Engineering a novel biosynthetic pathway in Escherichia coli for production of renewable ethylene glycol
ABSTRACT Ethylene glycol (EG) is an important commodity chemical with broad industrial applications. It is presently produced from petroleum or natural gas feedstocks in processes requiring consumption of significant quantities of non‐renewable resources. Here, we report a novel pathway for biosynth...
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| Veröffentlicht in: | Biotechnology and bioengineering 2016-02, Vol.113 (2), p.376-383 |
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| creator | Pereira, Brian Zhang, Haoran De Mey, Marjan Lim, Chin Giaw Li, Zheng-Jun Stephanopoulos, Gregory |
| description | ABSTRACT
Ethylene glycol (EG) is an important commodity chemical with broad industrial applications. It is presently produced from petroleum or natural gas feedstocks in processes requiring consumption of significant quantities of non‐renewable resources. Here, we report a novel pathway for biosynthesis of EG from the renewable sugar glucose in metabolically engineered Escherichia coli. Serine‐to‐EG conversion was first achieved through a pathway comprising serine decarboxylase, ethanolamine oxidase, and glycolaldehyde reductase. Serine provision in E. coli was then enhanced by overexpression of the serine‐biosynthesis pathway. The integration of these two parts into the complete EG‐biosynthesis pathway in E. coli allowed for production of 4.1 g/L EG at a cumulative yield of 0.14 g‐EG/g‐glucose, establishing a foundation for a promising biotechnology. Biotechnol. Bioeng. 2016;113: 376–383. © 2015 Wiley Periodicals, Inc.
A novel ethylene glycol (EG) biosynthetic pathway was engineered in bacterium in E. coli to achieve de novo production from renewable sugar glucose. The pathway includes the upstream part for serine biosynthesis and the downstream part for serine‐to‐EG conversion. Through metabolically engineering these two parts individually, we achieved production of 4.1 g/L EG at a yield of 0.14 g‐EG/g‐glucose. |
| doi_str_mv | 10.1002/bit.25717 |
| format | Article |
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Ethylene glycol (EG) is an important commodity chemical with broad industrial applications. It is presently produced from petroleum or natural gas feedstocks in processes requiring consumption of significant quantities of non‐renewable resources. Here, we report a novel pathway for biosynthesis of EG from the renewable sugar glucose in metabolically engineered Escherichia coli. Serine‐to‐EG conversion was first achieved through a pathway comprising serine decarboxylase, ethanolamine oxidase, and glycolaldehyde reductase. Serine provision in E. coli was then enhanced by overexpression of the serine‐biosynthesis pathway. The integration of these two parts into the complete EG‐biosynthesis pathway in E. coli allowed for production of 4.1 g/L EG at a cumulative yield of 0.14 g‐EG/g‐glucose, establishing a foundation for a promising biotechnology. Biotechnol. Bioeng. 2016;113: 376–383. © 2015 Wiley Periodicals, Inc.
A novel ethylene glycol (EG) biosynthetic pathway was engineered in bacterium in E. coli to achieve de novo production from renewable sugar glucose. The pathway includes the upstream part for serine biosynthesis and the downstream part for serine‐to‐EG conversion. Through metabolically engineering these two parts individually, we achieved production of 4.1 g/L EG at a yield of 0.14 g‐EG/g‐glucose.</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.25717</identifier><identifier>PMID: 26221864</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Antifreeze solutions ; Biosynthesis ; Biosynthetic Pathways - genetics ; Biotechnology ; Conversion ; E coli ; Escherichia coli ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Ethylene glycol ; Ethylene Glycol - metabolism ; Glucose ; Glucose - metabolism ; metabolic engineering ; Metabolic Engineering - methods ; Metabolism ; Natural gas ; Nonrenewable resources ; Pathways ; renewable ; Renewable resources ; serine ; Serine - metabolism ; Sugar ; Sugars</subject><ispartof>Biotechnology and bioengineering, 2016-02, Vol.113 (2), p.376-383</ispartof><rights>2015 Wiley Periodicals, Inc.