High-Resolution X-ray Photoelectron Spectroscopy of Mixed Silane Monolayers for DNA Attachment
The amine density of 3-aminopropyldimethylethoxysilane (APDMES) films on silica is controlled to determine its effect on DNA probe density and subsequent DNA hybridization. The amine density is tailored by controlling the surface reaction time of (1) APDMES, or (2) n-propyldimethylchlorosilane (PDMC...
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| Veröffentlicht in: | ACS applied materials & interfaces 2011-09, Vol.3 (9), p.3285-3292 |
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| creator | Shircliff, Rebecca A Martin, Ina T Pankow, Joel W Fennell, John Stradins, Paul Ghirardi, Maria L Cowley, Scott W Branz, Howard M |
| description | The amine density of 3-aminopropyldimethylethoxysilane (APDMES) films on silica is controlled to determine its effect on DNA probe density and subsequent DNA hybridization. The amine density is tailored by controlling the surface reaction time of (1) APDMES, or (2) n-propyldimethylchlorosilane (PDMCS, which is not amine terminated) and then reacting it with APDMES to form a mixed monolayer. High-resolution X-ray photoelectron spectroscopy (XPS) is used to quantify silane surface coverage of both pure and mixed monolayers on silica; the XPS data demonstrate control of amine density in both pure APDMES and PDMCS/APDMES mixed monolayers. A linear correlation between the atomic concentration of N atoms from the amine and Si atoms from the APDMES in pure APDMES films allows us to calculate the PDMCS/APDMES ratio in the mixed monolayers. Fluorescence from attached DNA probes and from hybridized DNA decreases as the percentage of APDMES in the mixed monolayer is decreased by dilution with PDMCS. |
| doi_str_mv | 10.1021/am200604q |
| format | Article |
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(NREL), Golden, CO (United States)</creatorcontrib><description>The amine density of 3-aminopropyldimethylethoxysilane (APDMES) films on silica is controlled to determine its effect on DNA probe density and subsequent DNA hybridization. The amine density is tailored by controlling the surface reaction time of (1) APDMES, or (2) n-propyldimethylchlorosilane (PDMCS, which is not amine terminated) and then reacting it with APDMES to form a mixed monolayer. High-resolution X-ray photoelectron spectroscopy (XPS) is used to quantify silane surface coverage of both pure and mixed monolayers on silica; the XPS data demonstrate control of amine density in both pure APDMES and PDMCS/APDMES mixed monolayers. A linear correlation between the atomic concentration of N atoms from the amine and Si atoms from the APDMES in pure APDMES films allows us to calculate the PDMCS/APDMES ratio in the mixed monolayers. Fluorescence from attached DNA probes and from hybridized DNA decreases as the percentage of APDMES in the mixed monolayer is decreased by dilution with PDMCS.</description><identifier>ISSN: 1944-8244</identifier><identifier>EISSN: 1944-8252</identifier><identifier>DOI: 10.1021/am200604q</identifier><identifier>PMID: 21797205</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Amines - chemistry ; DNA - chemistry ; DNA immobiilzation, DNA hybridization ; DNA Probes - chemistry ; Fluorescent Dyes - chemistry ; mixed monolayer ; Nucleic Acid Hybridization ; Photoelectron Spectroscopy ; Si 2p ; silanes ; Silanes - chemistry ; Silicon Dioxide - chemistry ; SOLAR ENERGY ; Surface Properties ; XPS</subject><ispartof>ACS applied materials & interfaces, 2011-09, Vol.3 (9), p.3285-3292</ispartof><rights>Copyright © 2011 