susceptibility of BtCPR-knockdown B. tabaci Q to imidacloprid substantially increased compared to that of the B. tabaci Q control group.•Heterologous expression of BtCPR in Sf9 cells exhibited catalytic activity for cytochrome c reduction and had greater viability than the control cells when treated with imidacloprid</description><identifier>ISSN: 0048-3575</identifier><identifier>EISSN: 1095-9939</identifier><identifier>DOI: 10.1016/j.pestbp.2019.07.018</identifier><identifier>PMID: 31836051</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Bemisia tabaci ; CPR ; Cytochrome P-450 Enzyme System ; Hemiptera ; Imidacloprid ; Imidazoles ; Insecticide Resistance ; Insecticides ; Male ; NADPH-Ferrihemoprotein Reductase ; Neonicotinoids ; Nitro Compounds ; RNAi</subject><ispartof>Pesticide biochemistry and physiology, 2020-01, Vol.162 (C), p.29-35</ispartof><rights>2019 Elsevier Inc.</rights><rights>Copyright © 2019 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c435t-95d553ee2f5a7f6f5222cb9a5ef2177d3087abd777ac76ff476b5187e3a655233</citedby><cites>FETCH-LOGICAL-c435t-95d553ee2f5a7f6f5222cb9a5ef2177d3087abd777ac76ff476b5187e3a655233</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.pestbp.2019.07.018$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31836051$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/1682453$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>He, Chao</creatorcontrib><creatorcontrib>Liang, Jinjin</creatorcontrib><creatorcontrib>Liu, Shaonan</creatorcontrib><creatorcontrib>Zeng, Yang</creatorcontrib><creatorcontrib>Wang, Shaoli</creatorcontrib><creatorcontrib>Wu, Qingjun</creatorcontrib><creatorcontrib>Xie, Wen</creatorcontrib><creatorcontrib>Zhang, Youjun</creatorcontrib><title>Molecular characterization of an NADPH cytochrome P450 reductase from Bemisia tabaci Q: Potential involvement in susceptibility to imidacloprid</title><title>Pesticide biochemistry and physiology</title><addtitle>Pestic Biochem Physiol</addtitle><description>NADPH cytochrome P450 reductase (CPR) is an integral component of cytochrome P450-mediated biological reactions, such as the metabolism of xenobiotics, including insecticides. CPR has been reported to be associated with insecticide tolerance in several insects. However, the biochemical characteristics and biological function of CPR in Bemisia tabaci Q (BtCPR) remain undefined. In this study, BtCPR was cloned, and bioinformatic analysis showed that BtCPR is a transmembrane protein with a molecular weight (MW) of 76.73 kDa and contains conserved binding domains (FMN, FAD, and NADPH). Tissue- and developmental stage-dependent expression indicated that the highest expression levels of BtCPR occurred in head tissue and in male adults. Transcripts of BtCPR in the field B. tabaci Q strain were 1.62-fold higher than those of the laboratory B. tabaci Q strain. In both field and laboratory adults, the susceptibility of BtCPR-knockdown B. tabaci Q to imidacloprid substantially increased compared to that of the B. tabaci Q control group. Furthermore, the heterologous expression of BtCPR in Sf9 cells exhibited catalytic activity for cytochrome c reduction, following Michaelis-Menten kinetics. Sf9 cells overexpressing BtCPR had greater viability than the control cells when treated with imidacloprid. The results suggest that BtCPR could affect the susceptibility of B. tabaci Q to imidacloprid and could also be considered a novel target for pest control.
[Display omitted]
•Full-length cDNA of BtCPR gene were cloned and characterized in Bemisia tabaci Q•In both field and laboratory adults, the susceptibility of BtCPR-knockdown B. tabaci Q to imidacloprid substantially increased compared to that of the B. tabaci Q control group.•Heterologous expression of BtCPR in Sf9 cells exhibited catalytic activity for cytochrome c reduction and had greater viability than the control cells when treated with imidacloprid</description><subject>Animals</subject><subject>Bemisia tabaci</subject><subject>CPR</subject><subject>Cytochrome P-450 Enzyme System</subject><subject>Hemiptera</subject><subject>Imidacloprid</subject><subject>Imidazoles</subject><subject>Insecticide Resistance</subject><subject>Insecticides</subject><subject>Male</subject><subject>NADPH-Ferrihemoprotein Reductase</subject><subject>Neonicotinoids</subject><subject>Nitro Compounds</subject><subject>RNAi</subject><issn>0048-3575</issn><issn>1095-9939</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcuO1SAYgInROMfRNzCGuHLTCqWU4sJkHC9jMuox0TWh9CeHk7Z0gJ7k-BK-sjQdXbriku-_fgg9p6SkhDavj-UMMXVzWREqSyJKQtsHaEeJ5IWUTD5EO0LqtmBc8Av0JMYjIUTWRD5GF4y2rCGc7tDvL34Asww6YHPQQZsEwf3SyfkJe4v1hL9evd_fYHNO3hyCHwHva05wgH4xSUfANn_idzC66DROutPG4e9v8N4nmJLTA3bTyQ8nGPMz33FcooE5uc4NLp1x8tiNrtdm8HNw_VP0yOohwrP78xL9_Pjhx_VNcfvt0-frq9vC1IynQvKecwZQWa6FbSyvqsp0UnOwFRWiZ6QVuuuFENqIxtpaNB2nrQCmG84rxi7Ryy2vj8mpaFwCczB-msAkRZu2qvkKvdqgOfi7JW9b5SkNDIOewC9R5TyNlC1jJKP1hprgYwxgVZ5m1OGsKFGrL3VUmy-1-lJEqOwrh724r7B0I_T_gv4KysDbDYC8jJODsPYKk4HehbXV3rv_V_gDJTOqLg</recordid><startdate>202001</startdate><enddate>202001</enddate><creator>He, Chao</creator><creator>Liang, Jinjin</creator><creator>Liu, Shaonan</creator><creator>Zeng, Yang</creator><creator>Wang, Shaoli</creator><creator>Wu, Qingjun</creator><creator>Xie, Wen</creator><creator>Zhang, Youjun</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>202001</creationdate><title>Molecular