Multiplexed Electrochemical Immunoassay of Phosphorylated Proteins Based on Enzyme-Functionalized Gold Nanorod Labels and Electric Field-Driven Acceleration

A multiplexed electrochemical immunoassay integrating enzyme amplification and electric field-driven strategy was developed for fast and sensitive quantification of phosphorylated p53 at Ser392 (phospho-p53392), Ser15 (phospho-p5315), Ser46 (phospho-p5346), and total p53 simultaneously. The disposab...

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Veröffentlicht in:	Analytical chemistry (Washington) 2011-09, Vol.83 (17), p.6580-6585
Hauptverfasser:	Du, Dan, Wang, Jun, Lu, Donglai, Dohnalkova, Alice, Lin, Yuehe
Format:	Artikel
Sprache:	eng
Schlagworte:	60 APPLIED LIFE SCIENCES ACCELERATION AMPLIFICATION Analytical chemistry Antibodies, Immobilized - immunology BASIC BIOLOGICAL SCIENCES Chemistry DETECTION Electric fields electrochemical immunoassay Electrochemical methods Electrochemical Techniques - methods ELECTRODES Environmental Molecular Sciences Laboratory Enzyme-Linked Immunosorbent Assay - methods ENZYMES Enzymes, Immobilized - chemistry Enzymes, Immobilized - metabolism Exact sciences and technology General, instrumentation GOLD Gold - chemistry Horseradish Peroxidase - chemistry Horseradish Peroxidase - metabolism Humans HYDROGEN PEROXIDE Hydrogen Peroxide - chemistry IMMUNOASSAY Immunoassay - methods Miscellaneous Nanoparticles Nanotubes - chemistry PEROXIDASES Phosphopeptides - analysis Phosphopeptides - blood phosphorylated proteins Phosphorylation PROTEINS SENSITIVITY SENSORS THIONINE Tumor Suppressor Protein p53 - chemistry
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