Surface and ultrastructural characterization of raw and pretreated switchgrass
The US Department of Energy-funded Biomass Refining CAFI (Consortium for Applied Fundamentals and Innovation) project has developed leading pretreatment technologies for application to switchgrass and has evaluated their effectiveness in recovering sugars from the coupled operations of pretreatment...
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| Veröffentlicht in: | Bioresource technology 2011-12, Vol.102 (24), p.11097-11104 |
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| creator | Donohoe, Bryon S. Vinzant, Todd B. Elander, Richard T. Pallapolu, Venkata Ramesh Lee, Y.Y. Garlock, Rebecca J. Balan, Venkatesh Dale, Bruce E. Kim, Youngmi Mosier, Nathan S. Ladisch, Michael R. Falls, Matthew Holtzapple, Mark T. Sierra-Ramirez, Rocio Shi, Jian Ebrik, Mirvat A. Redmond, Tim Yang, Bin Wyman, Charles E. Hames, Bonnie Thomas, Steve Warner, Ryan E. |
| description | The US Department of Energy-funded Biomass Refining CAFI (Consortium for Applied Fundamentals and Innovation) project has developed leading pretreatment technologies for application to switchgrass and has evaluated their effectiveness in recovering sugars from the coupled operations of pretreatment and enzymatic hydrolysis. Key chemical and physical characteristics have been determined for pretreated switchgrass samples. Several analytical microscopy approaches utilizing instruments in the Biomass Surface Characterization Laboratory (BSCL) at the National Renewable Energy Laboratory (NREL) have been applied to untreated and CAFI-pretreated switchgrass samples. The results of this work have shown that each of the CAFI pretreatment approaches on switchgrass result in different structural impacts at the plant tissue, cellular, and cell wall levels. Some of these structural changes can be related to changes in chemical composition upon pretreatment. There are also apparently different structural mechanisms that are responsible for achieving the highest enzymatic hydrolysis sugar yields. |
| doi_str_mv | 10.1016/j.biortech.2011.03.092 |
| format | Article |
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Key chemical and physical characteristics have been determined for pretreated switchgrass samples. Several analytical microscopy approaches utilizing instruments in the Biomass Surface Characterization Laboratory (BSCL) at the National Renewable Energy Laboratory (NREL) have been applied to untreated and CAFI-pretreated switchgrass samples. The results of this work have shown that each of the CAFI pretreatment approaches on switchgrass result in different structural impacts at the plant tissue, cellular, and cell wall levels. Some of these structural changes can be related to changes in chemical composition upon pretreatment. There are also apparently different structural mechanisms that are responsible for achieving the highest enzymatic hydrolysis sugar yields.</description><identifier>ISSN: 0960-8524</identifier><identifier>EISSN: 1873-2976</identifier><identifier>DOI: 10.1016/j.biortech.2011.03.092</identifier><identifier>PMID: 21571527</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>09 BIOMASS FUELS ; Agriculture ; Ammonia - metabolism ; BASIC BIOLOGICAL SCIENCES ; Biomass ; Biotechnology - methods ; Cell Wall - metabolism ; Cell Wall - ultrastructure ; Color ; Ecotype ; Enzymatic hydrolysis ; Hydrolysis ; Mathematical analysis ; Microfibrils - ultrastructure ; Microscopy ; Panicum - cytology ; Panicum - ultrastructure ; Porosity ; Pretreatment ; Refining ; Sugars ; Surface Properties ; Switchgrass ; Walls</subject><ispartof>Bioresource technology, 2011-12, Vol.102 (24), p.11097-11104</ispartof><rights>2011</rights><rights>Copyright © 2011. 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There are also apparently different structural mechanisms that are responsible for achieving the highest enzymatic hydrolysis sugar yields.