Adsorption Kinetics Dictate Monolayer Self-Assembly for Both Lipid-In and Lipid-Out Approaches to Droplet Interface Bilayer Formation
The droplet interface bilayer (DIB)a method to assemble planar lipid bilayer membranes between lipid-coated aqueous dropletshas gained popularity among researchers in many fields. Well-packed lipid monolayer on aqueous droplet–oil interfaces is a prerequisite for successfully assembling DIBs. Such...
Gespeichert in:
Veröffentlicht in: | Langmuir 2015-12, Vol.31 (47), p.12883-12893 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 12893 |
---|---|
container_issue | 47 |
container_start_page | 12883 |
container_title | Langmuir |
container_volume | 31 |
creator | Venkatesan, Guru A Lee, Joonho Farimani, Amir Barati Heiranian, Mohammad Collier, C. Patrick Aluru, Narayana R Sarles, Stephen A |
description | The droplet interface bilayer (DIB)a method to assemble planar lipid bilayer membranes between lipid-coated aqueous dropletshas gained popularity among researchers in many fields. Well-packed lipid monolayer on aqueous droplet–oil interfaces is a prerequisite for successfully assembling DIBs. Such monolayers can be achieved by two different techniques: “lipid-in”, in which phospholipids in the form of liposomes are placed in water, and “lipid-out”, in which phospholipids are placed in oil as inverse micelles. While both approaches are capable of monolayer assembly needed for bilayer formation, droplet pairs assembled with these two techniques require significantly different incubation periods and exhibit different success rates for bilayer formation. In this study, we combine experimental interfacial tension measurements with molecular dynamics simulations of phospholipids (DPhPC and DOPC) assembled from water and oil origins to understand the differences in kinetics of monolayer formation. With the results from simulations and by using a simplified model to analyze dynamic interfacial tensions, we conclude that, at high lipid concentrations common to DIBs, monolayer formation is simple adsorption controlled for lipid-in technique, whereas it is predominantly adsorption-barrier controlled for the lipid-out technique due to the interaction of interface-bound lipids with lipid structures in the subsurface. The adsorption barrier established in lipid-out technique leads to a prolonged incubation time and lower bilayer formation success rate, proving a good correlation between interfacial tension measurements and bilayer formation. We also clarify that advective flow expedites monolayer formation and improves bilayer formation success rate by disrupting lipid structures, rather than enhancing diffusion, in the subsurface and at the interface for lipid-out technique. Additionally, electrical properties of DIBs formed with varying lipid placement and type are characterized. |
doi_str_mv | 10.1021/acs.langmuir.5b02293 |
format | Article |
fullrecord | <record><control><sourceid>proquest_osti_</sourceid><recordid>TN_cdi_osti_scitechconnect_1237604</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1738813819</sourcerecordid><originalsourceid>FETCH-LOGICAL-a356t-a40e83f3524de1c10a4d1408ef56a4409424e3e6688b466b1cfee85452f39fa63</originalsourceid><addsrcrecordid>eNp9kc1u1DAURi0EokPhDRCyWLHJ4P8ky2lLYcSgLoC15TjXjKvEDrazmAfgvckoU5asrCud77vyPQi9pWRLCaMfjc3bwYRf4-zTVnaEsZY_QxsqGalkw-rnaENqwataKH6FXuX8SAhpuWhfoiumpFSM1Rv0Z9fnmKbiY8BffYDibcZ33hZTAH-LIQ7mBAl_h8FVu5xh7IYTdjHhm1iO-OAn31f7gE3oL8PDXPBumlI09ggZl4jvUpwGKHgfCiRnLOAbv7bexzSa8-rX6IUzQ4Y3l_ca_bz_9OP2S3V4-Ly_3R0qw6UqlREEGu64ZKIHaikxoqeCNOCkMkKQVjABHJRqmk4o1VHrABopJHO8dUbxa_R-7Y25eJ2tL2CPNoYAtmjKeK2IWKAPK7R84vcMuejRZwvDcmyIc9a05k1DeUPbBRUralPMOYHTU_KjSSdNiT5b0osl_WRJXywtsXeXDXM3Qv8v9KRlAcgKnOOPcU5hucr_O_8C7UKiVA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1738813819</pqid></control><display><type>article</type><title>Adsorption Kinetics Dictate Monolayer Self-Assembly for Both Lipid-In and Lipid-Out Approaches to Droplet Interface Bilayer Formation</title><source>MEDLINE</source><source>ACS Publications</source><creator>Venkatesan, Guru A ; Lee, Joonho ; Farimani, Amir Barati ; Heiranian, Mohammad ; Collier, C. Patrick ; Aluru, Narayana