The homothallic mating-type locus of the conifer needle endophyte Phialocephala scopiformis DAOMC 229536 (order Helotiales)
We describe the complete mating-type (MAT) locus for Phialocephala scopiformis Canadian Collection of Fungal Cultures (DAOMC) 229536 – a basal lineage within Vibrisseaceae. This strain is of interest due to its ability to produce the important antiinsectan rugulosin. We also provide some of the firs...
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description | We describe the complete mating-type (MAT) locus for Phialocephala scopiformis Canadian Collection of Fungal Cultures (DAOMC) 229536 – a basal lineage within Vibrisseaceae. This strain is of interest due to its ability to produce the important antiinsectan rugulosin. We also provide some of the first insights into the genome structure and gene inventory of nonclavicipitalean endophytes. Sequence was obtained through shotgun sequencing of the entire P. scopiformis genome, and the MAT locus was then determined by comparing this genomic sequence to known MAT loci within the Phialocephala fortinii s.l.–Acephala applanata species complex. We also tested the relative levels of sequence conservation for MAT genes within Vibrisseaceae (n = 10), as well as within the Helotiales (n = 27). Our results: (1) show a homothallic gene arrangement for P. scopiformis [MAT1-1-1, MAT1-2-1, and MAT1-1-3 genes are present], (2) increase the genomic survey of homothallism within Vibrisseaceae, (3) confirm that P. scopiformis contains a unique S-adenosyl-l-methionine-dependent methyltransferase (SAM-Mtase) gene proximal to its MAT locus, while also lacking a cytoskeleton assembly control protein (sla2) gene, and (4) indicate that MAT1-1-1 is the more useful molecular marker amongst the MAT genes for phylogenetic reconstructions aimed at tracking evolutionary shifts in reproductive strategy and/or MAT loci gene composition within the Helotiales.
•Phialocephala scopiformis is homothallic.•Homothallism is confirmed in more than one member of the Vibrisseaceae.•The MAT locus of P. scopiformis is unusual, containing SAM-Mtase gene but not sla2.•MAT1-1-1 gene is the most useful MAT gene for helotialean phylogenetic analysis. |
doi_str_mv | 10.1016/j.funbio.2017.08.007 |
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•Phialocephala scopiformis is homothallic.•Homothallism is confirmed in more than one member of the Vibrisseaceae.•The MAT locus of P. scopiformis is unusual, containing SAM-Mtase gene but not sla2.•MAT1-1-1 gene is the most useful MAT gene for helotialean phylogenetic analysis.</description><identifier>ISSN: 1878-6146</identifier><identifier>EISSN: 1878-6162</identifier><identifier>DOI: 10.1016/j.funbio.2017.08.007</identifier><identifier>PMID: 29122173</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Ascomycete ; Ascomycota - genetics ; Coniferophyta - microbiology ; Conserved Sequence ; DNA, Fungal - chemistry ; DNA, Fungal - genetics ; Endophytes - genetics ; Gene Order ; Genes, Mating Type, Fungal ; Genetic Loci ; Genome, Fungal ; Idiomorph ; MAT gene ; Non-clavicipitalean ; Plant Leaves - microbiology ; Rugulosin ; Sequence Analysis, DNA ; Sequence Homology</subject><ispartof>Fungal biology, 2017-12, Vol.121 (12), p.1011-1024</ispartof><rights>2017 British Mycological Society</rights><rights>Copyright © 2017 British Mycological Society. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c435t-e1d96a6d159f3d15067735d18c4464dad2cd7204a0cc9276f27db89cf07c5dc33</citedby><cites>FETCH-LOGICAL-c435t-e1d96a6d159f3d15067735d18c4464dad2cd7204a0cc9276f27db89cf07c5dc33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1878614617301101$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29122173$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/1549799$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Robicheau, Brent M.</creatorcontrib><creatorcontrib>Bunbury-Blanchette, Adèle L.