Correlation analysis for non-invasive quantitative monitoring of biological activity of recombinant enzyme using green fluorescence protein in Escherichia coli under various culture environments

sion systems. Previously, we demonstrated that green fluorescent protein (GFP) as a fusion partner is successfully tooledfor facile, in vivo, and non-invasive quantification of target enzyme levels based on a linear relationship betwen GFPfluorescence and enzyme (chloramphenicol acetyltransferase; CAT) activity. Here, we investigated the effects of cultureenvironmental variations (initial glucose amount, surface aeration, and inducer concentration) on correlation betweenGFP fluorescence and CAT activity, and established a general linear correlation as a unique criterion for quantitativemonitoring of CAT biological activity. This general correlation for GFP fusion strategy can be applied for non-invasiveand on-line monitoring of recombinant enzyme production under various culture conditions without further experi-mental calibrations. KCI Citation Count: 4