Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome
Here, we report the successful design, construction, and characterization of a 770-kilobase synthetic yeast chromosome II (synII). Our study incorporates characterization at multiple levels-including phenomics, transcriptomics, proteomics, chromosome segregation, and replication analysis-to provide...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2017-03, Vol.355 (6329), p.1047-1047 |
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| creator | Shen, Yue Wang, Yun Chen, Tai Gao, Feng Gong, Jianhui Abramczyk, Dariusz Walker, Roy Zhao, Hongcui Chen, Shihong Liu, Wei Luo, Yisha Müller, Carolin A. Paul-Dubois-Taine, Adrien Alver, Bonnie Stracquadanio, Giovanni Mitchell, Leslie A. Luo, Zhouqing Fan, Yanqun Zhou, Baojin Wen, Bo Tan, Fengji Wang, Yujia Zi, Jin Xie, Zexiong Li, Bingzhi Yang, Kun Richardson, Sarah M. Jiang, Hui French, Christopher E. Nieduszynski, Conrad A. Koszul, Romain Marston, Adele L. Yuan, Yingjin Wang, Jian Bader, Joel S. Dai, Junbiao Boeke, Jef D. Xu, Xun Cai, Yizhi Yang, Huanming |
| description | Here, we report the successful design, construction, and characterization of a 770-kilobase synthetic yeast chromosome II (synII). Our study incorporates characterization at multiple levels-including phenomics, transcriptomics, proteomics, chromosome segregation, and replication analysis-to provide a thorough and comprehensive analysis of a synthetic chromosome. Our Trans-Omics analyses reveal a modest but potentially relevant pervasive up-regulation of translational machinery observed in synII, mainly caused by the deletion of 13 transfer RNAs. By both complementation assays and SCRaMbLE (synthetic chromosome rearrangement and modification by
-mediated evolution), we targeted and debugged the origin of a growth defect at 37°C in glycerol medium, which is related to misregulation of the high-osmolarity glycerol response. Despite the subtle differences, the synII strain shows highly consistent biological processes comparable to the native strain. |
| doi_str_mv | 10.1126/science.aaf4791 |
| format | Article |
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-mediated evolution), we targeted and debugged the origin of a growth defect at 37°C in glycerol medium, which is related to misregulation of the high-osmolarity glycerol response. Despite the subtle differences, the synII strain shows highly consistent biological processes comparable to the native strain.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.aaf4791</identifier><identifier>PMID: 28280153</identifier><language>eng</language><publisher>United States: American Association for the Advancement of Science</publisher><subject>Anaphase ; Assaying ; Biological activity ; Carbon sources ; Cell cycle ; Cellular communication ; Chromosome 2 ; Chromosome Segregation ; Chromosomes ; Chromosomes, Artificial, Yeast - chemistry ; Chromosomes, Artificial, Yeast - genetics ; Chromosomes, Artificial, Yeast - physiology ; Clonal deletion ; Colonies ; Complementation ; Complexity ; Computer networks ; Construction ; Copy number ; Culture Media - chemistry ; Debugging ; Defects ; Deletion ; Deoxyribonucleic acid ; Design ; DNA ; DNA biosynthesis ; DNA Replication ; Evolution ; Functional analysis ; Gene expression ; Gene regulation ; Gene sequencing ; Genes ; Genome, Fungal ; Genomes ; Glycerol ; Glycerols ; High temperature ; Homologous recombination ; Homology ; In vivo methods and tests ; Integration ; Iterative methods ; Life Sciences ; Literary Devices ; Logical Thinking ; Meiosis ; Metabolomics ; Metaphase ; Origins ; Osmolarity ; Phenotyping ; Proteomics ; Replication ; RESEARCH ARTICLE SUMMARY ; Ribonucleic acid ; RNA ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - growth & development ; Segregations ; Sequence Analysis, DNA ; Strategy ; Synthetic Biology ; Temperature effects ; Transcriptome ; Translation ; tRNA ; Yeast</subject><ispartof>Science (American Association for the Advancement of Science), 2017-03, Vol.355 (6329), p.1047-1047</ispartof><rights>Copyright © 2017 American Association for the Advancement of Science</rights><rights>Copyright © 2017, American Association for the Advancement of Science.