Characterization of the gut bacterial and viral microbiota in latent autoimmune diabetes in adults

Latent autoimmune diabetes in adults (LADA) is a heterogeneous disease characterized by autoantibodies against insulin producing pancreatic beta cells and initial lack of need for insulin treatment. The aim of the present study was to investigate if individuals with LADA have an altered gut microbio...

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Veröffentlicht in:SCIENTIFIC REPORTS 2024-04, Vol.14 (1)
Hauptverfasser: Poulsen, Casper S, Hesse, Dan, Fernandes, Gabriel R, Hansen, Tue H, Kern, Timo, Linneberg, Allan, Van Espen, Lore, Jorgensen, Torben, Nielsen, Trine, Alibegovic, Amra C, Matthijnssens, Jelle, Pedersen, Oluf, Vestergaard, Henrik, Hansen, Torben, Andersen, Mette K
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container_title SCIENTIFIC REPORTS
container_volume 14
creator Poulsen, Casper S
Hesse, Dan
Fernandes, Gabriel R
Hansen, Tue H
Kern, Timo
Linneberg, Allan
Van Espen, Lore
Jorgensen, Torben
Nielsen, Trine
Alibegovic, Amra C
Matthijnssens, Jelle
Pedersen, Oluf
Vestergaard, Henrik
Hansen, Torben
Andersen, Mette K
description Latent autoimmune diabetes in adults (LADA) is a heterogeneous disease characterized by autoantibodies against insulin producing pancreatic beta cells and initial lack of need for insulin treatment. The aim of the present study was to investigate if individuals with LADA have an altered gut microbiota relative to non-diabetic control subjects, individuals with type 1 diabetes (T1D), and individuals with type 2 diabetes (T2D). Bacterial community profiling was performed with primers targeting the variable region 4 of the 16S rRNA gene and sequenced. Amplicon sequence variants (ASVs) were generated with DADA2 and annotated to the SILVA database. The gut virome was sequenced, using a viral particle enrichment and metagenomics approach, assembled, and quantified to describe the composition of the viral community. Comparison of the bacterial alpha- and beta-diversity measures revealed that the gut bacteriome of individuals with LADA resembled that of individuals with T2D. Yet, specific genera were found to differ in abundance in individuals with LADA compared with T1D and T2D, indicating that LADA has unique taxonomical features. The virome composition reflected the stability of the most dominant order Caudovirales and the families Siphoviridae, Podoviridae, and Inoviridae, and the dominant family Microviridae. Further studies are needed to confirm these findings.
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title Characterization of the gut bacterial and viral microbiota in latent autoimmune diabetes in adults
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