Structure-based Engineering of a Plant-Fungal Hybrid Peroxidase for Enhanced Temperature and pH Tolerance

In an age of ever-increasing biotechnological and industrial demand for new and specialized biocatalysts, rational protein engineering offers a direct approach to enzyme design and innovation. Heme peroxidases, as indispensable oxidative biocatalysts, provide a relatively mild alternative to the tra...

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Veröffentlicht in:	Cell chemical biology 2018-08, Vol.25 (8), p.974-983.e3
Hauptverfasser:	Kohler, Amanda C., Simmons, Blake A., Sale, Kenneth L.
Format:	Artikel
Sprache:	eng
Schlagworte:	biocatalysis biofuels Biotechnology enzyme engineering Enzyme Stability enzymes Escherichia coli - genetics Hydrogen-Ion Concentration lignin Models, Molecular oxidation Oxidation-Reduction Peroxidase - chemistry Peroxidase - genetics Peroxidase - metabolism peroxidases Plants - chemistry Plants - enzymology Plants - genetics Plants - metabolism Pleurotus - chemistry Pleurotus - enzymology Pleurotus - genetics Pleurotus - metabolism Protein Conformation Protein Engineering - methods Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Substrate Specificity Temperature
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