Identification of PCR-based markers flanking the recessive LMV resistance gene mo1 in an intraspecific cross in lettuce
Molecular markers flanking the recessive resistance gene mo1 were identified following analysis of two segregating populations. Generation of a population from a crisphead x crisphead cross of Lactuca sativa that segregated for resistance conferred by the mo1(2) allele, but not morphological traits,...
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| Veröffentlicht in: | Genome 1999-10, Vol.42 (5), p.982-986 |
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| container_title | Genome |
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| creator | Irwin, S.V Kesseli, R.V Waycott, W Ryder, E.J Cho, J.J Michelmore, R.W |
| description | Molecular markers flanking the recessive resistance gene mo1 were identified following analysis of two segregating populations. Generation of a population from a crisphead x crisphead cross of Lactuca sativa that segregated for resistance conferred by the mo1(2) allele, but not morphological traits, was required for accurate mapping of the gene. Resistance was best assessed by symptom development after inoculating F(3) families with the common pathotype II isolate of lettuce mosaic virus (LMV). Bulked segregant analysis for RAPD (random amplified polymorphic DNA) markers using one population, followed by linkage analysis using another, identified markers on either side of the mo1 gene, approximately 8 cM apart. These markers will be useful for more efficient introgression of this resistance gene into additional lettuce cultivars. |
| doi_str_mv | 10.1139/g99-055 |
| format | Article |
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Generation of a population from a crisphead x crisphead cross of Lactuca sativa that segregated for resistance conferred by the mo1(2) allele, but not morphological traits, was required for accurate mapping of the gene. Resistance was best assessed by symptom development after inoculating F(3) families with the common pathotype II isolate of lettuce mosaic virus (LMV). Bulked segregant analysis for RAPD (random amplified polymorphic DNA) markers using one population, followed by linkage analysis using another, identified markers on either side of the mo1 gene, approximately 8 cM apart. These markers will be useful for more efficient introgression of this resistance gene into additional lettuce cultivars.</description><identifier>ISSN: 0831-2796</identifier><identifier>EISSN: 1480-3321</identifier><identifier>DOI: 10.1139/g99-055</identifier><identifier>CODEN: GENOE3</identifier><language>eng</language><publisher>Ottawa, Canada: NRC Research Press</publisher><subject>alleles ; chromosome mapping ; Cultivars ; disease course ; disease resistance ; Diseases & pests ; Flowers & plants ; genes ; genetic markers ; genetic resistance ; genetic techniques and protocols ; Genetics ; hybrids ; Lactuca sativa ; Lettuce ; Lettuce mosaic virus ; linkage (genetics) ; mo1 gene ; polymerase chain reaction ; R&D ; random amplified polymorphic DNA technique ; recessive genes ; Research & development ; segregation ; symptoms ; Vegetables</subject><ispartof>Genome, 1999-10, Vol.42 (5), p.982-986</ispartof><rights>Copyright National Research Council of Canada Oct 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c286t-68853cd95628e7a64d70c48423b901d985775b496df288483d526b9e4c84fb913</citedby><cites>FETCH-LOGICAL-c286t-68853cd95628e7a64d70c48423b901d985775b496df288483d526b9e4c84fb913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://cdnsciencepub.com/doi/pdf/10.1139/g99-055$$EPDF$$P50$$Gnrcresearch$$H</linktopdf><linktohtml>$$Uhttps://cdnsciencepub.com/doi/full/10.1139/g99-055$$EHTML$$P50$$Gnrcresearch$$H</linktohtml><link.rule.ids>314,777,781,2919,27905,27906,64407,64985</link.rule.ids></links><search><creatorcontrib>Irwin, S.V</creatorcontrib><creatorcontrib>Kesseli, R.V</creatorcontrib><creatorcontrib>Waycott, W</creatorcontrib><creatorcontrib>Ryder, E.J</creatorcontrib><creatorcontrib>Cho, J.J</creatorcontrib><creatorcontrib>Michelmore, R.W</creatorcontrib><title>Identification