대왕범바리(Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂) 수정란의장거리 수송을 위한 염분, 수온 및 발생단계에 따른 생존율 및 부화율

We examined the effect of salinity and water temperature on hatching and survival rates of fertilized eggs of hybrid grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂) at different developmental stages, determining optimal conditions for their long-distance transportation. Deformities and hatching rates of fertilized grouper eggs were observed at salinities of 24, 27, 30, 33, 36, and 39 psu. The optimal salinity was determined to be 36 psu, with a survival rate of 70.0 ± 2.0%. Transportation experiments at 36 psu were conducted at water temperatures of 21, 24, 27, and 30oC, different developmental stages such as morula, 5-myomere, and tail beating for hatching and survival rates. The optimal water temperature and developmental stage for transporatation were 30oC and tail beating stage and those hatching rates were 50.6 ± 1.9% and 86.3 ± 1.3%, respectively. At 21oC, the survival rate by lowest. Therefore, the hybrid grouper fertilized eggs (E. fuscoguttatus ♀ × E. lanceolatus ♂) can be most efficiently produced under long-distance transportation conditions during the tail beating stage and at a water temperature of 30oC. KCI Citation Count: 0