Effect of Divalent Metals on Hg(II) Uptake and Methylation by Bacteria
The production of methylmercury by some bacteria is a key first step in the accumulation and biomagnification of this toxic substance in aquatic food webs, a major human health concern. By direct measurement of cellular Hg(II) uptake in model iron and sulfate reducing bacteria, we have observed tha...
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| Veröffentlicht in: | Environmental science & technology 2014-03, Vol.48 (5), p.3007-3013 |
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| creator | Schaefer, Jeffra K Szczuka, Aleksandra Morel, François M. M |
| description | The production of methylmercury by some bacteria is a key first step in the accumulation and biomagnification of this toxic substance in aquatic food webs, a major human health concern. By direct measurement of cellular Hg(II) uptake in model iron and sulfate reducing bacteria, we have observed that specific trace metals, such as Zn(II) and Cd(II), inhibit uptake and methylation in these organisms, whereas other metals, such as Ni(II), Co(II), or Fe(II), do not. The inhibition of Hg(II) methylation by Zn(II) was competitive in nature and related to the concentration of inorganically complexed Zn(II) (Zn′). The inhibition of Hg(II) methylation was alleviated by decreasing the free Zn′ concentration through complexation with nitrilotriacetic acid without altering the speciation of Hg(II). The inhibitory effect by Zn(II) was observed when either Hg-cysteine complexes or neutral HgCl2 dominated the speciation of Hg(II), demonstrating that both charged and neutral species are transported into the cytosol by an active rather than passive process. We propose that Hg(II) uptake is the result of its accidental uptake by metal transporter(s), possibly one effecting the transport of Zn(II). |
| doi_str_mv | 10.1021/es405215v |
| format | Article |
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M</creator><creatorcontrib>Schaefer, Jeffra K ; Szczuka, Aleksandra ; Morel, François M. M</creatorcontrib><description>The production of methylmercury by some bacteria is a key first step in the accumulation and biomagnification of this toxic substance in aquatic food webs, a major human health concern. By direct measurement of cellular Hg(II) uptake in model iron and sulfate reducing bacteria, we have observed that specific trace metals, such as Zn(II) and Cd(II), inhibit uptake and methylation in these organisms, whereas other metals, such as Ni(II), Co(II), or Fe(II), do not. The inhibition of Hg(II) methylation by Zn(II) was competitive in nature and related to the concentration of inorganically complexed Zn(II) (Zn′). The inhibition of Hg(II) methylation was alleviated by decreasing the free Zn′ concentration through complexation with nitrilotriacetic acid without altering the speciation of Hg(II). The inhibitory effect by Zn(II) was observed when either Hg-cysteine complexes or neutral HgCl2 dominated the speciation of Hg(II), demonstrating that both charged and neutral species are transported into the cytosol by an active rather than passive process. We propose that Hg(II) uptake is the result of its accidental uptake by metal transporter(s), possibly one effecting the transport of Zn(II).</description><identifier>ISSN: 0013-936X</identifier><identifier>EISSN: 1520-5851</identifier><identifier>DOI: 10.1021/es405215v</identifier><identifier>PMID: 24512453</identifier><identifier>CODEN: ESTHAG</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Animal, plant and microbial ecology ; Applied ecology ; Bacteria ; Bioaccumulation ; Biological and medical sciences ; Desulfovibrio desulfuricans - drug effects ; Desulfovibrio desulfuricans - metabolism ; DNA methylation ; Ecotoxicology, biological effects of pollution ; Effects ; Fresh water environment ; Fundamental and applied biological sciences. Psychology ; Geobacter - drug effects ; Geobacter - metabolism ; Mercury - metabolism ; Metals ; Metals, Heavy - pharmacology ; Methylmercury Compounds - metabolism ; Nitrilotriacetic Acid - metabolism ; Terrestrial environment, soil, air</subject><ispartof>Environmental science & technology, 2014-03, Vol.48 (5), p.3007-3013</ispartof><rights>Copyright © 2014 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><rights>Copyright American Chemical Society Mar 4, 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a468t-b1e3c1f59911dcc230aae31ff5629e5426b61430bcf5e4764e16d9500a7234963</citedby><cites>FETCH-LOGICAL-a468t-b1e3c1f59911dcc230aae31ff5629e5426b61430bcf5e4764e16d9500a7234963</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/es405215v$$EPDF$$P50$$Gacs$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/es405215v$$EHTML$$P50$$Gacs$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28318588$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24512453$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/1179592$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Schaefer, Jeffra