</description><subject>09 BIOMASS FUELS</subject><subject>Agriculture</subject><subject>Ammonia - metabolism</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Biomass</subject><subject>Biotechnology - methods</subject><subject>Cell Wall - metabolism</subject><subject>Cell Wall - ultrastructure</subject><subject>Color</subject><subject>Ecotype</subject><subject>Enzymatic hydrolysis</subject><subject>Hydrolysis</subject><subject>Mathematical analysis</subject><subject>Microfibrils - ultrastructure</subject><subject>Microscopy</subject><subject>Panicum - cytology</subject><subject>Panicum - ultrastructure</subject><subject>Porosity</subject><subject>Pretreatment</subject><subject>Refining</subject><subject>Sugars</subject><subject>Surface Properties</subject><subject>Switchgrass</subject><subject>Walls</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi0EokvhL1QRF7gk9dhObN9AFV9SVQ7A2XLsCetVNllspxX8-jpsy5H25Dk874z8PoScAW2AQne-a_owx4xu2zAK0FDeUM2ekA0oyWumZfeUbKjuaK1aJk7Ii5R2lFIOkj0nJwxaCS2TG3L1bYmDdVjZyVfLmKNNOS4uL9GOldvaaF3GGP7YHOapmocq2pu_7CFijmgz-irdhOy2P0s0vSTPBjsmfHX3npIfHz98v_hcX3799OXi_WXthIZcCz8I3qP1HgeNaFUPnXPMC2_RdV4oNejyBW3bvve91GLglpehs55zyT0_Ja-Pe-eUg0kurE24eZrQZQOslRpUgd4coUOcfy2YstmH5HAc7YTzkowGgFZIIR8mqQTNmHrETiooCMV4Id_-lwQpJVCqAAraHVEX55QiDuYQw97G3waoWXWbnbnXbVbdhnJTdJfg2d2Npd-j_xe791uAd0cAi4zrgHHtCieHPsS1Kj-Hh27cAkKdwD8</recordid><startdate>20111201</startdate><enddate>20111201</enddate><creator>Donohoe, Bryon S.</creator><creator>Vinzant, Todd B.</creator><creator>Elander, Richard T.</creator><creator>Pallapolu, Venkata Ramesh</creator><creator>Lee, Y.Y.</creator><creator>Garlock, Rebecca J.</creator><creator>Balan, Venkatesh</creator><creator>Dale, Bruce E.</creator><creator>Kim, Youngmi</creator><creator>Mosier, Nathan S.</creator><creator>Ladisch, Michael R.</creator><creator>Falls, Matthew</creator><creator>Holtzapple, Mark T.</creator><creator>Sierra-Ramirez, Rocio</creator><creator>Shi, Jian</creator><creator>Ebrik, Mirvat A.</creator><creator>Redmond, Tim</creator><creator>Yang, Bin</creator><creator>Wyman, Charles E.</creator><creator>Hames, Bonnie</creator><creator>Thomas, Steve</creator><creator>Warner, Ryan E.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SU</scope><scope>7TB</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>KR7</scope><scope>7X8</scope><scope>7QO</scope><scope>7ST</scope><scope>7U6</scope><scope>P64</scope><scope>SOI</scope><scope>OTOTI</scope></search><sort><creationdate>20111201</creationdate><title>Surface and ultrastructural characterization of raw and pretreated switchgrass</title><author>Donohoe, Bryon S. ; Vinzant, Todd B. ; Elander, Richard T. ; Pallapolu, Venkata Ramesh ; Lee, Y.Y. ; Garlock, Rebecca J. ; Balan, Venkatesh ; Dale, Bruce E. ; Kim, Youngmi ; Mosier, Nathan S. ; Ladisch, Michael R. ; Falls, Matthew ; Holtzapple, Mark T. ; Sierra-Ramirez, Rocio ; Shi, Jian ; Ebrik, Mirvat A. ; Redmond, Tim ; Yang, Bin ; Wyman, Charles E. ; Hames, Bonnie ; Thomas, Steve ; Warner, Ryan E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c491t-4df43beaddef9eea8b16cc2d4daec6d488f99769a5bbdb794f3a3db76ad3373d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>09 BIOMASS FUELS</topic><topic>Agriculture</topic><topic>Ammonia - 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| fulltext | fulltext |
| identifier | ISSN: 0960-8524 |
| ispartof | Bioresource technology, 2011-12, Vol.102 (24), p.11097-11104 |
| issn | 0960-8524 1873-2976 |
| language | eng |
| recordid | cdi_osti_scitechconnect_1257918 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 09 BIOMASS FUELS Agriculture Ammonia - metabolism BASIC BIOLOGICAL SCIENCES Biomass Biotechnology - methods Cell Wall - metabolism Cell Wall - ultrastructure Color Ecotype Enzymatic hydrolysis Hydrolysis Mathematical analysis Microfibrils - ultrastructure Microscopy Panicum - cytology Panicum - ultrastructure Porosity Pretreatment Refining Sugars Surface Properties Switchgrass Walls |
| title | Surface and ultrastructural characterization of raw and pretreated switchgrass |
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