The homothallic mating-type locus of the conifer needle endophyte Phialocephala scopiformis DAOMC 229536 (order Helotiales)

We describe the complete mating-type (MAT) locus for Phialocephala scopiformis Canadian Collection of Fungal Cultures (DAOMC) 229536 – a basal lineage within Vibrisseaceae. This strain is of interest due to its ability to produce the important antiinsectan rugulosin. We also provide some of the firs...

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Veröffentlicht in:Fungal biology 2017-12, Vol.121 (12), p.1011-1024
Hauptverfasser: Robicheau, Brent M., Bunbury-Blanchette, Adèle L., LaButti, Kurt, Grigoriev, Igor V., Walker, Allison K.
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container_end_page 1024
container_issue 12
container_start_page 1011
container_title Fungal biology
container_volume 121
creator Robicheau, Brent M.
Bunbury-Blanchette, Adèle L.
LaButti, Kurt
Grigoriev, Igor V.
Walker, Allison K.
description We describe the complete mating-type (MAT) locus for Phialocephala scopiformis Canadian Collection of Fungal Cultures (DAOMC) 229536 – a basal lineage within Vibrisseaceae. This strain is of interest due to its ability to produce the important antiinsectan rugulosin. We also provide some of the first insights into the genome structure and gene inventory of nonclavicipitalean endophytes. Sequence was obtained through shotgun sequencing of the entire P. scopiformis genome, and the MAT locus was then determined by comparing this genomic sequence to known MAT loci within the Phialocephala fortinii s.l.–Acephala applanata species complex. We also tested the relative levels of sequence conservation for MAT genes within Vibrisseaceae (n = 10), as well as within the Helotiales (n = 27). Our results: (1) show a homothallic gene arrangement for P. scopiformis [MAT1-1-1, MAT1-2-1, and MAT1-1-3 genes are present], (2) increase the genomic survey of homothallism within Vibrisseaceae, (3) confirm that P. scopiformis contains a unique S-adenosyl-l-methionine-dependent methyltransferase (SAM-Mtase) gene proximal to its MAT locus, while also lacking a cytoskeleton assembly control protein (sla2) gene, and (4) indicate that MAT1-1-1 is the more useful molecular marker amongst the MAT genes for phylogenetic reconstructions aimed at tracking evolutionary shifts in reproductive strategy and/or MAT loci gene composition within the Helotiales. •Phialocephala scopiformis is homothallic.•Homothallism is confirmed in more than one member of the Vibrisseaceae.•The MAT locus of P. scopiformis is unusual, containing SAM-Mtase gene but not sla2.•MAT1-1-1 gene is the most useful MAT gene for helotialean phylogenetic analysis.
doi_str_mv 10.1016/j.funbio.2017.08.007
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ispartof Fungal biology, 2017-12, Vol.121 (12), p.1011-1024
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language eng
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source MEDLINE; Elsevier ScienceDirect Journals Complete
subjects Ascomycete
Ascomycota - genetics
Coniferophyta - microbiology
Conserved Sequence
DNA, Fungal - chemistry
DNA, Fungal - genetics
Endophytes - genetics
Gene Order
Genes, Mating Type, Fungal
Genetic Loci
Genome, Fungal
Idiomorph
MAT gene
Non-clavicipitalean
Plant Leaves - microbiology
Rugulosin
Sequence Analysis, DNA
Sequence Homology
title The homothallic mating-type locus of the conifer needle endophyte Phialocephala scopiformis DAOMC 229536 (order Helotiales)
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