Virus-Induced Gene Silencing Offers a Functional Genomics Platform for Studying Plant Cell Wall Formation
Virus-induced gene silencing （VIGS） is a powerful genetic tool for rapid assessment of plant gene functions in the post-genomic era. Here, we successfully implemented a Tobacco Rattle Virus （TRV）-based VlGS system to study functions of genes involved in either primary or secondary cell wall formatio...
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| Veröffentlicht in: | Molecular plant 2010-09, Vol.3 (5), p.818-833 |
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| container_title | Molecular plant |
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| creator | Zhu, Xiaohong Pattathil, Sivakumar Mazumder, Koushik Brehm, Amanda Hahn, Michael G. Dinesh-Kumar, S.P. Joshi, Chandrashekhar P. |
| description | Virus-induced gene silencing （VIGS） is a powerful genetic tool for rapid assessment of plant gene functions in the post-genomic era. Here, we successfully implemented a Tobacco Rattle Virus （TRV）-based VlGS system to study functions of genes involved in either primary or secondary cell wall formation in Nicotiana benthamiana plants. A 3-week post- VIGS time frame is sufficient to observe phenotypic alterations in the anatomical structure of stems and chemical composition of the primary and secondary cell walls. We used cell wall glycan-directed monoclonal antibodies to demonstrate that alteration of cell wall polymer synthesis during the secondary growth phase of VIGS plants has profound effects on the extractability of components from woody stem cell walls. Therefore, TRV-based VlGS together with cell wall component profiling methods provide a high-throughput gene discovery platform for studying plant cell wall formation from a bioenergy perspective. |
| doi_str_mv | 10.1093/mp/ssq023 |
| format | Article |
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(ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)</creatorcontrib><description>Virus-induced gene silencing （VIGS） is a powerful genetic tool for rapid assessment of plant gene functions in the post-genomic era. Here, we successfully implemented a Tobacco Rattle Virus （TRV）-based VlGS system to study functions of genes involved in either primary or secondary cell wall formation in Nicotiana benthamiana plants. A 3-week post- VIGS time frame is sufficient to observe phenotypic alterations in the anatomical structure of stems and chemical composition of the primary and secondary cell walls. We used cell wall glycan-directed monoclonal antibodies to demonstrate that alteration of cell wall polymer synthesis during the secondary growth phase of VIGS plants has profound effects on the extractability of components from woody stem cell walls. Therefore, TRV-based VlGS together with cell wall component profiling methods provide a high-throughput gene discovery platform for studying plant cell wall formation from a bioenergy perspective.</description><identifier>ISSN: 1674-2052</identifier><identifier>EISSN: 1752-9867</identifier><identifier>DOI: 10.1093/mp/ssq023</identifier><identifier>PMID: 20522525</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Cell Wall - genetics ; Cell Wall - metabolism ; Cells ; cellulose ; Enzyme-Linked Immunosorbent Assay ; Enzymes ; Gene Expression Regulation, Plant - genetics ; Gene Expression Regulation, Plant - physiology ; Gene Silencing - physiology ; Genes ; Genomics ; lignin ; Nicotiana ; Nicotiana - cytology ; Nicotiana - genetics ; Plant cell wall ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Viruses - genetics ; Plant Viruses - physiology ; Proteins ; VIGS ; xylan ; 功能基因组学 ; 化学成分 ; 后基因组时代 ; 基因沉默 ; 平台 ; 植物细胞壁 ; 烟草脆裂病毒 ; 病毒诱导</subject><ispartof>Molecular plant, 2010-09, Vol.3 (5), p.818-833</ispartof><rights>2010 The Authors. All rights reserved.