</rights><rights>Copyright Wiley Subscription Services, Inc. Feb 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6927-abe4f7c65b6e4a9ccd6a83bf140bc6358066c474cb75ca0a534ee36a6a3667213</citedby><cites>FETCH-LOGICAL-c6927-abe4f7c65b6e4a9ccd6a83bf140bc6358066c474cb75ca0a534ee36a6a3667213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbit.25717$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbit.25717$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26221864$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/1401151$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Pereira, Brian</creatorcontrib><creatorcontrib>Zhang, Haoran</creatorcontrib><creatorcontrib>De Mey, Marjan</creatorcontrib><creatorcontrib>Lim, Chin Giaw</creatorcontrib><creatorcontrib>Li, Zheng-Jun</creatorcontrib><creatorcontrib>Stephanopoulos, Gregory</creatorcontrib><title>Engineering a novel biosynthetic pathway in Escherichia coli for production of renewable ethylene glycol</title><title>Biotechnology and bioengineering</title><addtitle>Biotechnol. Bioeng</addtitle><description>ABSTRACT
Ethylene glycol (EG) is an important commodity chemical with broad industrial applications. It is presently produced from petroleum or natural gas feedstocks in processes requiring consumption of significant quantities of non‐renewable resources. Here, we report a novel pathway for biosynthesis of EG from the renewable sugar glucose in metabolically engineered Escherichia coli. Serine‐to‐EG conversion was first achieved through a pathway comprising serine decarboxylase, ethanolamine oxidase, and glycolaldehyde reductase. Serine provision in E. coli was then enhanced by overexpression of the serine‐biosynthesis pathway. The integration of these two parts into the complete EG‐biosynthesis pathway in E. coli allowed for production of 4.1 g/L EG at a cumulative yield of 0.14 g‐EG/g‐glucose, establishing a foundation for a promising biotechnology. Biotechnol. Bioeng. 2016;113: 376–383. © 2015 Wiley Periodicals, Inc.
A novel ethylene glycol (EG) biosynthetic pathway was engineered in bacterium in E. coli to achieve de novo production from renewable sugar glucose. The pathway includes the upstream part for serine biosynthesis and the downstream part for serine‐to‐EG conversion. Through metabolically engineering these two parts individually, we achieved production of 4.1 g/L EG at a yield of 0.14 g‐EG/g‐glucose.</description><subject>Antifreeze solutions</subject><subject>Biosynthesis</subject><subject>Biosynthetic Pathways - genetics</subject><subject>Biotechnology</subject><subject>Conversion</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>Ethylene glycol</subject><subject>Ethylene Glycol - metabolism</subject><subject>Glucose</subject><subject>Glucose - metabolism</subject><subject>metabolic engineering</subject><subject>Metabolic Engineering - methods</subject><subject>Metabolism</subject><subject>Natural gas</subject><subject>Nonrenewable resources</subject><subject>Pathways</subject><subject>renewable</subject><subject>Renewable resources</subject><subject>serine</subject><subject>Serine - metabolism</subject><subject>Sugar</subject><subject>Sugars</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0cFu1DAQBuAIgei2cOAFkAWXckhrO_E4OUK1XSpVcCn0aDneycYlay-xw5K3x0vaHpAQnKyRvvllzZ9lrxg9Y5Ty88bGMy4kk0-yBaO1zCmv6dNsQSmFvBA1P8qOQ7hLo6wAnmdHHDhnFZSLrFu6jXWIg3UboonzP7AnjfVhcrHDaA3Z6djt9USsI8tguiRNZzUxvrek9QPZDX49mmi9I74lAzrc66ZHgrGb-jSRTT8l_CJ71uo-4Mv79yT7crm8ufiYX39eXV28v84N1FzmusGylQZEA1jq2pg16KpoWlbSxkAhKgpgSlmaRgqjqRZFiViABl0ASM6Kk-zNnOtDtCoYG9F0xjuHJqqUwpg4oNMZpc9_HzFEtbXBYN9rh34MilWUljVUpfw3lRJAyBKK_6BAayq4ONC3f9A7Pw4uHSYpAZwyyaqk3s3KDD6EAVu1G-xWD5NiVB2aV6l59bv5ZF_fJ47NFteP8qHqBM5nsLc9Tn9PUh-ubh4i83nDhog_Hzf08E2BLKRQt59Win-9vRTprGpV_AJ5ecV6</recordid><startdate>201602</startdate><enddate>201602</enddate><creator>Pereira, Brian</creator><creator>Zhang, Haoran</creator><creator>De Mey, Marjan</creator><creator>Lim, Chin Giaw</creator><creator>Li, Zheng-Jun</creator><creator>Stephanopoulos, Gregory</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><general>Wiley Blackwell (John Wiley & Sons)</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><scope>7QL</scope><scope>OTOTI</scope></search><sort><creationdate>201602</creationdate><title>Engineering a novel biosynthetic pathway in Escherichia coli for production of renewable ethylene glycol</title><author>Pereira, Brian ; Zhang, Haoran ; De Mey, Marjan ; Lim, Chin Giaw ; Li, Zheng-Jun ; Stephanopoulos, Gregory</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6927-abe4f7c65b6e4a9ccd6a83bf140bc6358066c474cb75ca0a534ee36a6a3667213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Antifreeze solutions</topic><topic>Biosynthesis</topic><topic>Biosynthetic Pathways - genetics</topic><topic>Biotechnology</topic><topic>Conversion</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - metabolism</topic><topic>Ethylene glycol</topic><topic>Ethylene Glycol - metabolism</topic><topic>Glucose</topic><topic>Glucose - metabolism</topic><topic>metabolic engineering</topic><topic>Metabolic Engineering - methods</topic><topic>Metabolism</topic><topic>Natural gas</topic><topic>Nonrenewable resources</topic><topic>Pathways</topic><topic>renewable</topic><topic>Renewable resources</topic><topic>serine</topic><topic>Serine - metabolism</topic><topic>Sugar</topic><topic>Sugars</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pereira, Brian</creatorcontrib><creatorcontrib>Zhang, Haoran</creatorcontrib><creatorcontrib>De Mey, Marjan</creatorcontrib><creatorcontrib>Lim, Chin Giaw</creatorcontrib><creatorcontrib>Li, Zheng-Jun</creatorcontrib><creatorcontrib>Stephanopoulos, Gregory</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>OSTI.GOV</collection><jtitle>Biotechnology and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pereira, Brian</au><au>Zhang, Haoran</au><au>De Mey, Marjan</au><au>Lim, Chin Giaw</au><au>Li, Zheng-Jun</au><au>Stephanopoulos, Gregory</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Engineering a novel biosynthetic pathway in Escherichia coli for production of renewable ethylene glycol</atitle><jtitle>Biotechnology and bioengineering</jtitle><addtitle>Biotechnol. 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Ethylene glycol (EG) is an important commodity chemical with broad industrial applications. It is presently produced from petroleum or natural gas feedstocks in processes requiring consumption of significant quantities of non‐renewable resources. Here, we report a novel pathway for biosynthesis of EG from the renewable sugar glucose in metabolically engineered Escherichia coli. Serine‐to‐EG conversion was first achieved through a pathway comprising serine decarboxylase, ethanolamine oxidase, and glycolaldehyde reductase. Serine provision in E. coli was then enhanced by overexpression of the serine‐biosynthesis pathway. The integration of these two parts into the complete EG‐biosynthesis pathway in E. coli allowed for production of 4.1 g/L EG at a cumulative yield of 0.14 g‐EG/g‐glucose, establishing a foundation for a promising biotechnology. Biotechnol. Bioeng. 2016;113: 376–383. © 2015 Wiley Periodicals, Inc.
A novel ethylene glycol (EG) biosynthetic pathway was engineered in bacterium in E. coli to achieve de novo production from renewable sugar glucose. The pathway includes the upstream part for serine biosynthesis and the downstream part for serine‐to‐EG conversion. Through metabolically engineering these two parts individually, we achieved production of 4.1 g/L EG at a yield of 0.14 g‐EG/g‐glucose.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>26221864</pmid><doi>10.1002/bit.25717</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Antifreeze solutions Biosynthesis Biosynthetic Pathways - genetics Biotechnology Conversion E coli Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Ethylene glycol Ethylene Glycol - metabolism Glucose Glucose - metabolism metabolic engineering Metabolic Engineering - methods Metabolism Natural gas Nonrenewable resources Pathways renewable Renewable resources serine Serine - metabolism Sugar Sugars |
| title | Engineering a novel biosynthetic pathway in Escherichia coli for production of renewable ethylene glycol |
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