Acoustic deposition with NIMS as a high-throughput enzyme activity assay
Mass spectrometry (MS)-based enzyme assay has been shown to be a useful tool for screening enzymatic activities from environmental samples. Recently, reported approaches for high-specificity multiplexed characterization of enzymatic activities allow for providing detailed information on the range of...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2012-05, Vol.403 (3), p.707-711 |
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| container_title | Analytical and bioanalytical chemistry |
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| creator | Greving, Matthew Cheng, Xiaoliang Reindl, Wolfgang Bowen, Benjamin Deng, Kai Louie, Katherine Nyman, Michael Cohen, Joseph Singh, Anup Simmons, Blake Adams, Paul Siuzdak, Gary Northen, Trent |
| description | Mass spectrometry (MS)-based enzyme assay has been shown to be a useful tool for screening enzymatic activities from environmental samples. Recently, reported approaches for high-specificity multiplexed characterization of enzymatic activities allow for providing detailed information on the range of enzymatic products and monitoring multiple enzymatic reactions. However, the throughput has been limited by the slow liquid–liquid handling and manual analysis. This rapid communication demonstrates the integration of acoustic sample deposition with nanostructure initiator mass spectrometry (NIMS) imaging to provide reproducible measurements of multiple enzymatic reactions at a throughput that is tenfold to 100-fold faster than conventional MS-based enzyme assay. It also provides a simple means for the visualization of multiple reactions and reaction pathways. |
| doi_str_mv | 10.1007/s00216-012-5908-8 |
| format | Article |
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Recently, reported approaches for high-specificity multiplexed characterization of enzymatic activities allow for providing detailed information on the range of enzymatic products and monitoring multiple enzymatic reactions. However, the throughput has been limited by the slow liquid–liquid handling and manual analysis. This rapid communication demonstrates the integration of acoustic sample deposition with nanostructure initiator mass spectrometry (NIMS) imaging to provide reproducible measurements of multiple enzymatic reactions at a throughput that is tenfold to 100-fold faster than conventional MS-based enzyme assay. 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| source | MEDLINE; SpringerLink Journals |
| subjects | Acoustics Analytical Chemistry Aspergillus niger - enzymology Assaying Bacillus - enzymology Biochemistry Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Deposition Enzyme Assays - economics Enzyme Assays - instrumentation Enzymes Equipment Design Food Science Glycoside Hydrolases - metabolism High-Throughput Screening Assays - economics High-Throughput Screening Assays - instrumentation Laboratory Medicine Mass spectrometry Materials handling Monitoring/Environmental Analysis Multiplexing Nanostructure Nanostructures - chemistry Short Communication Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - economics Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation Time Factors Xylosidases - metabolism |
| title | Acoustic deposition with NIMS as a high-throughput enzyme activity assay |
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