characterization of an NADPH cytochrome P450 reductase from Bemisia tabaci Q: Potential involvement in susceptibility to imidacloprid</title><author>He, Chao ; Liang, Jinjin ; Liu, Shaonan ; Zeng, Yang ; Wang, Shaoli ; Wu, Qingjun ; Xie, Wen ; Zhang, Youjun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c435t-95d553ee2f5a7f6f5222cb9a5ef2177d3087abd777ac76ff476b5187e3a655233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Bemisia tabaci</topic><topic>CPR</topic><topic>Cytochrome P-450 Enzyme System</topic><topic>Hemiptera</topic><topic>Imidacloprid</topic><topic>Imidazoles</topic><topic>Insecticide Resistance</topic><topic>Insecticides</topic><topic>Male</topic><topic>NADPH-Ferrihemoprotein Reductase</topic><topic>Neonicotinoids</topic><topic>Nitro Compounds</topic><topic>RNAi</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>He, Chao</creatorcontrib><creatorcontrib>Liang, Jinjin</creatorcontrib><creatorcontrib>Liu, Shaonan</creatorcontrib><creatorcontrib>Zeng, Yang</creatorcontrib><creatorcontrib>Wang, Shaoli</creatorcontrib><creatorcontrib>Wu, Qingjun</creatorcontrib><creatorcontrib>Xie, Wen</creatorcontrib><creatorcontrib>Zhang, Youjun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Pesticide biochemistry and physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>He, Chao</au><au>Liang, Jinjin</au><au>Liu, Shaonan</au><au>Zeng, Yang</au><au>Wang, Shaoli</au><au>Wu, Qingjun</au><au>Xie, Wen</au><au>Zhang, Youjun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization of an NADPH cytochrome P450 reductase from Bemisia tabaci Q: Potential involvement in susceptibility to imidacloprid</atitle><jtitle>Pesticide biochemistry and physiology</jtitle><addtitle>Pestic Biochem Physiol</addtitle><date>2020-01</date><risdate>2020</risdate><volume>162</volume><issue>C</issue><spage>29</spage><epage>35</epage><pages>29-35</pages><issn>0048-3575</issn><eissn>1095-9939</eissn><abstract>NADPH cytochrome P450 reductase (CPR) is an integral component of cytochrome P450-mediated biological reactions, such as the metabolism of xenobiotics, including insecticides. CPR has been reported to be associated with insecticide tolerance in several insects. However, the biochemical characteristics and biological function of CPR in Bemisia tabaci Q (BtCPR) remain undefined. In this study, BtCPR was cloned, and bioinformatic analysis showed that BtCPR is a transmembrane protein with a molecular weight (MW) of 76.73 kDa and contains conserved binding domains (FMN, FAD, and NADPH). Tissue- and developmental stage-dependent expression indicated that the highest expression levels of BtCPR occurred in head tissue and in male adults. Transcripts of BtCPR in the field B. tabaci Q strain were 1.62-fold higher than those of the laboratory B. tabaci Q strain. In both field and laboratory adults, the susceptibility of BtCPR-knockdown B. tabaci Q to imidacloprid substantially increased compared to that of the B. tabaci Q control group. Furthermore, the heterologous expression of BtCPR in Sf9 cells exhibited catalytic activity for cytochrome c reduction, following Michaelis-Menten kinetics. Sf9 cells overexpressing BtCPR had greater viability than the control cells when treated with imidacloprid. The results suggest that BtCPR could affect the susceptibility of B. tabaci Q to imidacloprid and could also be considered a novel target for pest control.
[Display omitted]
•Full-length cDNA of BtCPR gene were cloned and characterized in Bemisia tabaci Q•In both field and laboratory adults, the susceptibility of BtCPR-knockdown B. tabaci Q to imidacloprid substantially increased compared to that of the B. tabaci Q control group.•Heterologous expression of BtCPR in Sf9 cells exhibited catalytic activity for cytochrome c reduction and had greater viability than the control cells when treated with imidacloprid</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>31836051</pmid><doi>10.1016/j.pestbp.2019.07.018</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Bemisia tabaci CPR Cytochrome P-450 Enzyme System Hemiptera Imidacloprid Imidazoles Insecticide Resistance Insecticides Male NADPH-Ferrihemoprotein Reductase Neonicotinoids Nitro Compounds RNAi |
| title | Molecular characterization of an NADPH cytochrome P450 reductase from Bemisia tabaci Q: Potential involvement in susceptibility to imidacloprid |
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