CBF-1 (RBP-Jk) Binds to the PTEN Promoter and Regulates PTEN Gene Expression
The PTEN gene regulates multiple signaling pathways that influence cell proliferation, survival and differentiation. Loss of PTEN expression is closely linked with oncogenesis. Little is known regarding regulation of PTEN gene expression. The PTEN promoter region has been reported and is regulated i...
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| Veröffentlicht in: | Cell cycle (Georgetown, Tex.) Tex.), 2007-01, Vol.6 (1), p.80-84 |
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| creator | Whelan, Jarrett T Forbes, Siiri L Bertrand, Fred E |
| description | The PTEN gene regulates multiple signaling pathways that influence cell proliferation, survival and differentiation. Loss of PTEN expression is closely linked with oncogenesis. Little is known regarding regulation of PTEN gene expression. The PTEN promoter region has been reported and is regulated in part by p53. In a previous study, we found that Notch-1 signaling resulted in increased PTEN protein expression. Herein, we tested the hypothesis that the PTEN gene is a direct target of Notch-1 signal transduction, through binding of the Notch-activated transcription factor CBF-1 to the PTEN minimal promoter. 293 cells expressing constitutively active Notch-1 exhibited increased PTEN gene expression and promoter transactivation. Overexpression of CBF-1 in 293 cells resulted in decreased PTEN gene expression. Mobility shift assays and supershift assays demonstrated that CBF-1 binds to the PTEN minimal promoter. These data indicate that the Notch-1 receptor pathway is a key regulator of PTEN gene transcription. |
| doi_str_mv | 10.4161/cc.6.1.3648 |
| format | Article |
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Loss of PTEN expression is closely linked with oncogenesis. Little is known regarding regulation of PTEN gene expression. The PTEN promoter region has been reported and is regulated in part by p53. In a previous study, we found that Notch-1 signaling resulted in increased PTEN protein expression. Herein, we tested the hypothesis that the PTEN gene is a direct target of Notch-1 signal transduction, through binding of the Notch-activated transcription factor CBF-1 to the PTEN minimal promoter. 293 cells expressing constitutively active Notch-1 exhibited increased PTEN gene expression and promoter transactivation. Overexpression of CBF-1 in 293 cells resulted in decreased PTEN gene expression. Mobility shift assays and supershift assays demonstrated that CBF-1 binds to the PTEN minimal promoter. These data indicate that the Notch-1 receptor pathway is a key regulator of PTEN gene transcription.</description><subject>Base Sequence</subject><subject>Binding</subject><subject>Biology</subject><subject>Bioscience</subject><subject>Calcium</subject><subject>Cancer</subject><subject>Cell</subject><subject>Cell Line</subject><subject>Cycle</subject><subject>Gene Expression Regulation - physiology</subject><subject>Humans</subject><subject>Immunoglobulin J Recombination Signal Sequence-Binding Protein - genetics</subject><subject>Immunoglobulin J Recombination Signal Sequence-Binding Protein - metabolism</subject><subject>Landes</subject><subject>Molecular Sequence Data</subject><subject>Organogenesis</subject><subject>Promoter Regions, Genetic - physiology</subject><subject>Protein Binding - genetics</subject><subject>Proteins</subject><subject>PTEN Phosphohydrolase - biosynthesis</subject><subject>PTEN Phosphohydrolase - genetics</subject><subject>PTEN Phosphohydrolase - metabolism</subject><issn>1538-4101</issn><issn>1551-4005</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkNFLwzAQh4Mobk6ffJc8iSLdkjRJm0c3tqkMHWM-hzS5abVrZ9KC_vd2dOjT3XEf3x0_hC4pGXIq6cjaoRzSYSx5eoT6VAgacULE8b6P04hTQnvoLIQPQliaKHqKejRhXFAm-mgxGc8iim9W42X09HmLx3npAq4rXL8DXq6nz3jpq21Vg8emdHgFb01hagjdbg4l4On3zkMIeVWeo5ONKQJcHOoAvc6m68lDtHiZP07uF5Ftn6wjlsgsc0aoNHGx2ySSCCm4A0OIYjEDqTLGXSKFgg1jmVRMxopxlXCjIG2HAbruvDtffTUQar3Ng4WiMCVUTdAyVTIRJG3Buw60vgrBw0bvfL41_kdTovfhaWu11FTvw2vpq4O2ybbg_tlDWi0w6oD2kIOQ5VWwOZQW_tBWZ3yd2wI65S-NNXdA</recordid><startdate>20070101</startdate><enddate>20070101</enddate><creator>Whelan, Jarrett T</creator><creator>Forbes, Siiri L</creator><creator>Bertrand, Fred E</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20070101</creationdate><title>CBF-1 (RBP-Jk) Binds to the PTEN Promoter and Regulates PTEN Gene Expression</title><author>Whelan, Jarrett T ; Forbes, Siiri L ; Bertrand, Fred E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c364t-276bbda5987d3df7605654dea009232e69b24d7659ef22b69263924974a9e8263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Base Sequence</topic><topic>Binding</topic><topic>Biology</topic><topic>Bioscience</topic><topic>Calcium</topic><topic>Cancer</topic><topic>Cell</topic><topic>Cell Line</topic><topic>Cycle</topic><topic>Gene Expression Regulation - physiology</topic><topic>Humans</topic><topic>Immunoglobulin J Recombination Signal Sequence-Binding Protein - genetics</topic><topic>Immunoglobulin J Recombination Signal Sequence-Binding Protein - metabolism</topic><topic>Landes</topic><topic>Molecular Sequence Data</topic><topic>Organogenesis</topic><topic>Promoter Regions, Genetic - physiology</topic><topic>Protein Binding - genetics</topic><topic>Proteins</topic><topic>PTEN Phosphohydrolase - biosynthesis</topic><topic>PTEN Phosphohydrolase - genetics</topic><topic>PTEN Phosphohydrolase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Whelan, Jarrett T</creatorcontrib><creatorcontrib>Forbes, Siiri L</creatorcontrib><creatorcontrib>Bertrand, Fred E</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cell cycle (Georgetown, Tex.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Whelan, Jarrett T</au><au>Forbes, Siiri L</au><au>Bertrand, Fred E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CBF-1 (RBP-Jk) Binds to the PTEN Promoter and Regulates PTEN Gene Expression</atitle><jtitle>Cell cycle (Georgetown, Tex.)</jtitle><addtitle>Cell Cycle</addtitle><date>2007-01-01</date><risdate>2007</risdate><volume>6</volume><issue>1</issue><spage>80</spage><epage>84</epage><pages>80-84</pages><issn>1538-4101</issn><eissn>1551-4005</eissn><abstract>The PTEN gene regulates multiple signaling pathways that influence cell proliferation, survival and differentiation. 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| subjects | Base Sequence Binding Biology Bioscience Calcium Cancer Cell Cell Line Cycle Gene Expression Regulation - physiology Humans Immunoglobulin J Recombination Signal Sequence-Binding Protein - genetics Immunoglobulin J Recombination Signal Sequence-Binding Protein - metabolism Landes Molecular Sequence Data Organogenesis Promoter Regions, Genetic - physiology Protein Binding - genetics Proteins PTEN Phosphohydrolase - biosynthesis PTEN Phosphohydrolase - genetics PTEN Phosphohydrolase - metabolism |
| title | CBF-1 (RBP-Jk) Binds to the PTEN Promoter and Regulates PTEN Gene Expression |
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