Environmental Survey for Four Pathogenic Bacteria and Closely Related Species Using Phylogenetic and Functional Genes
Bacterial species with high DNA sequence similarity to pathogens could affect the specificity of assays designed to detect biological threat agents in environmental samples. The natural presence of four pathogenic bacteria, Bacillus anthracis, Clostridium perfringens, Francisella tularensis, and Yer...
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| Veröffentlicht in: | Journal of forensic sciences 2006-05, Vol.51 (3), p.548-558 |
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| creator | Kuske, Cheryl R. Barns, Susan M. Grow, Christy C. Merrill, Lori Dunbar, John |
| description | Bacterial species with high DNA sequence similarity to pathogens could affect the specificity of assays designed to detect biological threat agents in environmental samples. The natural presence of four pathogenic bacteria, Bacillus anthracis, Clostridium perfringens, Francisella tularensis, and Yersinia pestis and their closely related species, was determined for a large collection of soil and aerosol samples. Polymerase chain reaction (PCR) and gene sequencing were used using group‐specific 16S rRNA primers to identify pathogens and related species, and pathogen‐specific virulence genes. Close relatives of B. anthracis (B. cereus group species) were detected in 37% of the soils and 25% of the aerosol samples. The B. anthracis protective antigen (pag) gene or a close homolog was detected in 16 of these samples. For the other three pathogen groups, the frequency of detection was much lower, and none of the samples were positive with both the phylogenetic and virulence gene primer sets. |
| doi_str_mv | 10.1111/j.1556-4029.2006.00131.x |
| format | Article |
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The natural presence of four pathogenic bacteria, Bacillus anthracis, Clostridium perfringens, Francisella tularensis, and Yersinia pestis and their closely related species, was determined for a large collection of soil and aerosol samples. Polymerase chain reaction (PCR) and gene sequencing were used using group‐specific 16S rRNA primers to identify pathogens and related species, and pathogen‐specific virulence genes. Close relatives of B. anthracis (B. cereus group species) were detected in 37% of the soils and 25% of the aerosol samples. The B. anthracis protective antigen (pag) gene or a close homolog was detected in 16 of these samples. 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The natural presence of four pathogenic bacteria, Bacillus anthracis, Clostridium perfringens, Francisella tularensis, and Yersinia pestis and their closely related species, was determined for a large collection of soil and aerosol samples. Polymerase chain reaction (PCR) and gene sequencing were used using group‐specific 16S rRNA primers to identify pathogens and related species, and pathogen‐specific virulence genes. Close relatives of B. anthracis (B. cereus group species) were detected in 37% of the soils and 25% of the aerosol samples. The B. anthracis protective antigen (pag) gene or a close homolog was detected in 16 of these samples. For the other three pathogen groups, the frequency of detection was much lower, and none of the samples were positive with both the phylogenetic and virulence gene primer sets.</description><subject>aerosol</subject><subject>Aerosols</subject><subject>Bacillus anthracis</subject><subject>Bacillus anthracis - genetics</subject><subject>Bacillus anthracis - isolation & purification</subject><subject>backgrounds</subject><subject>Bacteria</subject><subject>Clostridium perfringens</subject><subject>Clostridium perfringens - genetics</subject><subject>Clostridium perfringens - isolation & purification</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Primers</subject><subject>DNA, Bacterial - analysis</subject><subject>DNA, Ribosomal - analysis</subject><subject>environmental detection of biothreat agents</subject><subject>Environmental Monitoring</subject><subject>Enzymes</subject><subject>Forensic Medicine</subject><subject>forensic science</subject><subject>Francisella tularensis</subject><subject>Francisella tularensis - genetics</subject><subject>Francisella tularensis - isolation & purification</subject><subject>Genes</subject><subject>Pathogens</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction</subject><subject>select agent</subject><subject>Sequence Analysis, DNA</subject><subject>soil</subject><subject>Soil Microbiology</subject><subject>Yersinia pestis</subject><subject>Yersinia pestis - genetics</subject><subject>Yersinia pestis - isolation & purification</subject><issn>0022-1198</issn><issn>1556-4029</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1uEzEUhS0EoqHwCshiP4M9Px5bYkOjJlC1tBAKEhvLce40DhM72J6Seft6SNRu8caW_Z3je-9BCFOS07Teb3Ja1yyrSCHyghCWE0JLmu-focnjw3M0IaQoMkoFP0GvQtiQRFJGX6ITyphgDaET1J_be-Od3YKNqsOL3t_DgFvn8cz1Ht-ouHZ3YI3GZ0pH8EZhZVd42rkA3YC_QacirPBiB9pAwLfB2Dt8sx66UQUx6UZ81lsdjbPph3m6Dq_Ri1Z1Ad4c91N0Ozv_Pv2UXV7PP08_Xma6qjnNRCOaGsRSF7qGstRaVYSvNOOpjWXRsqZuWyiVrkTVipa3SiloWN0UrAQBXJWn6N3B14VoZNAmgl5rZy3oKCmtS17wJ2jn3Z8eQpSb1HqqNciCCsZ5qiVB_ABp70Lw0MqdN1vlB0mJHDORGzmOXo6jl2Mm8l8mcp-kb4_-_XILqyfhMYQEfDgAf00Hw38by4vZdTokeXaQmxBh_yhX_rdkTdnU8ueXubyiF2Tx9eyH_FU-APeAqkE</recordid><startdate>200605</startdate><enddate>200605</enddate><creator>Kuske, Cheryl R.