K</creatorcontrib><creatorcontrib>Szczuka, Aleksandra</creatorcontrib><creatorcontrib>Morel, François M. M</creatorcontrib><title>Effect of Divalent Metals on Hg(II) Uptake and Methylation by Bacteria</title><title>Environmental science & technology</title><addtitle>Environ. Sci. Technol</addtitle><description>The production of methylmercury by some bacteria is a key first step in the accumulation and biomagnification of this toxic substance in aquatic food webs, a major human health concern. By direct measurement of cellular Hg(II) uptake in model iron and sulfate reducing bacteria, we have observed that specific trace metals, such as Zn(II) and Cd(II), inhibit uptake and methylation in these organisms, whereas other metals, such as Ni(II), Co(II), or Fe(II), do not. The inhibition of Hg(II) methylation by Zn(II) was competitive in nature and related to the concentration of inorganically complexed Zn(II) (Zn′). The inhibition of Hg(II) methylation was alleviated by decreasing the free Zn′ concentration through complexation with nitrilotriacetic acid without altering the speciation of Hg(II). The inhibitory effect by Zn(II) was observed when either Hg-cysteine complexes or neutral HgCl2 dominated the speciation of Hg(II), demonstrating that both charged and neutral species are transported into the cytosol by an active rather than passive process. We propose that Hg(II) uptake is the result of its accidental uptake by metal transporter(s), possibly one effecting the transport of Zn(II).</description><subject>Animal, plant and microbial ecology</subject><subject>Applied ecology</subject><subject>Bacteria</subject><subject>Bioaccumulation</subject><subject>Biological and medical sciences</subject><subject>Desulfovibrio desulfuricans - drug effects</subject><subject>Desulfovibrio desulfuricans - metabolism</subject><subject>DNA methylation</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>Effects</subject><subject>Fresh water environment</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Geobacter - drug effects</subject><subject>Geobacter - metabolism</subject><subject>Mercury - metabolism</subject><subject>Metals</subject><subject>Metals, Heavy - pharmacology</subject><subject>Methylmercury Compounds - metabolism</subject><subject>Nitrilotriacetic Acid - metabolism</subject><subject>Terrestrial environment, soil, air</subject><issn>0013-936X</issn><issn>1520-5851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>N~.</sourceid><sourceid>EIF</sourceid><recordid>eNpl0UtLAzEQAOAgitbHwT8giyLoYTWTbNLN0bcFxYuCt2U2nejqdrdu0kL_vSmtD_QQcpgv88gwtgv8BLiAU_IZVwLUdIX1QAmeqlzBKutxDjI1Uj9vsE3v3zjnQvJ8nW2ITEE8sseur5wjG5LWJZfVFGtqQnJPAWuftE1y-3I0GBwnT-OA75RgM5zHXmc1hipGy1lyjjZQV-E2W3PxDe0s7y32dH31eHGb3j3cDC7O7lLMdB7SEkhacMoYgKG1sRtEkuCc0sKQyoQuNWSSl9Ypyvo6I9BDozjHvpCZ0XKL7S_ytj5UhbdVIPtq26aJMxQAfaOMiOhogcZd-zEhH4pR5S3VNTbUTnwBcXgppNQ80oM_9K2ddE0cISqujYl9zaseL5TtWu87csW4q0bYzQrgxXwDxfcGot1bZpyUIxp-y68vj-BwCdBbrF2Hja38j8sl5CrPfxxa_6urfwU_AYQ6lSs</recordid><startdate>20140304</startdate><enddate>20140304</enddate><creator>Schaefer, Jeffra K</creator><creator>Szczuka, Aleksandra</creator><creator>Morel, François M. 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Psychology</topic><topic>Geobacter - drug effects</topic><topic>Geobacter - metabolism</topic><topic>Mercury - metabolism</topic><topic>Metals</topic><topic>Metals, Heavy - pharmacology</topic><topic>Methylmercury Compounds - metabolism</topic><topic>Nitrilotriacetic Acid - metabolism</topic><topic>Terrestrial environment, soil, air</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schaefer, Jeffra K</creatorcontrib><creatorcontrib>Szczuka, Aleksandra</creatorcontrib><creatorcontrib>Morel, François M. 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| subjects | Animal, plant and microbial ecology Applied ecology Bacteria Bioaccumulation Biological and medical sciences Desulfovibrio desulfuricans - drug effects Desulfovibrio desulfuricans - metabolism DNA methylation Ecotoxicology, biological effects of pollution Effects Fresh water environment Fundamental and applied biological sciences. Psychology Geobacter - drug effects Geobacter - metabolism Mercury - metabolism Metals Metals, Heavy - pharmacology Methylmercury Compounds - metabolism Nitrilotriacetic Acid - metabolism Terrestrial environment, soil, air |
| title | Effect of Divalent Metals on Hg(II) Uptake and Methylation by Bacteria |
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