Morphometric Analysis of Explant Lungs in Cystic Fibrosis
RATIONALE: After repeated cycles of lung infection and inflammation, patients with cystic fibrosis (CF) evolve to respiratory insufficiency. Although histology and imaging have provided descriptive information, a thorough morphometric analysis of end-stage CF lung disease is lacking. OBJECTIVES: To...
Gespeichert in:
Veröffentlicht in: | AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE 2016-03, Vol.193 (5), p.516-526 |
---|---|
Hauptverfasser: | , , , , , , , , , , , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 526 |
---|---|
container_issue | 5 |
container_start_page | 516 |
container_title | AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE |
container_volume | 193 |
creator | Boon, Mieke Verleden, Stijn E Bosch, Barbara Lammertyn, Elise J McDonough, John E Mai, Cindy Verschakelen, Johny Kemner-van de Corput, Mariette Tiddens, Harm A.W Proesmans, Marijke Vermeulen, Francois L Verbeken, Erik K Cooper, Joel Van Raemdonck, Dirk E Decramer, Marc Verleden, Geert M Hogg, James C Dupont, Lieven J Vanaudenaerde, Bart M De Boeck, Kris |
description | RATIONALE: After repeated cycles of lung infection and inflammation, patients with cystic fibrosis (CF) evolve to respiratory insufficiency. Although histology and imaging have provided descriptive information, a thorough morphometric analysis of end-stage CF lung disease is lacking. OBJECTIVES: To quantify the involvement of small and large airways in end-stage CF. METHODS: Multidetector computed tomography (MDCT) and micro-CT were applied to 11 air-inflated CF explanted lungs and 7 control lungs to measure, count, and describe the airway and parenchymal abnormalities in end-stage CF lungs. Selected abnormalities were further investigated with thin section histology. MEASUREMENTS AND MAIN RESULTS: On MDCT, CF explanted lungs showed an increased median (interquartile range) number (631 [511-710] vs. 344 [277-349]; P = 0.003) and size of visible airways (cumulative airway diameter 217 cm [209-250] vs. 91 cm [80-105]; P < 0.001) compared with controls. Airway obstruction was seen, starting from generation 6 and increasing to 40 to 50% of airways from generation 9 onward. Micro-CT showed that the total number of terminal bronchioles was decreased (2.9/ml [2.6-4.4] vs. 5.3/ml [4.8-5.7]; P < 0.001); 49% were obstructed, and the cross-sectional area of the open terminal bronchioles was reduced (0.093 mm(2) [0.084-0.123] vs. 0.179 mm(2) [0.140-0.196]; P < 0.001). On micro-CT, 41% of the obstructed airways reopened more distally. This remodeling was confirmed on histological analysis. Parenchymal changes were also seen, mostly in a patchy and peribronchiolar distribution. CONCLUSIONS: Extensive changes of dilatation and obstruction in nearly all airway generations were observed in end-stage CF lung disease. |
format | Article |
fullrecord | <record><control><sourceid>kuleuven</sourceid><recordid>TN_cdi_kuleuven_dspace_123456789_516106</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>123456789_516106</sourcerecordid><originalsourceid>FETCH-kuleuven_dspace_123456789_5161063</originalsourceid><addsrcrecordid>eNpjYeA0NDA31jUxsYzgYOAqLs4yMDA0sjA04GSw9M0vKsjIz00tKcpMVnDMS8ypLM4sVshPU3CtKMhJzCtR8CnNSy9WyMxTcK4sLgGqcctMKsoHquFhYE1LzClO5YXS3Azqbq4hzh662aU5qaVlqXnxKcUFicmp8YZGxiamZuYWlvGmhmaGBmbGpKjUJk5lfElFiTEAxytEcQ</addsrcrecordid><sourcetype>Institutional Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Morphometric Analysis of Explant Lungs in Cystic Fibrosis</title><source>Lirias (KU Leuven Association)</source><source>Journals@Ovid Complete</source><source>American Thoracic Society (ATS) Journals Online</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Boon, Mieke ; Verleden, Stijn E ; Bosch, Barbara ; Lammertyn, Elise J ; McDonough, John E ; Mai, Cindy ; Verschakelen, Johny ; Kemner-van de Corput, Mariette ; Tiddens, Harm A.W ; Proesmans, Marijke ; Vermeulen, Francois L ; Verbeken, Erik K ; Cooper, Joel ; Van Raemdonck, Dirk E ; Decramer, Marc ; Verleden, Geert M ; Hogg, James C ; Dupont, Lieven J ; Vanaudenaerde, Bart M ; De Boeck, Kris</creator><creatorcontrib>Boon, Mieke ; Verleden, Stijn E ; Bosch, Barbara ; Lammertyn, Elise J ; McDonough, John E ; Mai, Cindy ; Verschakelen, Johny ; Kemner-van