Fluctuation localization imaging-based fluorescence in situ hybridization (fliFISH) for accurate detection and counting of RNA copies in single cells

Here, quantitative gene expression analysis in intact single cells can be achieved using single molecule-based fluorescence in situ hybridization (smFISH). This approach relies on fluorescence intensity to distinguish between true signals, emitted from an RNA copy hybridized with multiple oligonucle...

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Veröffentlicht in:	Nucleic acids research 2017-10, Vol.46 (2)
Hauptverfasser:	Cui, Yi, Hu, Dehong, Markillie, Lye Meng, Chrisler, William B., Gaffrey, Matthew J., Ansong, Charles, Sussel, Lori, Orr, Galya
Format:	Artikel
Sprache:	eng
Schlagworte:	BASIC BIOLOGICAL SCIENCES
Online-Zugang:	Volltext
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