Oxygen as a key parameter in in vitro dynamic and multi-compartment models to improve microbiome studies of the small intestine?

In vitro digestion and fermentation models are frequently used for human and animal research purposes. Different dynamic and multi-compartment models exist, but none have been validated with representative microbiota in the distal parts of the small intestine. We recently developed a dynamic and multi-compartment piglet model introducing microbiota in an ileum bioreactor. However, it presented discrepancies compared to in vivo data. Recommendations are available to standardize studies in this field. They target the digestion model but include elements of a fermentation model. But no recommendation is given concerning control of the atmosphere. The gastrointestinal tract is generally associated with anaerobiosis to conduct a good fermentation process. In this study, we attempted to improve the ileal microbiota of the piglet model by testing inoculation: real intestinal content vs feces; the latter being generally used for ethical and economical aspects. Results showed a positive effect of using real intestinal content. Fusobacteriia were less abundant in the model, Bacteroidia were better maintained in the colon. But for the ileum, results showed that anoxic conditions in the ileum bioreactor conditioned the microbial profile probably more than the type of inoculum itself, leading to the general conclusion that in vitro dynamic and multi-compartment models probably have to get oxygenated to improve microbiome studies of the small intestine.