</creator><creator>Barns, Susan M.</creator><creator>Grow, Christy C.</creator><creator>Merrill, Lori</creator><creator>Dunbar, John</creator><general>Blackwell Publishing Inc</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K7.</scope><scope>OTOTI</scope></search><sort><creationdate>200605</creationdate><title>Environmental Survey for Four Pathogenic Bacteria and Closely Related Species Using Phylogenetic and Functional Genes</title><author>Kuske, Cheryl R. ; Barns, Susan M. ; Grow, Christy C. ; Merrill, Lori ; Dunbar, John</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4581-97975e9bc2c5e33cca408dc68616b2f675ffe3ac494f9f8faaae7657263e9e8a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>aerosol</topic><topic>Aerosols</topic><topic>Bacillus anthracis</topic><topic>Bacillus anthracis - genetics</topic><topic>Bacillus anthracis - isolation & purification</topic><topic>backgrounds</topic><topic>Bacteria</topic><topic>Clostridium perfringens</topic><topic>Clostridium perfringens - genetics</topic><topic>Clostridium perfringens - isolation & purification</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA Primers</topic><topic>DNA, Bacterial - analysis</topic><topic>DNA, Ribosomal - analysis</topic><topic>environmental detection of biothreat agents</topic><topic>Environmental Monitoring</topic><topic>Enzymes</topic><topic>Forensic Medicine</topic><topic>forensic science</topic><topic>Francisella tularensis</topic><topic>Francisella tularensis - genetics</topic><topic>Francisella tularensis - isolation & purification</topic><topic>Genes</topic><topic>Pathogens</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction</topic><topic>select agent</topic><topic>Sequence Analysis, DNA</topic><topic>soil</topic><topic>Soil Microbiology</topic><topic>Yersinia pestis</topic><topic>Yersinia pestis - genetics</topic><topic>Yersinia pestis - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kuske, Cheryl R.</creatorcontrib><creatorcontrib>Barns, Susan M.</creatorcontrib><creatorcontrib>Grow, Christy C.</creatorcontrib><creatorcontrib>Merrill, Lori</creatorcontrib><creatorcontrib>Dunbar, John</creatorcontrib><creatorcontrib>Joint Genome Institute (JGI)</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Criminal Justice (Alumni)</collection><collection>OSTI.GOV</collection><jtitle>Journal of forensic sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kuske, Cheryl R.</au><au>Barns, Susan M.</au><au>Grow, Christy C.</au><au>Merrill, Lori</au><au>Dunbar, John</au><aucorp>Joint Genome Institute (JGI)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Environmental Survey for Four Pathogenic Bacteria and Closely Related Species Using Phylogenetic and Functional Genes</atitle><jtitle>Journal of forensic sciences</jtitle><addtitle>J Forensic Sci</addtitle><date>2006-05</date><risdate>2006</risdate><volume>51</volume><issue>3</issue><spage>548</spage><epage>558</epage><pages>548-558</pages><issn>0022-1198</issn><eissn>1556-4029</eissn><coden>JFSCAS</coden><abstract>Bacterial species with high DNA sequence similarity to pathogens could affect the specificity of assays designed to detect biological threat agents in environmental samples. The natural presence of four pathogenic bacteria, Bacillus anthracis, Clostridium perfringens, Francisella tularensis, and Yersinia pestis and their closely related species, was determined for a large collection of soil and aerosol samples. Polymerase chain reaction (PCR) and gene sequencing were used using group‐specific 16S rRNA primers to identify pathogens and related species, and pathogen‐specific virulence genes. Close relatives of B. anthracis (B. cereus group species) were detected in 37% of the soils and 25% of the aerosol samples. The B. anthracis protective antigen (pag) gene or a close homolog was detected in 16 of these samples. For the other three pathogen groups, the frequency of detection was much lower, and none of the samples were positive with both the phylogenetic and virulence gene primer sets.</abstract><cop>Malden, USA</cop><pub>Blackwell Publishing Inc</pub><pmid>16696701</pmid><doi>10.1111/j.1556-4029.2006.00131.x</doi><tpages>11</tpages></addata></record> |
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| subjects | aerosol Aerosols Bacillus anthracis Bacillus anthracis - genetics Bacillus anthracis - isolation & purification backgrounds Bacteria Clostridium perfringens Clostridium perfringens - genetics Clostridium perfringens - isolation & purification Deoxyribonucleic acid DNA DNA Primers DNA, Bacterial - analysis DNA, Ribosomal - analysis environmental detection of biothreat agents Environmental Monitoring Enzymes Forensic Medicine forensic science Francisella tularensis Francisella tularensis - genetics Francisella tularensis - isolation & purification Genes Pathogens Phylogeny Polymerase Chain Reaction select agent Sequence Analysis, DNA soil Soil Microbiology Yersinia pestis Yersinia pestis - genetics Yersinia pestis - isolation & purification |
| title | Environmental Survey for Four Pathogenic Bacteria and Closely Related Species Using Phylogenetic and Functional Genes |
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