R ; Sarles, Stephen A</creator><creatorcontrib>Venkatesan, Guru A ; Lee, Joonho ; Farimani, Amir Barati ; Heiranian, Mohammad ; Collier, C. Patrick ; Aluru, Narayana R ; Sarles, Stephen A ; Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)</creatorcontrib><description>The droplet interface bilayer (DIB)a method to assemble planar lipid bilayer membranes between lipid-coated aqueous dropletshas gained popularity among researchers in many fields. Well-packed lipid monolayer on aqueous droplet–oil interfaces is a prerequisite for successfully assembling DIBs. Such monolayers can be achieved by two different techniques: “lipid-in”, in which phospholipids in the form of liposomes are placed in water, and “lipid-out”, in which phospholipids are placed in oil as inverse micelles. While both approaches are capable of monolayer assembly needed for bilayer formation, droplet pairs assembled with these two techniques require significantly different incubation periods and exhibit different success rates for bilayer formation. In this study, we combine experimental interfacial tension measurements with molecular dynamics simulations of phospholipids (DPhPC and DOPC) assembled from water and oil origins to understand the differences in kinetics of monolayer formation. With the results from simulations and by using a simplified model to analyze dynamic interfacial tensions, we conclude that, at high lipid concentrations common to DIBs, monolayer formation is simple adsorption controlled for lipid-in technique, whereas it is predominantly adsorption-barrier controlled for the lipid-out technique due to the interaction of interface-bound lipids with lipid structures in the subsurface. The adsorption barrier established in lipid-out technique leads to a prolonged incubation time and lower bilayer formation success rate, proving a good correlation between interfacial tension measurements and bilayer formation. We also clarify that advective flow expedites monolayer formation and improves bilayer formation success rate by disrupting lipid structures, rather than enhancing diffusion, in the subsurface and at the interface for lipid-out technique. Additionally, electrical properties of DIBs formed with varying lipid placement and type are characterized.</description><identifier>ISSN: 0743-7463</identifier><identifier>EISSN: 1520-5827</identifier><identifier>DOI: 10.1021/acs.langmuir.5b02293</identifier><identifier>PMID: 26556227</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Adsorption ; INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY ; Kinetics ; Lipid Bilayers - chemistry ; Phospholipids - chemistry</subject><ispartof>Langmuir, 2015-12, Vol.31 (47), p.12883-12893</ispartof><rights>Copyright © 2015 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a356t-a40e83f3524de1c10a4d1408ef56a4409424e3e6688b466b1cfee85452f39fa63</citedby><cites>FETCH-LOGICAL-a356t-a40e83f3524de1c10a4d1408ef56a4409424e3e6688b466b1cfee85452f39fa63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.langmuir.5b02293$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.langmuir.5b02293$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,314,780,784,885,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26556227$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/1237604$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Venkatesan, Guru A</creatorcontrib><creatorcontrib>Lee, Joonho</creatorcontrib><creatorcontrib>Farimani, Amir Barati</creatorcontrib><creatorcontrib>Heiranian, Mohammad</creatorcontrib><creatorcontrib>Collier, C. Patrick</creatorcontrib><creatorcontrib>Aluru, Narayana R</creatorcontrib><creatorcontrib>Sarles, Stephen A</creatorcontrib><creatorcontrib>Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)</creatorcontrib><title>Adsorption Kinetics Dictate Monolayer Self-Assembly for Both Lipid-In and Lipid-Out Approaches to Droplet Interface Bilayer Formation</title><title>Langmuir</title><addtitle>Langmuir</addtitle><description>The droplet interface bilayer (DIB)a method to assemble planar lipid bilayer membranes between lipid-coated aqueous dropletshas gained popularity among researchers in many fields. Well-packed