An artificial metalloenzyme with the kinetics of native enzymes
Natural enzymes contain highly evolved active sites that lead to fast rates and high selectivities. Although artificial metalloenzymes have been developed that catalyze abiological transformations with high stereoselectivity, the activities of these artificial enzymes are much lower than those of na...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2016-10, Vol.354 (6308), p.102-106 |
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| container_title | Science (American Association for the Advancement of Science) |
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| creator | Dydio, P. Key, H. M. Nazarenko, A. Rha, J. Y.-E. Seyedkazemi, V. Clark, D. S. Hartwig, J. F. |
| description | Natural enzymes contain highly evolved active sites that lead to fast rates and high selectivities. Although artificial metalloenzymes have been developed that catalyze abiological transformations with high stereoselectivity, the activities of these artificial enzymes are much lower than those of natural enzymes. Here, we report a reconstituted artificial metalloenzyme containing an iridium porphyrin that exhibits kinetic parameters similar to those of natural enzymes. In particular, variants of the P450 enzyme CYP119 containing iridium in place of iron catalyze insertions of carbenes into C-H bonds with up to 98% enantiomeric 1 excess, 35,000 turnovers, and 2550 hours⁻¹ turnover frequency. This activity leads to intramolecular carbene insertions into unactivated C-H bonds and intermolecular carbene insertions into C-H bonds. These results lift the restrictions on merging chemical catalysis and biocatalysis to create highly active, productive, and selective metalloenzymes for abiological reactions. |
| doi_str_mv | 10.1126/science.aah4427 |
| format | Article |
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This activity leads to intramolecular carbene insertions into unactivated C-H bonds and intermolecular carbene insertions into C-H bonds. 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| ispartof | Science (American Association for the Advancement of Science), 2016-10, Vol.354 (6308), p.102-106 |
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| subjects | Biocatalysis Carbenes Catalysis Catalysts Chemical bonds Cytochrome P450 Family 19 - chemistry Cytochrome P450 Family 19 - genetics Enzymes Insertion Iridium Iridium - chemistry Kinetics Metallography Metalloproteins - chemistry Metalloproteins - genetics Methane - analogs & derivatives Methane - chemistry Mutation Porphyrins - chemistry Protein Conformation Reaction kinetics Stereoisomerism |
| title | An artificial metalloenzyme with the kinetics of native enzymes |
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