Defining cell culture conditions to improve human norovirus infectivity assays

Significant difficulties remain for determining whether human noroviruses (hNoV) recovered from water, food, and environmental samples are infectious. Three-dimensional (3-D) tissue culture of human intestinal cells has shown promise in developing an infectivity assay, but reproducibility, even with...

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Veröffentlicht in:	Water science and technology 2013-01, Vol.67 (4), p.863-868
Hauptverfasser:	STRAUB, T. M, HUTCHISON, J. R, BARTHOLOMEW, R. A, VALDEZ, C. O, VALENTINE, N. B, DOHNALKOVA, A, OZANICH, R. M, BRUCKNER-LEA, C. J
Format:	Artikel
Sprache:	eng
Schlagworte:	Applied sciences Assaying Brushes C2BBe1 cells Caco-2 Cells Cell culture Cell Culture Techniques Cells DNA Environmental Microbiology Environmental Molecular Sciences Laboratory Exact sciences and technology Humans Infectivity Intestines Large intestine Microscopy nfectivity Norovirus Norovirus - pathogenicity noroviruses Nucleic acids Nucleotide sequence PCR Phenotypes Pollution Polymerase chain reaction qRT-PCR Reproducibility Reverse transcription Ribonucleic acid RNA three-dimensional cell culture Tissue Tissue culture Transcription Viruses Water treatment and pollution
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