lipid monolayer on aqueous droplet–oil interfaces is a prerequisite for successfully assembling DIBs. Such monolayers can be achieved by two different techniques: “lipid-in”, in which phospholipids in the form of liposomes are placed in water, and “lipid-out”, in which phospholipids are placed in oil as inverse micelles. While both approaches are capable of monolayer assembly needed for bilayer formation, droplet pairs assembled with these two techniques require significantly different incubation periods and exhibit different success rates for bilayer formation. In this study, we combine experimental interfacial tension measurements with molecular dynamics simulations of phospholipids (DPhPC and DOPC) assembled from water and oil origins to understand the differences in kinetics of monolayer formation. With the results from simulations and by using a simplified model to analyze dynamic interfacial tensions, we conclude that, at high lipid concentrations common to DIBs, monolayer formation is simple adsorption controlled for lipid-in technique, whereas it is predominantly adsorption-barrier controlled for the lipid-out technique due to the interaction of interface-bound lipids with lipid structures in the subsurface. The adsorption barrier established in lipid-out technique leads to a prolonged incubation time and lower bilayer formation success rate, proving a good correlation between interfacial tension measurements and bilayer formation. We also clarify that advective flow expedites monolayer formation and improves bilayer formation success rate by disrupting lipid structures, rather than enhancing diffusion, in the subsurface and at the interface for lipid-out technique. Additionally, electrical properties of DIBs formed with varying lipid placement and type are characterized.</description><subject>Adsorption</subject><subject>INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY</subject><subject>Kinetics</subject><subject>Lipid Bilayers - chemistry</subject><subject>Phospholipids - chemistry</subject><issn>0743-7463</issn><issn>1520-5827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u1DAURi0EokPhDRCyWLHJ4P8ky2lLYcSgLoC15TjXjKvEDrazmAfgvckoU5asrCud77vyPQi9pWRLCaMfjc3bwYRf4-zTVnaEsZY_QxsqGalkw-rnaENqwataKH6FXuX8SAhpuWhfoiumpFSM1Rv0Z9fnmKbiY8BffYDibcZ33hZTAH-LIQ7mBAl_h8FVu5xh7IYTdjHhm1iO-OAn31f7gE3oL8PDXPBumlI09ggZl4jvUpwGKHgfCiRnLOAbv7bexzSa8-rX6IUzQ4Y3l_ca_bz_9OP2S3V4-Ly_3R0qw6UqlREEGu64ZKIHaikxoqeCNOCkMkKQVjABHJRqmk4o1VHrABopJHO8dUbxa_R-7Y25eJ2tL2CPNoYAtmjKeK2IWKAPK7R84vcMuejRZwvDcmyIc9a05k1DeUPbBRUralPMOYHTU_KjSSdNiT5b0osl_WRJXywtsXeXDXM3Qv8v9KRlAcgKnOOPcU5hucr_O_8C7UKiVA</recordid><startdate>20151201</startdate><enddate>20151201</enddate><creator>Venkatesan, Guru A</creator><creator>Lee, Joonho</creator><creator>Farimani, Amir Barati</creator><creator>Heiranian, Mohammad</creator><creator>Collier, C. Patrick</creator><creator>Aluru, Narayana R</creator><creator>Sarles, Stephen A</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20151201</creationdate><title>Adsorption Kinetics Dictate Monolayer Self-Assembly for Both Lipid-In and Lipid-Out Approaches to Droplet Interface Bilayer Formation</title><author>Venkatesan, Guru A ; Lee, Joonho ; Farimani, Amir Barati ; Heiranian, Mohammad ; Collier, C. Patrick ; Aluru, Narayana R ; Sarles, Stephen A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a356t-a40e83f3524de1c10a4d1408ef56a4409424e3e6688b466b1cfee85452f39fa63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adsorption</topic><topic>INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY</topic><topic>Kinetics</topic><topic>Lipid Bilayers - chemistry</topic><topic>Phospholipids - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Venkatesan, Guru A</creatorcontrib><creatorcontrib>Lee, Joonho</creatorcontrib><creatorcontrib>Farimani, Amir Barati</creatorcontrib><creatorcontrib>Heiranian, Mohammad</creatorcontrib><creatorcontrib>Collier, C. Patrick</creatorcontrib><creatorcontrib>Aluru, Narayana R</creatorcontrib><creatorcontrib>Sarles, Stephen A</creatorcontrib><creatorcontrib>Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Langmuir</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Venkatesan, Guru