</creatorcontrib><creatorcontrib>LaButti, Kurt</creatorcontrib><creatorcontrib>Grigoriev, Igor V.</creatorcontrib><creatorcontrib>Walker, Allison K.</creatorcontrib><title>The homothallic mating-type locus of the conifer needle endophyte Phialocephala scopiformis DAOMC 229536 (order Helotiales)</title><title>Fungal biology</title><addtitle>Fungal Biol</addtitle><description>We describe the complete mating-type (MAT) locus for Phialocephala scopiformis Canadian Collection of Fungal Cultures (DAOMC) 229536 – a basal lineage within Vibrisseaceae. This strain is of interest due to its ability to produce the important antiinsectan rugulosin. We also provide some of the first insights into the genome structure and gene inventory of nonclavicipitalean endophytes. Sequence was obtained through shotgun sequencing of the entire P. scopiformis genome, and the MAT locus was then determined by comparing this genomic sequence to known MAT loci within the Phialocephala fortinii s.l.–Acephala applanata species complex. We also tested the relative levels of sequence conservation for MAT genes within Vibrisseaceae (n = 10), as well as within the Helotiales (n = 27). Our results: (1) show a homothallic gene arrangement for P. scopiformis [MAT1-1-1, MAT1-2-1, and MAT1-1-3 genes are present], (2) increase the genomic survey of homothallism within Vibrisseaceae, (3) confirm that P. scopiformis contains a unique S-adenosyl-l-methionine-dependent methyltransferase (SAM-Mtase) gene proximal to its MAT locus, while also lacking a cytoskeleton assembly control protein (sla2) gene, and (4) indicate that MAT1-1-1 is the more useful molecular marker amongst the MAT genes for phylogenetic reconstructions aimed at tracking evolutionary shifts in reproductive strategy and/or MAT loci gene composition within the Helotiales.
•Phialocephala scopiformis is homothallic.•Homothallism is confirmed in more than one member of the Vibrisseaceae.•The MAT locus of P. scopiformis is unusual, containing SAM-Mtase gene but not sla2.•MAT1-1-1 gene is the most useful MAT gene for helotialean phylogenetic analysis.</description><subject>Ascomycete</subject><subject>Ascomycota - genetics</subject><subject>Coniferophyta - microbiology</subject><subject>Conserved Sequence</subject><subject>DNA, Fungal - chemistry</subject><subject>DNA, Fungal - genetics</subject><subject>Endophytes - genetics</subject><subject>Gene Order</subject><subject>Genes, Mating Type, Fungal</subject><subject>Genetic Loci</subject><subject>Genome, Fungal</subject><subject>Idiomorph</subject><subject>MAT gene</subject><subject>Non-clavicipitalean</subject><subject>Plant Leaves - microbiology</subject><subject>Rugulosin</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology</subject><issn>1878-6146</issn><issn>1878-6162</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctu1DAUhiMEolXpGyBksSqLBF8SO94gVQO0SEVlUdaWxz4mHiVxsB2kES-PR2m7xIvjs_j-c_ur6i3BDcGEfzw0bp33PjQUE9HgvsFYvKjOSS_6mhNOXz7nLT-rLlM64PIYYb0Ur6szKgmlRLDz6u_DAGgIU8iDHkdv0KSzn3_V-bgAGoNZEwoO5QKZMHsHEc0AdgQEsw3LcMyAfgxeFxKWUkGjZMLiXYiTT-jz9f33HaJUdoyjqxBtkd_CGHIRQPrwpnrl9Jjg8vG_qH5-_fKwu63v7m--7a7vatOyLtdArOSaW9JJx0rEXAjWWdKbtuWt1ZYaKyhuNTZGUsEdFXbfS-OwMJ01jF1U77e6IWWvkvEZzFDWmcFkRbpWCikLdLVBSwy_V0hZlQ0MjKOeIaxJEckZFZj3XUHbDTUxpBTBqSX6ScejIlid7FEHtdmjTvYo3KtiT5G9e-yw7iewz6InMwrwaQOgHOOPh3iaFWYD1sfTqDb4_3f4BwZdohs</recordid><startdate>201712</startdate><enddate>201712</enddate><creator>Robicheau, Brent M.</creator><creator>Bunbury-Blanchette, Adèle L.</creator><creator>LaButti, Kurt</creator><creator>Grigoriev, Igor V.</creator><creator>Walker, Allison K.