</rights><rights>Copyright © 2017, American Association for the Advancement of Science</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c585t-e82a0acd2e4c1b83c4cf9f62d801b38d474cc76c0596d7f4584ca271dcf130e13</citedby><cites>FETCH-LOGICAL-c585t-e82a0acd2e4c1b83c4cf9f62d801b38d474cc76c0596d7f4584ca271dcf130e13</cites><orcidid>0000-0001-7869-9817 ; 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0000000235969407 ; 0000000252994700 ; 0000000198193645 ; 0000000228695620 ; 0000000239109260 ; 0000000262149009</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/24918495$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/24918495$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,799,881,2871,2872,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28280153$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://pasteur.hal.science/pasteur-02868099$$DView record in HAL$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/1435471$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Shen, Yue</creatorcontrib><creatorcontrib>Wang, Yun</creatorcontrib><creatorcontrib>Chen, Tai</creatorcontrib><creatorcontrib>Gao, Feng</creatorcontrib><creatorcontrib>Gong, Jianhui</creatorcontrib><creatorcontrib>Abramczyk, Dariusz</creatorcontrib><creatorcontrib>Walker, Roy</creatorcontrib><creatorcontrib>Zhao, Hongcui</creatorcontrib><creatorcontrib>Chen, Shihong</creatorcontrib><creatorcontrib>Liu, Wei</creatorcontrib><creatorcontrib>Luo, Yisha</creatorcontrib><creatorcontrib>Müller, Carolin A.</creatorcontrib><creatorcontrib>Paul-Dubois-Taine, Adrien</creatorcontrib><creatorcontrib>Alver, Bonnie</creatorcontrib><creatorcontrib>Stracquadanio, Giovanni</creatorcontrib><creatorcontrib>Mitchell, Leslie A.</creatorcontrib><creatorcontrib>Luo, Zhouqing</creatorcontrib><creatorcontrib>Fan, Yanqun</creatorcontrib><creatorcontrib>Zhou, Baojin</creatorcontrib><creatorcontrib>Wen, Bo</creatorcontrib><creatorcontrib>Tan, Fengji</creatorcontrib><creatorcontrib>Wang, Yujia</creatorcontrib><creatorcontrib>Zi, Jin</creatorcontrib><creatorcontrib>Xie, Zexiong</creatorcontrib><creatorcontrib>Li, Bingzhi</creatorcontrib><creatorcontrib>Yang, Kun</creatorcontrib><creatorcontrib>Richardson, Sarah M.</creatorcontrib><creatorcontrib>Jiang, Hui</creatorcontrib><creatorcontrib>French, Christopher E.</creatorcontrib><creatorcontrib>Nieduszynski, Conrad A.</creatorcontrib><creatorcontrib>Koszul, Romain</creatorcontrib><creatorcontrib>Marston, Adele L.</creatorcontrib><creatorcontrib>Yuan, Yingjin</creatorcontrib><creatorcontrib>Wang, Jian</creatorcontrib><creatorcontrib>Bader, Joel S.</creatorcontrib><creatorcontrib>Dai, Junbiao</creatorcontrib><creatorcontrib>Boeke, Jef D.</creatorcontrib><creatorcontrib>Xu, Xun</creatorcontrib><creatorcontrib>Cai, Yizhi</creatorcontrib><creatorcontrib>Yang, Huanming</creatorcontrib><title>Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Here, we report the successful design, construction, and characterization of a 770-kilobase synthetic yeast chromosome II (synII). Our study incorporates characterization at multiple levels-including phenomics, transcriptomics, proteomics, chromosome segregation, and replication analysis-to provide a thorough and comprehensive analysis of a synthetic chromosome. Our Trans-Omics analyses reveal a modest but potentially relevant pervasive up-regulation of translational machinery observed in synII, mainly caused by the deletion of 13 transfer RNAs. By both complementation assays and SCRaMbLE (synthetic chromosome rearrangement and modification by
