Expression of nicotinic acetylcholine receptor subunit α9 in type Ⅱ vestibular hair cells of rats
Aim： To explore the cell specific existence of α9 AChR in the vestibular type Ⅱ hair cells （VHC Ⅱ） of rats. Methods： To detect the expression of α9 AChR messenger RNA （mRNA） in the vestibular endorgans and single VHC Ⅱ of rats by using the reverse transcription polymerase chain reaction （RT-PCR） tec...
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| Veröffentlicht in: | Acta pharmacologica Sinica 2006-11, Vol.27 (11), p.1509-1514 |
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| container_title | Acta pharmacologica Sinica |
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| creator | Wei-jia KONG Hua-mao CHENG Paul van CAUWENBERGE |
| description | Aim： To explore the cell specific existence of α9 AChR in the vestibular type Ⅱ hair cells （VHC Ⅱ） of rats. Methods： To detect the expression of α9 AChR messenger RNA （mRNA） in the vestibular endorgans and single VHC Ⅱ of rats by using the reverse transcription polymerase chain reaction （RT-PCR） technique and the single cell RT-PCR technique, respectively. Results： It was shown that α9 AChR mRNA was detected in the vestibular endorgans. By using single-cell RT-PCR, mRNA encoding α9 AChR was also detected in the VHC Ⅱ of the rats. Sequence analysis of the PCR products confirmed identity to corresponding cDNA sequence in the predicted region. Condusion： We established a method which could effectively detect the cell specific expression of mRNA in an individual VHC. Present data confirm that α9 AChR mRNA is expressed in the VHC Ⅱ of rats and indicates that α9 AChR may function as a mediator of efferent cholinergic signaling in mammalian VHC. |
| doi_str_mv | 10.1111/j.1745-7254.2006.00423.x |
| format | Article |
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Methods： To detect the expression of α9 AChR messenger RNA （mRNA） in the vestibular endorgans and single VHC Ⅱ of rats by using the reverse transcription polymerase chain reaction （RT-PCR） technique and the single cell RT-PCR technique, respectively. Results： It was shown that α9 AChR mRNA was detected in the vestibular endorgans. By using single-cell RT-PCR, mRNA encoding α9 AChR was also detected in the VHC Ⅱ of the rats. Sequence analysis of the PCR products confirmed identity to corresponding cDNA sequence in the predicted region. Condusion： We established a method which could effectively detect the cell specific expression of mRNA in an individual VHC. Present data confirm that α9 AChR mRNA is expressed in the VHC Ⅱ of rats and indicates that α9 AChR may function as a mediator of efferent cholinergic signaling in mammalian VHC.</description><identifier>ISSN: 1671-4083</identifier><identifier>EISSN: 1745-7254</identifier><identifier>DOI: 10.1111/j.1745-7254.2006.00423.x</identifier><language>eng</language><subject>乙酰胆碱 ; 毛发细胞</subject><ispartof>Acta pharmacologica Sinica, 2006-11, Vol.27 (11), p.1509-1514</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/95561A/95561A.jpg</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Wei-jia KONG Hua-mao CHENG Paul van CAUWENBERGE</creatorcontrib><title>Expression of nicotinic acetylcholine receptor subunit α9 in type Ⅱ vestibular hair cells of rats</title><title>Acta pharmacologica Sinica</title><addtitle>Acta Pharmacologica Sinica</addtitle><description>Aim： To explore the cell specific existence of α9 AChR in the vestibular type Ⅱ hair cells （VHC Ⅱ） of rats. Methods： To detect the expression of α9 AChR messenger RNA （mRNA） in the vestibular endorgans and single VHC Ⅱ of rats by using the reverse transcription polymerase chain reaction （RT-PCR） technique and the single cell RT-PCR technique, respectively. Results： It was shown that α9 AChR mRNA was detected in the vestibular endorgans. By using single-cell RT-PCR, mRNA encoding α9 AChR was also detected in the VHC Ⅱ of the rats. Sequence analysis of the PCR products confirmed identity to corresponding cDNA sequence in the predicted region. Condusion： We established a method which could effectively detect the cell specific expression of mRNA in an individual VHC. 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Methods： To detect the expression of α9 AChR messenger RNA （mRNA） in the vestibular endorgans and single VHC Ⅱ of rats by using the reverse transcription polymerase chain reaction （RT-PCR） technique and the single cell RT-PCR technique, respectively. Results： It was shown that α9 AChR mRNA was detected in the vestibular endorgans. By using single-cell RT-PCR, mRNA encoding α9 AChR was also detected in the VHC Ⅱ of the rats. Sequence analysis of the PCR products confirmed identity to corresponding cDNA sequence in the predicted region. Condusion： We established a method which could effectively detect the cell specific expression of mRNA in an individual VHC. Present data confirm that α9 AChR mRNA is expressed in the VHC Ⅱ of rats and indicates that α9 AChR may function as a mediator of efferent cholinergic signaling in mammalian VHC.</abstract><doi>10.1111/j.1745-7254.2006.00423.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Acta pharmacologica Sinica, 2006-11, Vol.27 (11), p.1509-1514 |
| issn | 1671-4083 1745-7254 |
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| source | Alma/SFX Local Collection; Free Full-Text Journals in Chemistry; Wiley Blackwell Journals |
| subjects | 乙酰胆碱 毛发细胞 |
| title | Expression of nicotinic acetylcholine receptor subunit α9 in type Ⅱ vestibular hair cells of rats |
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