Engineering a thermostable highly active glucose 6-phosphate dehydrogenase and its application to hydrogen production in vitro

Glucose 6-phosphate dehydrogenase (G6PDH) is one of the most important dehydrogenases responsible for generating reduced NADPH for anabolism and is also the rate-limiting enzyme in the Entner-Doudoroff pathway. For in vitro biocatalysis, G6PDH must possess both high activity and good thermostability...

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Veröffentlicht in:	Applied microbiology and biotechnology 2018-04, Vol.102 (7), p.3203-3215
Hauptverfasser:	Huang, Rui, Chen, Hui, Zhou, Wei, Ma, Chunling, Zhang, Y.-H. Percival
Format:	Artikel
Sprache:	eng
Schlagworte:	Bioengineering Biomedical and Life Sciences Biotechnologically Relevant Enzymes and Proteins Biotechnology Biotechnology & Applied Microbiology Catalysis Dehydrogenase Dehydrogenases Directed evolution Entner-Doudoroff pathway Enzyme Stability Enzymes Evolution Glucose 6 phosphate dehydrogenase Glucosephosphate dehydrogenase Glucosephosphate Dehydrogenase - genetics Glucosephosphate Dehydrogenase - metabolism High temperature Hot Temperature Hydrogen Hydrogen - metabolism Hydrogen production Industrial Microbiology Life Sciences Maltodextrin Melt temperature Methods Microbial Genetics and Genomics Microbiology NAD NADP Oxidoreductases Physiological aspects Polysaccharides - metabolism Production processes Protein Engineering Random mutagenesis Regeneration Thermal properties Thermal stability Zymomonas Zymomonas mobilis
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