Use of small-angle X-ray scattering to resolve intracellular structure changes of Escherichia coli cells induced by antibiotic treatment
The application of small‐angle X‐ray scattering (SAXS) to whole Escherichia coli cells is challenging owing to the variety of internal constituents. To resolve their contributions, the outer shape was captured by ultra‐small‐angle X‐ray scattering and combined with the internal structure resolved by...
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| Veröffentlicht in: | Journal of applied crystallography 2016-12, Vol.49 (6), p.2210-2216 |
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| container_title | Journal of applied crystallography |
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| creator | von Gundlach, A. R. Garamus, V. M. Willey, T. M. Ilavsky, J. Hilpert, K. Rosenhahn, A. |
| description | The application of small‐angle X‐ray scattering (SAXS) to whole Escherichia coli cells is challenging owing to the variety of internal constituents. To resolve their contributions, the outer shape was captured by ultra‐small‐angle X‐ray scattering and combined with the internal structure resolved by SAXS. Building on these data, a model for the major structural components of E. coli was developed. It was possible to deduce information on the occupied volume, occurrence and average size of the most important intracellular constituents: ribosomes, DNA and proteins. E. coli was studied after treatment with three different antibiotic agents (chloramphenicol, tetracycline and rifampicin) and the impact on the intracellular constituents was monitored.
A combination of small‐ and ultra‐small‐angle X‐ray scattering enabled the resolution of important intracellular constituents in Escherichia coli (ribosomes, DNA and proteins). The impact of treatment with three antibiotic agents was monitored. |
| doi_str_mv | 10.1107/S1600576716018562 |
| format | Article |
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A combination of small‐ and ultra‐small‐angle X‐ray scattering enabled the resolution of important intracellular constituents in Escherichia coli (ribosomes, DNA and proteins). The impact of treatment with three antibiotic agents was monitored.</description><identifier>ISSN: 1600-5767</identifier><identifier>ISSN: 0021-8898</identifier><identifier>EISSN: 1600-5767</identifier><identifier>DOI: 10.1107/S1600576716018562</identifier><identifier>PMID: 27980516</identifier><language>eng</language><publisher>5 Abbey Square, Chester, Cheshire CH1 2HU, England: International Union of Crystallography</publisher><subject>60 APPLIED LIFE SCIENCES ; Antibiotics ; Constituents ; Crystallography ; Deoxyribonucleic acid ; E coli ; Escherichia coli ; Escherichia coli ultrastructure ; Genes ; Monitoring ; NANOSCIENCE AND NANOTECHNOLOGY ; Proteins ; SAXS ; Scattering ; Small angle X ray scattering ; TEM ; transmission electron microscopy ; ultra-small-angle X-ray scattering ; USAXS ; X-rays</subject><ispartof>Journal of applied crystallography, 2016-12, Vol.49 (6), p.2210-2216</ispartof><rights>A. R. von Gundlach et al. 2016</rights><rights>A. R. von Gundlach et al. 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5538-f0fc6997713bbe8b2d7b8a540dd9ef04c0613eadb109317ed0e0718be3b35ad93</citedby><cites>FETCH-LOGICAL-c5538-f0fc6997713bbe8b2d7b8a540dd9ef04c0613eadb109317ed0e0718be3b35ad93</cites><orcidid>0000-0003-1982-8900 ; 0000-0001-9393-7190</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1107%2FS1600576716018562$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1107%2FS1600576716018562$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27980516$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/servlets/purl/1459123$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>von Gundlach, A. R.</creatorcontrib><creatorcontrib>Garamus, V. M.</creatorcontrib><creatorcontrib>Willey, T. M.</creatorcontrib><creatorcontrib>Ilavsky, J.</creatorcontrib><creatorcontrib>Hilpert, K.</creatorcontrib><creatorcontrib>Rosenhahn, A.</creatorcontrib><creatorcontrib>Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)</creatorcontrib><creatorcontrib>Argonne National Lab. (ANL), Argonne, IL (United States)</creatorcontrib><title>Use of small-angle X-ray scattering to resolve intracellular structure changes of Escherichia coli cells induced by antibiotic treatment</title><title>Journal of applied crystallography</title><addtitle>J. Appl. Cryst</addtitle><description>The application of small‐angle X‐ray scattering (SAXS) to whole Escherichia coli cells is challenging owing to the variety of internal constituents. To resolve their contributions, the outer shape was captured by ultra‐small‐angle X‐ray scattering and combined with the internal structure resolved by SAXS. Building on these data, a model for the major structural components of E. coli was developed. It was possible to deduce information on the occupied volume, occurrence and average size of the most important intracellular constituents: ribosomes, DNA and proteins. E. coli was studied after treatment with three different antibiotic agents (chloramphenicol, tetracycline and rifampicin) and the impact on the intracellular constituents was monitored.