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a407t-d9fbb138945c8ecf4353fe98f2f2d4e8715856cf8e1b9e59f8c317bee707fba03</citedby><cites>FETCH-LOGICAL-a407t-d9fbb138945c8ecf4353fe98f2f2d4e8715856cf8e1b9e59f8c317bee707fba03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/am200604q$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/am200604q$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,315,782,786,887,2769,27085,27933,27934,56747,56797</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21797205$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/1258567$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Shircliff, Rebecca A</creatorcontrib><creatorcontrib>Martin, Ina T</creatorcontrib><creatorcontrib>Pankow, Joel W</creatorcontrib><creatorcontrib>Fennell, John</creatorcontrib><creatorcontrib>Stradins, Paul</creatorcontrib><creatorcontrib>Ghirardi, Maria L</creatorcontrib><creatorcontrib>Cowley, Scott W</creatorcontrib><creatorcontrib>Branz, Howard M</creatorcontrib><creatorcontrib>National Renewable Energy Lab. (NREL), Golden, CO (United States)</creatorcontrib><title>High-Resolution X-ray Photoelectron Spectroscopy of Mixed Silane Monolayers for DNA Attachment</title><title>ACS applied materials & interfaces</title><addtitle>ACS Appl. Mater. Interfaces</addtitle><description>The amine density of 3-aminopropyldimethylethoxysilane (APDMES) films on silica is controlled to determine its effect on DNA probe density and subsequent DNA hybridization. The amine density is tailored by controlling the surface reaction time of (1) APDMES, or (2) n-propyldimethylchlorosilane (PDMCS, which is not amine terminated) and then reacting it with APDMES to form a mixed monolayer. High-resolution X-ray photoelectron spectroscopy (XPS) is used to quantify silane surface coverage of both pure and mixed monolayers on silica; the XPS data demonstrate control of amine density in both pure APDMES and PDMCS/APDMES mixed monolayers. A linear correlation between the atomic concentration of N atoms from the amine and Si atoms from the APDMES in pure APDMES films allows us to calculate the PDMCS/APDMES ratio in the mixed monolayers. Fluorescence from attached DNA probes and from hybridized DNA decreases as the percentage of APDMES in the mixed monolayer is decreased by dilution with PDMCS.</description><subject>Amines - chemistry</subject><subject>DNA - chemistry</subject><subject>DNA immobiilzation, DNA hybridization</subject><subject>DNA Probes - chemistry</subject><subject>Fluorescent Dyes - chemistry</subject><subject>mixed monolayer</subject><subject>Nucleic Acid Hybridization</subject><subject>Photoelectron Spectroscopy</subject><subject>Si 2p</subject><subject>silanes</subject><subject>Silanes - chemistry</subject><subject>Silicon Dioxide - chemistry</subject><subject>SOLAR ENERGY</subject><subject>Surface Properties</subject><subject>XPS</subject><issn>1944-8244</issn><issn>1944-8252</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0F1LwzAUBuAgis7phX9AgiDiRTVJ0zW9HPMTnIof4JUhzU5cpW22JAX3782c7sqrvIQnh5wXoQNKzihh9Fw1jJAB4fMN1KMF54lgGdtcZ8530K73n9GkjGTbaIfRvMhj7KH3m-pjmjyBt3UXKtvit8SpBX6c2mChBh1cvHue_QSv7WyBrcHj6gsm-LmqVQt4bFtbqwU4j411-OJ-iIchKD1toA17aMuo2sP-79lHr1eXL6Ob5O7h-nY0vEsUJ3lIJoUpS5qKgmdagDY8zVIDhTDMsAkHkdNMZANtBNCygKwwQqc0LwFykptSkbSPjlZzrQ-V9LoKoKfatm38uKRs-TqP6GSFZs7OO_BBNpXXUC_XsJ2XooiOswGL8nQlddzaOzBy5qpGuYWkRC4rl-vKoz38ndqVDUzW8q_jCI5XQGkvP23n2tjEP4O-AYFdiA0</recordid><startdate>20110928</startdate><enddate>20110928</enddate><creator>Shircliff, Rebecca A</creator><creator>Martin, Ina T</creator><creator>Pankow, Joel W</creator><creator>Fennell, John</creator><creator>Stradins, Paul</creator><creator>Ghirardi, Maria L</creator><creator>Cowley, Scott W</creator><creator>Branz, Howard M</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20110928</creationdate><title>High-Resolution X-ray Photoelectron Spectroscopy of Mixed Silane Monolayers for DNA Attachment</title><author>Shircliff, Rebecca A ; Martin, Ina T ; Pankow, Joel W ; Fennell, John ; Stradins, Paul ; Ghirardi, Maria L ; Cowley, Scott W ; Branz, Howard M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a407t-d9fbb138945c8ecf4353fe98f2f2d4e8715856cf8e1b9e59f8c317bee707fba03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Amines - chemistry</topic><topic>DNA - chemistry</topic><topic>DNA immobiilzation, DNA hybridization</topic><topic>DNA Probes - chemistry</topic><topic>Fluorescent Dyes - chemistry</topic><topic>mixed monolayer</topic><topic>Nucleic Acid Hybridization</topic><topic>Photoelectron Spectroscopy</topic><topic>Si 2p</topic><topic>silanes</topic><topic>Silanes - chemistry</topic><topic>Silicon Dioxide - chemistry</topic><topic>SOLAR ENERGY</topic><topic>Surface Properties</topic><topic>XPS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shircliff, Rebecca A</creatorcontrib><creatorcontrib>Martin, Ina T</creatorcontrib><creatorcontrib>Pankow, Joel W</creatorcontrib><creatorcontrib>Fennell, John</creatorcontrib><creatorcontrib>Stradins, Paul</creatorcontrib><creatorcontrib>Ghirardi, Maria L</creatorcontrib><creatorcontrib>Cowley, Scott W</creatorcontrib><creatorcontrib>Branz, Howard M</creatorcontrib><creatorcontrib>National Renewable Energy Lab. (NREL), Golden, CO (United States)</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>ACS applied materials & interfaces</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shircliff, Rebecca A</au><au>Martin, Ina T</au><au>Pankow, Joel W</au><au>Fennell, John</au><au>Stradins, Paul</au><au>Ghirardi, Maria L</au><au>Cowley, Scott W</au><au>Branz, Howard M</au><aucorp>National Renewable Energy Lab. (NREL), Golden, CO (United States)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-Resolution X-ray Photoelectron Spectroscopy of Mixed Silane Monolayers for DNA Attachment</atitle><jtitle>ACS applied materials & interfaces</jtitle><addtitle>ACS Appl. Mater. Interfaces</addtitle><date>2011-09-28</date><risdate>2011</risdate><volume>3</volume><issue>9</issue><spage>3285</spage><epage>3292</epage><pages>3285-3292</pages><issn>1944-8244</issn><eissn>1944-8252</eissn><abstract>The amine density of 3-aminopropyldimethylethoxysilane (APDMES) films on silica is controlled to determine its effect on DNA probe density and subsequent DNA hybridization. The amine density is tailored by controlling the surface reaction time of (1) APDMES, or (2) n-propyldimethylchlorosilane (PDMCS, which is not amine terminated) and then reacting it with APDMES to form a mixed monolayer. High-resolution X-ray photoelectron spectroscopy (XPS) is used to quantify silane surface coverage of both pure and mixed monolayers on silica; the XPS data demonstrate control of amine density in both pure APDMES and PDMCS/APDMES mixed monolayers. A linear correlation between the atomic concentration of N atoms from the amine and Si atoms from the APDMES in pure APDMES films allows us to calculate the PDMCS/APDMES ratio in the mixed monolayers. Fluorescence from attached DNA probes and from hybridized DNA decreases as the percentage of APDMES in the mixed monolayer is decreased by dilution with PDMCS.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>21797205</pmid><doi>10.1021/am200604q</doi><tpages>8</tpages></addata></record> |
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| subjects | Amines - chemistry DNA - chemistry DNA immobiilzation, DNA hybridization DNA Probes - chemistry Fluorescent Dyes - chemistry mixed monolayer Nucleic Acid Hybridization Photoelectron Spectroscopy Si 2p silanes Silanes - chemistry Silicon Dioxide - chemistry SOLAR ENERGY Surface Properties XPS |
| title | High-Resolution X-ray Photoelectron Spectroscopy of Mixed Silane Monolayers for DNA Attachment |
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