A</au><au>Lee, Joonho</au><au>Farimani, Amir Barati</au><au>Heiranian, Mohammad</au><au>Collier, C. Patrick</au><au>Aluru, Narayana R</au><au>Sarles, Stephen A</au><aucorp>Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adsorption Kinetics Dictate Monolayer Self-Assembly for Both Lipid-In and Lipid-Out Approaches to Droplet Interface Bilayer Formation</atitle><jtitle>Langmuir</jtitle><addtitle>Langmuir</addtitle><date>2015-12-01</date><risdate>2015</risdate><volume>31</volume><issue>47</issue><spage>12883</spage><epage>12893</epage><pages>12883-12893</pages><issn>0743-7463</issn><eissn>1520-5827</eissn><abstract>The droplet interface bilayer (DIB)a method to assemble planar lipid bilayer membranes between lipid-coated aqueous dropletshas gained popularity among researchers in many fields. Well-packed lipid monolayer on aqueous droplet–oil interfaces is a prerequisite for successfully assembling DIBs. Such monolayers can be achieved by two different techniques: “lipid-in”, in which phospholipids in the form of liposomes are placed in water, and “lipid-out”, in which phospholipids are placed in oil as inverse micelles. While both approaches are capable of monolayer assembly needed for bilayer formation, droplet pairs assembled with these two techniques require significantly different incubation periods and exhibit different success rates for bilayer formation. In this study, we combine experimental interfacial tension measurements with molecular dynamics simulations of phospholipids (DPhPC and DOPC) assembled from water and oil origins to understand the differences in kinetics of monolayer formation. With the results from simulations and by using a simplified model to analyze dynamic interfacial tensions, we conclude that, at high lipid concentrations common to DIBs, monolayer formation is simple adsorption controlled for lipid-in technique, whereas it is predominantly adsorption-barrier controlled for the lipid-out technique due to the interaction of interface-bound lipids with lipid structures in the subsurface. The adsorption barrier established in lipid-out technique leads to a prolonged incubation time and lower bilayer formation success rate, proving a good correlation between interfacial tension measurements and bilayer formation. We also clarify that advective flow expedites monolayer formation and improves bilayer formation success rate by disrupting lipid structures, rather than enhancing diffusion, in the subsurface and at the interface for lipid-out technique. Additionally, electrical properties of DIBs formed with varying lipid placement and type are characterized.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>26556227</pmid><doi>10.1021/acs.langmuir.5b02293</doi><tpages>11</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0743-7463 |
ispartof | Langmuir, 2015-12, Vol.31 (47), p.12883-12893 |
issn | 0743-7463 1520-5827 |
language | eng |
recordid | cdi_osti_scitechconnect_1237604 |
source | MEDLINE; ACS Publications |
subjects | Adsorption INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY Kinetics Lipid Bilayers - chemistry Phospholipids - chemistry |
title | Adsorption Kinetics Dictate Monolayer Self-Assembly for Both Lipid-In and Lipid-Out Approaches to Droplet Interface Bilayer Formation |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T17%3A02%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_osti_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Adsorption%20Kinetics%20Dictate%20Monolayer%20Self-Assembly%20for%20Both%20Lipid-In%20and%20Lipid-Out%20Approaches%20to%20Droplet%20Interface%20Bilayer%20Formation&rft.jtitle=Langmuir&rft.au=Venkatesan,%20Guru%20A&rft.aucorp=Oak%20Ridge%20National%20Laboratory%20(ORNL),%20Oak%20Ridge,%20TN%20(United%20States).%20Center%20for%20Nanophase%20Materials%20Sciences%20(CNMS)&rft.date=2015-12-01&rft.volume=31&rft.issue=47&rft.spage=12883&rft.epage=12893&rft.pages=12883-12893&rft.issn=0743-7463&rft.eissn=1520-5827&rft_id=info:doi/10.1021/acs.langmuir.5b02293&rft_dat=%3Cproquest_osti_%3E1738813819%3C/proquest_osti_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1738813819&rft_id=info:pmid/26556227&rfr_iscdi=true |