A combination of small‐ and ultra‐small‐angle X‐ray scattering enabled the resolution of important intracellular constituents in Escherichia coli (ribosomes, DNA and proteins). The impact of treatment with three antibiotic agents was monitored.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Antibiotics</subject><subject>Constituents</subject><subject>Crystallography</subject><subject>Deoxyribonucleic acid</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Escherichia coli ultrastructure</subject><subject>Genes</subject><subject>Monitoring</subject><subject>NANOSCIENCE AND NANOTECHNOLOGY</subject><subject>Proteins</subject><subject>SAXS</subject><subject>Scattering</subject><subject>Small angle X ray scattering</subject><subject>TEM</subject><subject>transmission electron microscopy</subject><subject>ultra-small-angle X-ray scattering</subject><subject>USAXS</subject><subject>X-rays</subject><issn>1600-5767</issn><issn>0021-8898</issn><issn>1600-5767</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><recordid>eNqNksFuFSEUhidGY2v1AdwYohs3ozAMMCzNTW2tjSbapsYNAeZML5U7tMBo7xv42DKZ2hhd2NUhJ9_3J5z8VfWU4FeEYPH6M-EYM8FFmaRjvLlX7c6ret7d_-O9Uz1K6QJjwkXTPKx2GiE7zAjfrX6eJkBhQGmjva_1eO4Bfamj3qJkdc4Q3XiOckARUvDfAbkxR23B-8nriFKOk81TBGTXxYU0R-0nuy6eXTuNbPAOzXgqZj9Z6JHZIj1mZ1zIzqIcQecNjPlx9WDQPsGTm7lXnb7dP1kd1scfD96t3hzXljHa1QMeLJdSCEKNgc40vTCdZi3uewkDbi3mhILuDcGSEgE9BixIZ4AaynQv6V71fMkNKTuVrMtg1zaMI9isSMskaWiBXi7QZQxXE6SsNi7N39AjhCkp0vGWtVISeReUNgQz3t4BZQ3vBGeioC_-Qi_CFMdymEK1HDeipaRQZKFsDClFGNRldBsdt4pgNTdE_dOQ4jy7SZ7MBvpb43clCiAX4IfzsP1_ojpafWqO3jOMu-LWi-tShutbV8dvigsqmDr7cKC-ypXALTtTJ_QXSS_VaA</recordid><startdate>20161201</startdate><enddate>20161201</enddate><creator>von Gundlach, A. R.</creator><creator>Garamus, V. M.</creator><creator>Willey, T. M.</creator><creator>Ilavsky, J.</creator><creator>Hilpert, K.</creator><creator>Rosenhahn, A.</creator><general>International Union of Crystallography</general><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>24P</scope><scope>WIN</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope><scope>OIOZB</scope><scope>OTOTI</scope><orcidid>https://orcid.org/0000-0003-1982-8900</orcidid><orcidid>https://orcid.org/0000-0001-9393-7190</orcidid></search><sort><creationdate>20161201</creationdate><title>Use of small-angle X-ray scattering to resolve intracellular structure changes of Escherichia coli cells induced by antibiotic treatment</title><author>von Gundlach, A. R. ; Garamus, V. M. ; Willey, T. 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R.</creatorcontrib><creatorcontrib>Garamus, V. M.</creatorcontrib><creatorcontrib>Willey, T. M.</creatorcontrib><creatorcontrib>Ilavsky, J.</creatorcontrib><creatorcontrib>Hilpert, K.</creatorcontrib><creatorcontrib>Rosenhahn, A.</creatorcontrib><creatorcontrib>Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)</creatorcontrib><creatorcontrib>Argonne National Lab. 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A combination of small‐ and ultra‐small‐angle X‐ray scattering enabled the resolution of important intracellular constituents in Escherichia coli (ribosomes, DNA and proteins). The impact of treatment with three antibiotic agents was monitored.</abstract><cop>5 Abbey Square, Chester, Cheshire CH1 2HU, England</cop><pub>International Union of Crystallography</pub><pmid>27980516</pmid><doi>10.1107/S1600576716018562</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-1982-8900</orcidid><orcidid>https://orcid.org/0000-0001-9393-7190</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of applied crystallography, 2016-12, Vol.49 (6), p.2210-2216 |
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| source | Access via Wiley Online Library; Alma/SFX Local Collection |
| subjects | 60 APPLIED LIFE SCIENCES Antibiotics Constituents Crystallography Deoxyribonucleic acid E coli Escherichia coli Escherichia coli ultrastructure Genes Monitoring NANOSCIENCE AND NANOTECHNOLOGY Proteins SAXS Scattering Small angle X ray scattering TEM transmission electron microscopy ultra-small-angle X-ray scattering USAXS X-rays |
| title | Use of small-angle X-ray scattering to resolve intracellular structure changes of Escherichia coli cells induced by antibiotic treatment |
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