of PCR-based markers flanking the recessive LMV resistance gene mo1 in an intraspecific cross in lettuce</title><title>Genome</title><addtitle>Génome</addtitle><description>Molecular markers flanking the recessive resistance gene mo1 were identified following analysis of two segregating populations. Generation of a population from a crisphead x crisphead cross of Lactuca sativa that segregated for resistance conferred by the mo1(2) allele, but not morphological traits, was required for accurate mapping of the gene. Resistance was best assessed by symptom development after inoculating F(3) families with the common pathotype II isolate of lettuce mosaic virus (LMV). Bulked segregant analysis for RAPD (random amplified polymorphic DNA) markers using one population, followed by linkage analysis using another, identified markers on either side of the mo1 gene, approximately 8 cM apart. These markers will be useful for more efficient introgression of this resistance gene into additional lettuce cultivars.</description><subject>alleles</subject><subject>chromosome mapping</subject><subject>Cultivars</subject><subject>disease course</subject><subject>disease resistance</subject><subject>Diseases & pests</subject><subject>Flowers & plants</subject><subject>genes</subject><subject>genetic markers</subject><subject>genetic resistance</subject><subject>genetic techniques and protocols</subject><subject>Genetics</subject><subject>hybrids</subject><subject>Lactuca sativa</subject><subject>Lettuce</subject><subject>Lettuce mosaic virus</subject><subject>linkage (genetics)</subject><subject>mo1 gene</subject><subject>polymerase chain reaction</subject><subject>R&D</subject><subject>random amplified polymorphic DNA technique</subject><subject>recessive genes</subject><subject>Research & development</subject><subject>segregation</subject><subject>symptoms</subject><subject>Vegetables</subject><issn>0831-2796</issn><issn>1480-3321</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp90F1LHDEUBuBQFLpV6U9o6IVCYWq-Zia5lEWrsEVptbchkzlZsx-ZNcko_nuzrldCvckh5OGcnBehr5T8pJSr07lSFanrT2hChSQV54zuoQmRnFasVc1n9CWlBSGUcEUn6Omqh5C989ZkPwQ8OHwz_VN1JkGP1yYuISbsViYsfZjjfA84goWU_CPg2e9_5ZZ8yiZYwHMIgNcDxT5gE8qZo0kbsNvm2MYhpe3LCnIeLRyifWdWCY7e6gG6uzi_nV5Ws-tfV9OzWWWZbHLVSFlz26u6YRJa04i-JVZIwXinCO2VrNu27oRqesekFJL3NWs6BcJK4TpF-QE63vXdxOFhhJT12icLq7IRDGPStBW0VpIU-P0dXAxjDOVvmjEilChDCjrZodd1Iji9ib6k9Kwp0dv0dUlfl_SL_LGTIdqSEZho7z_Ax__Hb0hvelfgtx10ZtBmHn3Sd38ZoZwwxZiijL8AT52a7Q</recordid><startdate>19991001</startdate><enddate>19991001</enddate><creator>Irwin, 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gene</topic><topic>polymerase chain reaction</topic><topic>R&D</topic><topic>random amplified polymorphic DNA technique</topic><topic>recessive genes</topic><topic>Research & development</topic><topic>segregation</topic><topic>symptoms</topic><topic>Vegetables</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Irwin, S.V</creatorcontrib><creatorcontrib>Kesseli, R.V</creatorcontrib><creatorcontrib>Waycott, W</creatorcontrib><creatorcontrib>Ryder, E.J</creatorcontrib><creatorcontrib>Cho, J.J</creatorcontrib><creatorcontrib>Michelmore, R.W</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health 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| subjects | alleles chromosome mapping Cultivars disease course disease resistance Diseases & pests Flowers & plants genes genetic markers genetic resistance genetic techniques and protocols Genetics hybrids Lactuca sativa Lettuce Lettuce mosaic virus linkage (genetics) mo1 gene polymerase chain reaction R&D random amplified polymorphic DNA technique recessive genes Research & development segregation symptoms Vegetables |
| title | Identification of PCR-based markers flanking the recessive LMV resistance gene mo1 in an intraspecific cross in lettuce |
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