Ca2+ entry through reverse Na+/Ca2+ exchanger in NCI-H716, glucagon-like peptide-1 secreting cells
Glucagon like peptide-1 (GLP-1) released from enteroendocine L-cells in the intestine has incretin effects due to its ability to amplify glucose-dependent insulin secretion. Promotion of an endogenous release of GLP-1 is one of therapeutic targets for type 2 diabetes mellitus. Although the secretion...
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Veröffentlicht in: | The Korean journal of physiology & pharmacology 2022, 26(3), , pp.219-225 |
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Sprache: | eng |
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Zusammenfassung: | Glucagon like peptide-1 (GLP-1) released from enteroendocine L-cells in the intestine has incretin effects due to its ability to amplify glucose-dependent insulin secretion. Promotion of an endogenous release of GLP-1 is one of therapeutic targets for type 2 diabetes mellitus. Although the secretion of GLP-1 in response to nutrient or neural stimuli can be triggered by cytosolic Ca
2+
elevation, the stimulus-secretion pathway is not completely understood yet. Therefore, the aim of this study was to investigate the role of reverse Na
+
/Ca
2+
exchanger (rNCX) in Ca
2+
entry induced by muscarinic stimulation in NCI-H716 cells, a human enteroendocrine GLP-1 secreting cell line. Intracellular Ca
2+
was repetitively oscillated by the perfusion of carbamylcholine (CCh), a muscarinic agonist. The oscillation of cytosolic Ca
2+
was ceased by substituting extracellular Na
+
with Li
+
or NMG
+
. KB-R7943, a specific rNCX blocker, completely diminished CCh-induced cytosolic Ca
2+
oscillation. Type 1 Na
+
/Ca
2+
exchanger (NCX
1
) proteins were expressed in NCI-H716 cells. These results suggest that rNCX might play a crucial role in Ca
2+
entry induced by cholinergic stimulation in NCI-H716 cells, a GLP-1 secreting cell line. |
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ISSN: | 1226-4512 2093-3827 |
DOI: | 10.4196/kjpp.2022.26.3.219 |