Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome

Here, we report the successful design, construction, and characterization of a 770-kilobase synthetic yeast chromosome II (synII). Our study incorporates characterization at multiple levels-including phenomics, transcriptomics, proteomics, chromosome segregation, and replication analysis-to provide...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 2017-03, Vol.355 (6329), p.1047-1047
Hauptverfasser:	Shen, Yue, Wang, Yun, Chen, Tai, Gao, Feng, Gong, Jianhui, Abramczyk, Dariusz, Walker, Roy, Zhao, Hongcui, Chen, Shihong, Liu, Wei, Luo, Yisha, Müller, Carolin A., Paul-Dubois-Taine, Adrien, Alver, Bonnie, Stracquadanio, Giovanni, Mitchell, Leslie A., Luo, Zhouqing, Fan, Yanqun, Zhou, Baojin, Wen, Bo, Tan, Fengji, Wang, Yujia, Zi, Jin, Xie, Zexiong, Li, Bingzhi, Yang, Kun, Richardson, Sarah M., Jiang, Hui, French, Christopher E., Nieduszynski, Conrad A., Koszul, Romain, Marston, Adele L., Yuan, Yingjin, Wang, Jian, Bader, Joel S., Dai, Junbiao, Boeke, Jef D., Xu, Xun, Cai, Yizhi, Yang, Huanming
Format:	Artikel
Sprache:	eng
Schlagworte:	Anaphase Assaying Biological activity Carbon sources Cell cycle Cellular communication Chromosome 2 Chromosome Segregation Chromosomes Chromosomes, Artificial, Yeast - chemistry Chromosomes, Artificial, Yeast - genetics Chromosomes, Artificial, Yeast - physiology Clonal deletion Colonies Complementation Complexity Computer networks Construction Copy number Culture Media - chemistry Debugging Defects Deletion Deoxyribonucleic acid Design DNA DNA biosynthesis DNA Replication Evolution Functional analysis Gene expression Gene regulation Gene sequencing Genes Genome, Fungal Genomes Glycerol Glycerols High temperature Homologous recombination Homology In vivo methods and tests Integration Iterative methods Life Sciences Literary Devices Logical Thinking Meiosis Metabolomics Metaphase Origins Osmolarity Phenotyping Proteomics Replication RESEARCH ARTICLE SUMMARY Ribonucleic acid RNA Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development Segregations Sequence Analysis, DNA Strategy Synthetic Biology Temperature effects Transcriptome Translation tRNA Yeast
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