</rights><rights>The Author 2010. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPP and IPPE, SIBS, CAS.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-23b6f711b90b5e7df5f0f696d202e92834e85f2c839ea85ce7dfe3af4ed3656a3</citedby><cites>FETCH-LOGICAL-c445t-23b6f711b90b5e7df5f0f696d202e92834e85f2c839ea85ce7dfe3af4ed3656a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/90143B/90143B.jpg</thumbnail><link.rule.ids>230,315,781,785,886,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20522525$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/1152121$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhu, Xiaohong</creatorcontrib><creatorcontrib>Pattathil, Sivakumar</creatorcontrib><creatorcontrib>Mazumder, Koushik</creatorcontrib><creatorcontrib>Brehm, Amanda</creatorcontrib><creatorcontrib>Hahn, Michael G.</creatorcontrib><creatorcontrib>Dinesh-Kumar, S.P.</creatorcontrib><creatorcontrib>Joshi, Chandrashekhar P.</creatorcontrib><creatorcontrib>Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)</creatorcontrib><title>Virus-Induced Gene Silencing Offers a Functional Genomics Platform for Studying Plant Cell Wall Formation</title><title>Molecular plant</title><addtitle>Molecular Plant</addtitle><description>Virus-induced gene silencing （VIGS） is a powerful genetic tool for rapid assessment of plant gene functions in the post-genomic era. Here, we successfully implemented a Tobacco Rattle Virus （TRV）-based VlGS system to study functions of genes involved in either primary or secondary cell wall formation in Nicotiana benthamiana plants. A 3-week post- VIGS time frame is sufficient to observe phenotypic alterations in the anatomical structure of stems and chemical composition of the primary and secondary cell walls. We used cell wall glycan-directed monoclonal antibodies to demonstrate that alteration of cell wall polymer synthesis during the secondary growth phase of VIGS plants has profound effects on the extractability of components from woody stem cell walls. Therefore, TRV-based VlGS together with cell wall component profiling methods provide a high-throughput gene discovery platform for studying plant cell wall formation from a bioenergy perspective.</description><subject>Cell Wall - genetics</subject><subject>Cell Wall - metabolism</subject><subject>Cells</subject><subject>cellulose</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Enzymes</subject><subject>Gene Expression Regulation, Plant - genetics</subject><subject>Gene Expression Regulation, Plant - physiology</subject><subject>Gene Silencing - physiology</subject><subject>Genes</subject><subject>Genomics</subject><subject>lignin</subject><subject>Nicotiana</subject><subject>Nicotiana - cytology</subject><subject>Nicotiana - genetics</subject><subject>Plant cell wall</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Viruses - genetics</subject><subject>Plant Viruses - physiology</subject><subject>Proteins</subject><subject>VIGS</subject><subject>xylan</subject><subject>功能基因组学</subject><subject>化学成分</subject><subject>后基因组时代</subject><subject>基因沉默</subject><subject>平台</subject><subject>植物细胞壁</subject><subject>烟草脆裂病毒</subject><subject>病毒诱导</subject><issn>1674-2052</issn><issn>1752-9867</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0U1rFTEUBuAgii21C_-ABF1IF2PzPTNLuXjbQqGFVl2GTOakjc4k9yYzhf77ZpirC3GThMOTQ05ehN5T8oWSlp-Pu_Oc94TxV-iY1pJVbaPq1-WsalExItkROs3Zd0VQyhQRb9HRUmaSyWPkf_g05-oq9LOFHl9AAHznBwjWhwd84xykjA3ezsFOPgYzLCSO3mZ8O5jJxTTisuC7ae6flyulGia8gWHAP01ZtkWY5eo79MaZIcPpYT9B37ff7jeX1fXNxdXm63VlhZBTxXinXE1p15JOQt076YhTreoZYdCyhgtopGO24S2YRtqFADdOQM-VVIafoI9r35gnr7P1E9hHG0MAO2lKJaOMFvR5RbsU9zPkSY8-2_JoEyDOWddS0rpWjSzy0z_yV5xT-YisKWGEtKpRrKizVdkUc07g9C750aTngvQSkx53eo2p2A-HjnM3Qv9X_gmlAL4CKN_05CEtU5REoPdpGaKP_r9tD3Pbxxge9iUL3Rn725UwNZdKCC44fwGZoqrT</recordid><startdate>20100901</startdate><enddate>20100901</enddate><creator>Zhu, Xiaohong</creator><creator>Pattathil, Sivakumar</creator><creator>Mazumder, Koushik</creator><creator>Brehm, Amanda</creator><creator>Hahn, Michael G.