A molecular rheostat maintains ATP levels to drive a synthetic biochemistry system

An enzymatic rheostat controls ATP levels by regulating flux through two pathways depending on free phosphate levels. Incorporation of this rheostat overcomes ATPase contamination and improves isobutanol production from glucose in a cell-free system. Synthetic biochemistry seeks to engineer complex...

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Veröffentlicht in:	Nature chemical biology 2017-09, Vol.13 (9), p.938-942
Hauptverfasser:	Opgenorth, Paul H, Korman, Tyler P, Iancu, Liviu, Bowie, James U
Format:	Artikel
Sprache:	eng
Schlagworte:	631/114/2390 631/553/318 631/92/1643 631/92/607 Adenosine triphosphatase Adenosine Triphosphate - metabolism Biochemical Engineering Biochemistry Biochemistry & Molecular Biology Bioorganic Chemistry Butanols - metabolism Cell Biology Cell-Free System Chemical synthesis Chemistry Chemistry/Food Science Contamination Glucose Isobutanol Metabolic Engineering Metabolic pathways Models, Molecular Molecular biology Regulatory mechanisms (biology) Signal Transduction System effectiveness
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