Paenibacillus seodonensis sp. nov., isolated from a plant of the genus Campanula

Strain DCT-19 T , representing a Gram-stain-positive, rodshaped, aerobic bacterium, was isolated from a native plant belonging to the genus Campanula on Dokdo, the Republic of Korea. Comparative analysis of the 16S rRNA gene sequence showed that this strain was closely related to Paenibacillus amylolyticus NRRL NRS-290 T (98.6%, 16S rRNA gene sequence similarity), Paenibacillus tundrae A10b T (98.1%), and Paenibacillus xylanexedens NRRL B-51090 T (97.6%). DNADNA hybridization indicated that this strain had relatively low levels of DNA-DNA relatedness with P. amylolyticus NRRL NRS-290 T (30.0%), P. xylanexedens NRRL B-51090 T (29.0%), and P. tundrae A10b T (24.5%). Additionally, the genomic DNA G + C content of DCT-19 T was 44.8%. The isolated strain grew at pH 6.0–8.0 (optimum, pH 7.0), 0–4% (w/v) NaCl (optimum, 0%), and a temperature of 15–45°C (optimum 25–30°C). The sole respiratory quinone in the strain was menaquinone-7, and the predominant fatty acids were C 15:0 anteiso, C 16:0 iso, and C 16:0 . In addition, the major polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. Based on its phenotypic properties, genotypic distinctiveness, and chemotaxonomic features, strain DCT-19 T is proposed as a novel species in the genus Paenibacillus , for which the name Paenibacillus seodonensis sp. nov. is proposed (=KCTC 43009 T =LMG 30888 T ). The type strain of Paenibacillus seodonensis is DCT-19 T .