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>201712</creationdate><title>The homothallic mating-type locus of the conifer needle endophyte Phialocephala scopiformis DAOMC 229536 (order Helotiales)</title><author>Robicheau, Brent M. ; Bunbury-Blanchette, Adèle L. ; LaButti, Kurt ; Grigoriev, Igor V. ; Walker, Allison K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c435t-e1d96a6d159f3d15067735d18c4464dad2cd7204a0cc9276f27db89cf07c5dc33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Ascomycete</topic><topic>Ascomycota - genetics</topic><topic>Coniferophyta - microbiology</topic><topic>Conserved Sequence</topic><topic>DNA, Fungal - chemistry</topic><topic>DNA, Fungal - genetics</topic><topic>Endophytes - genetics</topic><topic>Gene Order</topic><topic>Genes, Mating Type, Fungal</topic><topic>Genetic Loci</topic><topic>Genome, Fungal</topic><topic>Idiomorph</topic><topic>MAT gene</topic><topic>Non-clavicipitalean</topic><topic>Plant Leaves - microbiology</topic><topic>Rugulosin</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Robicheau, Brent M.</creatorcontrib><creatorcontrib>Bunbury-Blanchette, Adèle L.</creatorcontrib><creatorcontrib>LaButti, Kurt</creatorcontrib><creatorcontrib>Grigoriev, Igor V.</creatorcontrib><creatorcontrib>Walker, Allison K.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Fungal biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Robicheau, Brent M.</au><au>Bunbury-Blanchette, Adèle L.</au><au>LaButti, Kurt</au><au>Grigoriev, Igor V.</au><au>Walker, Allison K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The homothallic mating-type locus of the conifer needle endophyte Phialocephala scopiformis DAOMC 229536 (order Helotiales)</atitle><jtitle>Fungal biology</jtitle><addtitle>Fungal Biol</addtitle><date>2017-12</date><risdate>2017</risdate><volume>121</volume><issue>12</issue><spage>1011</spage><epage>1024</epage><pages>1011-1024</pages><issn>1878-6146</issn><eissn>1878-6162</eissn><abstract>We describe the complete mating-type (MAT) locus for Phialocephala scopiformis Canadian Collection of Fungal Cultures (DAOMC) 229536 – a basal lineage within Vibrisseaceae. This strain is of interest due to its ability to produce the important antiinsectan rugulosin. We also provide some of the first insights into the genome structure and gene inventory of nonclavicipitalean endophytes. Sequence was obtained through shotgun sequencing of the entire P. scopiformis genome, and the MAT locus was then determined by comparing this genomic sequence to known MAT loci within the Phialocephala fortinii s.l.–Acephala applanata species complex. We also tested the relative levels of sequence conservation for MAT genes within Vibrisseaceae (n = 10), as well as within the Helotiales (n = 27). Our results: (1) show a homothallic gene arrangement for P. scopiformis [MAT1-1-1, MAT1-2-1, and MAT1-1-3 genes are present], (2) increase the genomic survey of homothallism within Vibrisseaceae, (3) confirm that P. scopiformis contains a unique S-adenosyl-l-methionine-dependent methyltransferase (SAM-Mtase) gene proximal to its MAT locus, while also lacking a cytoskeleton assembly control protein (sla2) gene, and (4) indicate that MAT1-1-1 is the more useful molecular marker amongst the MAT genes for phylogenetic reconstructions aimed at tracking evolutionary shifts in reproductive strategy and/or MAT loci gene composition within the Helotiales.
•Phialocephala scopiformis is homothallic.•Homothallism is confirmed in more than one member of the Vibrisseaceae.•The MAT locus of P. scopiformis is unusual, containing SAM-Mtase gene but not sla2.•MAT1-1-1 gene is the most useful MAT gene for helotialean phylogenetic analysis.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>29122173</pmid><doi>10.1016/j.funbio.2017.08.007</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Ascomycete Ascomycota - genetics Coniferophyta - microbiology Conserved Sequence DNA, Fungal - chemistry DNA, Fungal - genetics Endophytes - genetics Gene Order Genes, Mating Type, Fungal Genetic Loci Genome, Fungal Idiomorph MAT gene Non-clavicipitalean Plant Leaves - microbiology Rugulosin Sequence Analysis, DNA Sequence Homology |
title | The homothallic mating-type locus of the conifer needle endophyte Phialocephala scopiformis DAOMC 229536 (order Helotiales) |
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