-mediated evolution), we targeted and debugged the origin of a growth defect at 37°C in glycerol medium, which is related to misregulation of the high-osmolarity glycerol response. Despite the subtle differences, the synII strain shows highly consistent biological processes comparable to the native strain.</description><subject>Anaphase</subject><subject>Assaying</subject><subject>Biological activity</subject><subject>Carbon sources</subject><subject>Cell cycle</subject><subject>Cellular communication</subject><subject>Chromosome 2</subject><subject>Chromosome Segregation</subject><subject>Chromosomes</subject><subject>Chromosomes, Artificial, Yeast - chemistry</subject><subject>Chromosomes, Artificial, Yeast - genetics</subject><subject>Chromosomes, Artificial, Yeast - physiology</subject><subject>Clonal deletion</subject><subject>Colonies</subject><subject>Complementation</subject><subject>Complexity</subject><subject>Computer networks</subject><subject>Construction</subject><subject>Copy number</subject><subject>Culture Media - chemistry</subject><subject>Debugging</subject><subject>Defects</subject><subject>Deletion</subject><subject>Deoxyribonucleic acid</subject><subject>Design</subject><subject>DNA</subject><subject>DNA biosynthesis</subject><subject>DNA Replication</subject><subject>Evolution</subject><subject>Functional analysis</subject><subject>Gene expression</subject><subject>Gene regulation</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genome, Fungal</subject><subject>Genomes</subject><subject>Glycerol</subject><subject>Glycerols</subject><subject>High temperature</subject><subject>Homologous recombination</subject><subject>Homology</subject><subject>In vivo methods and tests</subject><subject>Integration</subject><subject>Iterative methods</subject><subject>Life Sciences</subject><subject>Literary Devices</subject><subject>Logical Thinking</subject><subject>Meiosis</subject><subject>Metabolomics</subject><subject>Metaphase</subject><subject>Origins</subject><subject>Osmolarity</subject><subject>Phenotyping</subject><subject>Proteomics</subject><subject>Replication</subject><subject>RESEARCH ARTICLE SUMMARY</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - growth & development</subject><subject>Segregations</subject><subject>Sequence Analysis, DNA</subject><subject>Strategy</subject><subject>Synthetic Biology</subject><subject>Temperature effects</subject><subject>Transcriptome</subject><subject>Translation</subject><subject>tRNA</subject><subject>Yeast</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNks1v1DAQxS0EokvLmRMoggsH0vozto9V-ehKK_VSzpZ3MtFmSeIldirtf4_TLEXi1IsteX5-ejPzCHnH6CVjvLqK0OIAeOl9I7VlL8iKUatKy6l4SVaUiqo0VKsz8ibGPaW5ZsVrcsYNN5QpsSKbr4iHopkGSG0YfFf4fBxjG4vQFPE4rNdfCl9oTctfbRe2PuL8mnaYWiiO6GMqYDeGPsTQ4wV51fgu4tvTfU5-fv92f3Nbbu5-rG-uNyUoo1KJhnvqoeYogW2NAAmNbSpeZ09bYWqpJYCugCpb1bqRykjwXLMaGiYoMnFOPi66IabW5RkkhB2EYUBIjkmhpJ6hcoF2vnOHse39eHTBt-72euMO2ThOo6PcVIZa-zDznxf-MIbfE8bk-jYCdp0fMEzRMWOFsYxp-wzUMF3JLP0MVFdq3tNs4NN_6D5MY17Ho6DIo5MVz9TVQsEYYhyxeeqNUTdnwp0y4U6ZyD8-nHSnbY_1E_83BBl4vwD7mML4ry4tM9Iq8QcEt7rU</recordid><startdate>20170310</startdate><enddate>20170310</enddate><creator>Shen, Yue</creator><creator>Wang, Yun</creator><creator>Chen, 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functional analysis of synII, a 770-kilobase synthetic yeast chromosome</title><author>Shen, Yue ; Wang, Yun ; Chen, Tai ; Gao, Feng ; Gong, Jianhui ; Abramczyk, Dariusz ; Walker, Roy ; Zhao, Hongcui ; Chen, Shihong ; Liu, Wei ; Luo, Yisha ; Müller, Carolin A. ; Paul-Dubois-Taine, Adrien ; Alver, Bonnie ; Stracquadanio, Giovanni ; Mitchell, Leslie A. ; Luo, Zhouqing ; Fan, Yanqun ; Zhou, Baojin ; Wen, Bo ; Tan, Fengji ; Wang, Yujia ; Zi, Jin ; Xie, Zexiong ; Li, Bingzhi ; Yang, Kun ; Richardson, Sarah M. ; Jiang, Hui ; French, Christopher E. ; Nieduszynski, Conrad A. ; Koszul, Romain ; Marston, Adele L. ; Yuan, Yingjin ; Wang, Jian ; Bader, Joel S. ; Dai, Junbiao ; Boeke, Jef D. ; Xu, Xun ; Cai, Yizhi ; Yang, Huanming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c585t-e82a0acd2e4c1b83c4cf9f62d801b38d474cc76c0596d7f4584ca271dcf130e13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Anaphase</topic><topic>Assaying</topic><topic>Biological activity</topic><topic>Carbon sources</topic><topic>Cell cycle</topic><topic>Cellular communication</topic><topic>Chromosome 2</topic><topic>Chromosome Segregation</topic><topic>Chromosomes</topic><topic>Chromosomes, Artificial, Yeast - chemistry</topic><topic>Chromosomes, Artificial, Yeast - genetics</topic><topic>Chromosomes, Artificial, Yeast - physiology</topic><topic>Clonal deletion</topic><topic>Colonies</topic><topic>Complementation</topic><topic>Complexity</topic><topic>Computer networks</topic><topic>Construction</topic><topic>Copy number</topic><topic>Culture Media - chemistry</topic><topic>Debugging</topic><topic>Defects</topic><topic>Deletion</topic><topic>Deoxyribonucleic acid</topic><topic>Design</topic><topic>DNA</topic><topic>DNA biosynthesis</topic><topic>DNA Replication</topic><topic>Evolution</topic><topic>Functional analysis</topic><topic>Gene expression</topic><topic>Gene regulation</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Genome, Fungal</topic><topic>Genomes</topic><topic>Glycerol</topic><topic>Glycerols</topic><topic>High temperature</topic><topic>Homologous recombination</topic><topic>Homology</topic><topic>In vivo methods and tests</topic><topic>Integration</topic><topic>Iterative methods</topic><topic>Life Sciences</topic><topic>Literary Devices</topic><topic>Logical Thinking</topic><topic>Meiosis</topic><topic>Metabolomics</topic><topic>Metaphase</topic><topic>Origins</topic><topic>Osmolarity</topic><topic>Phenotyping</topic><topic>Proteomics</topic><topic>Replication</topic><topic>RESEARCH ARTICLE SUMMARY</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - growth & development</topic><topic>Segregations</topic><topic>Sequence Analysis, DNA</topic><topic>Strategy</topic><topic>Synthetic Biology</topic><topic>Temperature effects</topic><topic>Transcriptome</topic><topic>Translation</topic><topic>tRNA</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shen, Yue</creatorcontrib><creatorcontrib>Wang, Yun</creatorcontrib><creatorcontrib>Chen, Tai</creatorcontrib><creatorcontrib>Gao, Feng</creatorcontrib><creatorcontrib>Gong, Jianhui</creatorcontrib><creatorcontrib>Abramczyk, Dariusz</creatorcontrib><creatorcontrib>Walker, Roy</creatorcontrib><creatorcontrib>Zhao, Hongcui</creatorcontrib><creatorcontrib>Chen, Shihong</creatorcontrib><creatorcontrib>Liu, Wei</creatorcontrib><creatorcontrib>Luo, Yisha</creatorcontrib><creatorcontrib>Müller, Carolin A.</creatorcontrib><creatorcontrib>Paul-Dubois-Taine, Adrien</creatorcontrib><creatorcontrib>Alver, Bonnie</creatorcontrib><creatorcontrib>Stracquadanio, Giovanni</creatorcontrib><creatorcontrib>Mitchell, Leslie A.</creatorcontrib><creatorcontrib>Luo, Zhouqing</creatorcontrib><creatorcontrib>Fan, Yanqun</creatorcontrib><creatorcontrib>Zhou, Baojin</creatorcontrib><creatorcontrib>Wen, Bo</creatorcontrib><creatorcontrib>Tan, Fengji</creatorcontrib><creatorcontrib>Wang, Yujia</creatorcontrib><creatorcontrib>Zi, Jin</creatorcontrib><creatorcontrib>Xie, Zexiong</creatorcontrib><creatorcontrib>Li, Bingzhi</creatorcontrib><creatorcontrib>Yang, Kun</creatorcontrib><creatorcontrib>Richardson, Sarah M.