</creator><creator>Dinesh-Kumar, S.P.</creator><creator>Joshi, Chandrashekhar P.</creator><general>Elsevier Inc</general><general>Cell Press</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W95</scope><scope>~WA</scope><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20100901</creationdate><title>Virus-Induced Gene Silencing Offers a Functional Genomics Platform for Studying Plant Cell Wall Formation</title><author>Zhu, Xiaohong ; Pattathil, Sivakumar ; Mazumder, Koushik ; Brehm, Amanda ; Hahn, Michael G. ; Dinesh-Kumar, S.P. ; Joshi, Chandrashekhar P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-23b6f711b90b5e7df5f0f696d202e92834e85f2c839ea85ce7dfe3af4ed3656a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Cell Wall - genetics</topic><topic>Cell Wall - metabolism</topic><topic>Cells</topic><topic>cellulose</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Enzymes</topic><topic>Gene Expression Regulation, Plant - genetics</topic><topic>Gene Expression Regulation, Plant - physiology</topic><topic>Gene Silencing - physiology</topic><topic>Genes</topic><topic>Genomics</topic><topic>lignin</topic><topic>Nicotiana</topic><topic>Nicotiana - cytology</topic><topic>Nicotiana - genetics</topic><topic>Plant cell wall</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Viruses - genetics</topic><topic>Plant Viruses - physiology</topic><topic>Proteins</topic><topic>VIGS</topic><topic>xylan</topic><topic>功能基因组学</topic><topic>化学成分</topic><topic>后基因组时代</topic><topic>基因沉默</topic><topic>平台</topic><topic>植物细胞壁</topic><topic>烟草脆裂病毒</topic><topic>病毒诱导</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhu, Xiaohong</creatorcontrib><creatorcontrib>Pattathil, Sivakumar</creatorcontrib><creatorcontrib>Mazumder, Koushik</creatorcontrib><creatorcontrib>Brehm, Amanda</creatorcontrib><creatorcontrib>Hahn, Michael G.</creatorcontrib><creatorcontrib>Dinesh-Kumar, S.P.</creatorcontrib><creatorcontrib>Joshi, Chandrashekhar P.</creatorcontrib><creatorcontrib>Oak Ridge National Lab. 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A 3-week post- VIGS time frame is sufficient to observe phenotypic alterations in the anatomical structure of stems and chemical composition of the primary and secondary cell walls. We used cell wall glycan-directed monoclonal antibodies to demonstrate that alteration of cell wall polymer synthesis during the secondary growth phase of VIGS plants has profound effects on the extractability of components from woody stem cell walls. Therefore, TRV-based VlGS together with cell wall component profiling methods provide a high-throughput gene discovery platform for studying plant cell wall formation from a bioenergy perspective.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>20522525</pmid><doi>10.1093/mp/ssq023</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Molecular plant, 2010-09, Vol.3 (5), p.818-833 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Cell Wall - genetics Cell Wall - metabolism Cells cellulose Enzyme-Linked Immunosorbent Assay Enzymes Gene Expression Regulation, Plant - genetics Gene Expression Regulation, Plant - physiology Gene Silencing - physiology Genes Genomics lignin Nicotiana Nicotiana - cytology Nicotiana - genetics Plant cell wall Plant Proteins - genetics Plant Proteins - metabolism Plant Viruses - genetics Plant Viruses - physiology Proteins VIGS xylan 功能基因组学 化学成分 后基因组时代 基因沉默 平台 植物细胞壁 烟草脆裂病毒 病毒诱导 |
| title | Virus-Induced Gene Silencing Offers a Functional Genomics Platform for Studying Plant Cell Wall Formation |
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