Mix-and-inject XFEL crystallography reveals gated conformational dynamics during enzyme catalysis

How changes in enzyme structure and dynamics facilitate passage along the reaction coordinate is a fundamental unanswered question. Here, we use time-resolved mix-and-inject serial crystallography (MISC) at an X-ray free electron laser (XFEL), ambient-temperature X-ray crystallography, computer simu...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2019-12, Vol.116 (51)
Hauptverfasser:	Dasgupta, Medhanjali, Budday, Dominik, de Oliveira, Saulo P., Madzelan, Peter, Marchany-Rivera, Darya, Seravalli, Javier, Hayes, Brandon, Sierra, Raymond G., Boutet, Sébastien, Hunter, Mark S., Alonso-Mori, Roberto, Batyuk, Alexander, Wierman, Jennifer, Lyubimov, Artem, Brewster, Aaron S., Sauter, Nicholas K., Applegate, Gregory A., Tiwari, Virendra K., Berkowitz, David B., Thompson, Michael C., Cohen, Aina E., Fraser, James S., Wall, Michael E., van den Bedem, Henry, Wilson, Mark A.
Format:	Artikel
Sprache:	eng
Schlagworte:	cysteine modification dynamics enzyme conformational enzyme conformational dynamics MATERIALS SCIENCE radiation damage X-ray crystallography XFEL
Online-Zugang:	Volltext
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