</creatorcontrib><creatorcontrib>Jiang, Hui</creatorcontrib><creatorcontrib>French, Christopher E.</creatorcontrib><creatorcontrib>Nieduszynski, Conrad A.</creatorcontrib><creatorcontrib>Koszul, Romain</creatorcontrib><creatorcontrib>Marston, Adele L.</creatorcontrib><creatorcontrib>Yuan, Yingjin</creatorcontrib><creatorcontrib>Wang, Jian</creatorcontrib><creatorcontrib>Bader, Joel S.</creatorcontrib><creatorcontrib>Dai, Junbiao</creatorcontrib><creatorcontrib>Boeke, Jef D.</creatorcontrib><creatorcontrib>Xu, Xun</creatorcontrib><creatorcontrib>Cai, Yizhi</creatorcontrib><creatorcontrib>Yang, Huanming</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems 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Association for the Advancement of Science)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shen, Yue</au><au>Wang, Yun</au><au>Chen, Tai</au><au>Gao, Feng</au><au>Gong, Jianhui</au><au>Abramczyk, Dariusz</au><au>Walker, Roy</au><au>Zhao, Hongcui</au><au>Chen, Shihong</au><au>Liu, Wei</au><au>Luo, Yisha</au><au>Müller, Carolin A.</au><au>Paul-Dubois-Taine, Adrien</au><au>Alver, Bonnie</au><au>Stracquadanio, Giovanni</au><au>Mitchell, Leslie A.</au><au>Luo, Zhouqing</au><au>Fan, Yanqun</au><au>Zhou, Baojin</au><au>Wen, Bo</au><au>Tan, Fengji</au><au>Wang, Yujia</au><au>Zi, Jin</au><au>Xie, Zexiong</au><au>Li, Bingzhi</au><au>Yang, Kun</au><au>Richardson, Sarah M.</au><au>Jiang, Hui</au><au>French, Christopher E.</au><au>Nieduszynski, Conrad A.</au><au>Koszul, Romain</au><au>Marston, Adele L.</au><au>Yuan, Yingjin</au><au>Wang, Jian</au><au>Bader, Joel S.</au><au>Dai, Junbiao</au><au>Boeke, Jef D.</au><au>Xu, Xun</au><au>Cai, Yizhi</au><au>Yang, Huanming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>2017-03-10</date><risdate>2017</risdate><volume>355</volume><issue>6329</issue><spage>1047</spage><epage>1047</epage><pages>1047-1047</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><abstract>Here, we report the successful design, construction, and characterization of a 770-kilobase synthetic yeast chromosome II (synII). Our study incorporates characterization at multiple levels-including phenomics, transcriptomics, proteomics, chromosome segregation, and replication analysis-to provide a thorough and comprehensive analysis of a synthetic chromosome. Our Trans-Omics analyses reveal a modest but potentially relevant pervasive up-regulation of translational machinery observed in synII, mainly caused by the deletion of 13 transfer RNAs. By both complementation assays and SCRaMbLE (synthetic chromosome rearrangement and modification by
-mediated evolution), we targeted and debugged the origin of a growth defect at 37°C in glycerol medium, which is related to misregulation of the high-osmolarity glycerol response. Despite the subtle differences, the synII strain shows highly consistent biological processes comparable to the native strain.</abstract><cop>United States</cop><pub>American Association for the Advancement of Science</pub><pmid>28280153</pmid><doi>10.1126/science.aaf4791</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-7869-9817</orcidid><orcidid>https://orcid.org/0000-0003-1703-3012</orcidid><orcidid>https://orcid.org/0000-0003-0553-0089</orcidid><orcidid>https://orcid.org/0000-0001-5872-3269</orcidid><orcidid>https://orcid.org/0000-0002-9820-9421</orcidid><orcidid>https://orcid.org/0000-0003-0960-4591</orcidid><orcidid>https://orcid.org/0000-0003-1663-2865</orcidid><orcidid>https://orcid.org/0000-0002-4228-7527</orcidid><orcidid>https://orcid.org/0000-0002-3276-7295</orcidid><orcidid>https://orcid.org/0000-0003-2001-076X</orcidid><orcidid>https://orcid.org/0000-0001-9819-3645</orcidid><orcidid>https://orcid.org/0000-0002-3086-1173</orcidid><orcidid>https://orcid.org/0000-0002-7422-5582</orcidid><orcidid>https://orcid.org/0000-0002-2860-1321</orcidid><orcidid>https://orcid.org/0000-0002-3596-9407</orcidid><orcidid>https://orcid.org/0000-0001-5426-5176</orcidid><orcidid>https://orcid.org/0000-0003-4121-3048</orcidid><orcidid>https://orcid.org/0000-0001-5322-4946</orcidid><orcidid>https://orcid.org/0000-0002-3910-9260</orcidid><orcidid>https://orcid.org/0000-0002-1060-8082</orcidid><orcidid>https://orcid.org/0000-0002-8698-464X</orcidid><orcidid>https://orcid.org/0000-0002-6214-9009</orcidid><orcidid>https://orcid.org/0000-0002-1326-9901</orcidid><orcidid>https://orcid.org/0000-0002-2869-5620</orcidid><orcidid>https://orcid.org/0000-0002-6020-4625</orcidid><orcidid>https://orcid.org/0000-0002-5299-4700</orcidid><orcidid>https://orcid.org/0000-0002-4989-3992</orcidid><orcidid>https://orcid.org/0000-0002-5338-5173</orcidid><orcidid>https://orcid.org/0000-0003-0480-2097</orcidid><orcidid>https://orcid.org/0000-0002-0809-2371</orcidid><orcidid>https://orcid.org/0000000153224946</orcidid><orcidid>https://orcid.org/0000000158723269</orcidid><orcidid>https://orcid.org/0000000228601321</orcidid><orcidid>https://orcid.org/0000000232767295</orcidid><orcidid>https://orcid.org/0000000274225582</orcidid><orcidid>https://orcid.org/0000000213269901</orcidid><orcidid>https://orcid.org/0000000178699817</orcidid><orcidid>https://orcid.org/0000000230861173</orcidid><orcidid>https://orcid.org/0000000154265176</orcidid><orcidid>https://orcid.org/0000000260204625</orcidid><orcidid>https://orcid.org/0000000309604591</orcidid><orcidid>https://orcid.org/0000000210608082</orcidid><orcidid>https://orcid.org/0000000305530089</orcidid><orcidid>https://orcid.org/0000000298209421</orcidid><orcidid>https://orcid.org/000000032001076X</orcidid><orcidid>https://orcid.org/0000000242287527</orcidid><orcidid>https://orcid.org/0000000317033012</orcidid><orcidid>https://orcid.org/0000000341213048</orcidid><orcidid>https://orcid.org/0000000316632865</orcidid><orcidid>https://orcid.org/000000028698464X</orcidid><orcidid>https://orcid.org/0000000235969407</orcidid><orcidid>https://orcid.org/0000000252994700</orcidid><orcidid>https://orcid.org/0000000198193645</orcidid><orcidid>https://orcid.org/0000000228695620</orcidid><orcidid>https://orcid.org/0000000239109260</orcidid><orcidid>https://orcid.org/0000000262149009</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 2017-03, Vol.355 (6329), p.1047-1047 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_osti_scitechconnect_1435471 |
| source | Jstor Complete Legacy; MEDLINE; Science Magazine |
| subjects | Anaphase Assaying Biological activity Carbon sources Cell cycle Cellular communication Chromosome 2 Chromosome Segregation Chromosomes Chromosomes, Artificial, Yeast - chemistry Chromosomes, Artificial, Yeast - genetics Chromosomes, Artificial, Yeast - physiology Clonal deletion Colonies Complementation Complexity Computer networks Construction Copy number Culture Media - chemistry Debugging Defects Deletion Deoxyribonucleic acid Design DNA DNA biosynthesis DNA Replication Evolution Functional analysis Gene expression Gene regulation Gene sequencing Genes Genome, Fungal Genomes Glycerol Glycerols High temperature Homologous recombination Homology In vivo methods and tests Integration Iterative methods Life Sciences Literary Devices Logical Thinking Meiosis Metabolomics Metaphase Origins Osmolarity Phenotyping Proteomics Replication RESEARCH ARTICLE SUMMARY Ribonucleic acid RNA Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development Segregations Sequence Analysis, DNA Strategy Synthetic Biology Temperature effects Transcriptome Translation tRNA Yeast |
| title | Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome |
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