de Corput, Mariette ; Tiddens, Harm A.W ; Proesmans, Marijke ; Vermeulen, Francois L ; Verbeken, Erik K ; Cooper, Joel ; Van Raemdonck, Dirk E ; Decramer, Marc ; Verleden, Geert M ; Hogg, James C ; Dupont, Lieven J ; Vanaudenaerde, Bart M ; De Boeck, Kris</creatorcontrib><description>RATIONALE: After repeated cycles of lung infection and inflammation, patients with cystic fibrosis (CF) evolve to respiratory insufficiency. Although histology and imaging have provided descriptive information, a thorough morphometric analysis of end-stage CF lung disease is lacking. OBJECTIVES: To quantify the involvement of small and large airways in end-stage CF. METHODS: Multidetector computed tomography (MDCT) and micro-CT were applied to 11 air-inflated CF explanted lungs and 7 control lungs to measure, count, and describe the airway and parenchymal abnormalities in end-stage CF lungs. Selected abnormalities were further investigated with thin section histology. MEASUREMENTS AND MAIN RESULTS: On MDCT, CF explanted lungs showed an increased median (interquartile range) number (631 [511-710] vs. 344 [277-349]; P = 0.003) and size of visible airways (cumulative airway diameter 217 cm [209-250] vs. 91 cm [80-105]; P < 0.001) compared with controls. Airway obstruction was seen, starting from generation 6 and increasing to 40 to 50% of airways from generation 9 onward. Micro-CT showed that the total number of terminal bronchioles was decreased (2.9/ml [2.6-4.4] vs. 5.3/ml [4.8-5.7]; P < 0.001); 49% were obstructed, and the cross-sectional area of the open terminal bronchioles was reduced (0.093 mm(2) [0.084-0.123] vs. 0.179 mm(2) [0.140-0.196]; P < 0.001). On micro-CT, 41% of the obstructed airways reopened more distally. This remodeling was confirmed on histological analysis. Parenchymal changes were also seen, mostly in a patchy and peribronchiolar distribution. CONCLUSIONS: Extensive changes of dilatation and obstruction in nearly all airway generations were observed in end-stage CF lung disease.</description><identifier>ISSN: 1073-449X</identifier><language>eng</language><publisher>AMER THORACIC SOC</publisher><ispartof>AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2016-03, Vol.193 (5), p.516-526</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,315,780,784,27860</link.rule.ids></links><search><creatorcontrib>Boon, Mieke</creatorcontrib><creatorcontrib>Verleden, Stijn E</creatorcontrib><creatorcontrib>Bosch, Barbara</creatorcontrib><creatorcontrib>Lammertyn, Elise J</creatorcontrib><creatorcontrib>McDonough, John E</creatorcontrib><creatorcontrib>Mai, Cindy</creatorcontrib><creatorcontrib>Verschakelen, Johny</creatorcontrib><creatorcontrib>Kemner-van de Corput, Mariette</creatorcontrib><creatorcontrib>Tiddens, Harm A.W</creatorcontrib><creatorcontrib>Proesmans, Marijke</creatorcontrib><creatorcontrib>Vermeulen, Francois L</creatorcontrib><creatorcontrib>Verbeken, Erik K</creatorcontrib><creatorcontrib>Cooper, Joel</creatorcontrib><creatorcontrib>Van Raemdonck, Dirk E</creatorcontrib><creatorcontrib>Decramer, Marc</creatorcontrib><creatorcontrib>Verleden, Geert M</creatorcontrib><creatorcontrib>Hogg, James C</creatorcontrib><creatorcontrib>Dupont, Lieven J</creatorcontrib><creatorcontrib>Vanaudenaerde, Bart M</creatorcontrib><creatorcontrib>De Boeck, Kris</creatorcontrib><title>Morphometric Analysis of Explant Lungs in Cystic Fibrosis</title><title>AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE</title><description>RATIONALE: After repeated cycles of lung infection and inflammation, patients with cystic fibrosis (CF) evolve to respiratory insufficiency. Although histology and imaging have provided descriptive information, a thorough morphometric analysis of end-stage CF lung disease is lacking. OBJECTIVES: To quantify the involvement of small and large airways in end-stage CF. METHODS: Multidetector computed tomography (MDCT) and micro-CT were applied to 11 air-inflated CF explanted lungs and 7 control lungs to measure, count, and describe the airway and parenchymal abnormalities in end-stage CF lungs. Selected abnormalities were further investigated with thin section histology. MEASUREMENTS AND MAIN RESULTS: On MDCT, CF explanted lungs showed an increased median (interquartile range) number (631 [511-710] vs. 344 [277-349]; P = 0.003) and size of visible airways (cumulative airway diameter 217 cm [209-250] vs. 91 cm [80-105]; P < 0.001) compared with controls. Airway obstruction was seen, starting from generation 6 and increasing to 40 to 50% of airways from generation 9 onward. Micro-CT showed that the total number of terminal bronchioles was decreased (2.9/ml [2.6-4.4] vs. 5.3/ml [4.8-5.7]; P < 0.001); 49% were obstructed, and the cross-sectional area of the open terminal bronchioles was reduced (0.093 mm(2) [0.084-0.123] vs. 0.179 mm(2) [0.140-0.196]; P < 0.001). On micro-CT, 41% of the obstructed airways reopened more distally. This remodeling was confirmed on histological analysis. Parenchymal changes were also seen, mostly in a patchy and peribronchiolar distribution. CONCLUSIONS: Extensive changes of dilatation and obstruction in nearly all airway generations were observed in end-stage CF lung disease.</description><issn>1073-449X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>FZOIL</sourceid><recordid>eNpjYeA0NDA31jUxsYzgYOAqLs4yMDA0sjA04GSw9M0vKsjIz00tKcpMVnDMS8ypLM4sVshPU3CtKMhJzCtR8CnNSy9WyMxTcK4sLgGqcctMKsoHquFhYE1LzClO5YXS3Azqbq4hzh662aU5qaVlqXnxKcUFicmp8YZGxiamZuYWlvGmhmaGBmbGpKjUJk5lfElFiTEAxytEcQ</recordid><startdate>20160301</startdate><enddate>20160301</enddate><creator>Boon, Mieke</creator><creator>Verleden, Stijn E</creator><creator>Bosch, Barbara</creator><creator>Lammertyn, Elise J</creator><creator>McDonough, John E</creator><creator>Mai, Cindy</creator><creator>Verschakelen, Johny</creator><creator>Kemner-van de Corput, Mariette</creator><creator>Tiddens, Harm A.W</creator><creator>Proesmans, Marijke</creator><creator>Vermeulen, Francois L</creator><creator>Verbeken, Erik K</creator><creator>Cooper, Joel</creator><creator>Van Raemdonck, Dirk E</creator><creator>Decramer, Marc</creator><creator>Verleden, Geert M</creator><creator>Hogg, James C</creator><creator>Dupont, Lieven J</creator><creator>Vanaudenaerde, Bart M</creator><creator>De Boeck, Kris</creator><general>AMER THORACIC SOC</general><scope>FZOIL</scope></search><sort><creationdate>20160301</creationdate><title>Morphometric Analysis of Explant Lungs in Cystic Fibrosis</title><author>Boon, Mieke ; Verleden, Stijn E ; Bosch, Barbara ; Lammertyn, Elise J ; McDonough, John E ; Mai, Cindy ; Verschakelen, Johny ; Kemner-van de Corput, Mariette ; Tiddens, Harm A.W ; Proesmans, Marijke ; Vermeulen, Francois L ; Verbeken, Erik K ; Cooper, Joel ; Van Raemdonck, Dirk E ; Decramer, Marc ; Verleden, Geert M ; Hogg, James C ; Dupont, Lieven J ; Vanaudenaerde, Bart M ; De Boeck, Kris</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-kuleuven_dspace_123456789_5161063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boon, Mieke</creatorcontrib><creatorcontrib>Verleden, Stijn E</creatorcontrib><creatorcontrib>Bosch, Barbara</creatorcontrib><creatorcontrib>Lammertyn, Elise J</creatorcontrib><creatorcontrib>McDonough, John E</creatorcontrib><creatorcontrib>Mai, Cindy</creatorcontrib><creatorcontrib>Verschakelen, Johny</creatorcontrib><creatorcontrib>Kemner-van de Corput, Mariette</creatorcontrib><creatorcontrib>Tiddens, Harm A.W</creatorcontrib><creatorcontrib>Proesmans, Marijke</creatorcontrib><creatorcontrib>Vermeulen, Francois L</creatorcontrib><creatorcontrib>Verbeken, Erik K</creatorcontrib><creatorcontrib>Cooper, Joel</creatorcontrib><creatorcontrib>Van Raemdonck, Dirk E</creatorcontrib><creatorcontrib>Decramer, Marc</creatorcontrib><creatorcontrib>Verleden, Geert M</creatorcontrib><creatorcontrib>Hogg, James C</creatorcontrib><creatorcontrib>Dupont, Lieven J</creatorcontrib><creatorcontrib>Vanaudenaerde, Bart M</creatorcontrib><creatorcontrib>De Boeck, Kris</creatorcontrib><collection>Lirias (KU Leuven Association)</collection><jtitle>AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boon, Mieke</au><au>Verleden, Stijn E</au><au>Bosch, Barbara</au><au>Lammertyn, Elise J</au><au>McDonough, John E</au><au>Mai, Cindy</au><au>Verschakelen, Johny</au><au>Kemner-van de Corput, Mariette</au><au>Tiddens, Harm A.W</au><au>Proesmans, Marijke</au><au>Vermeulen, Francois L</au><au>Verbeken, Erik K</au><au>Cooper, Joel</au><au>Van Raemdonck, Dirk E</au><au>Decramer, Marc</au><au>Verleden, Geert M</au><au>Hogg, James C</au><au>Dupont, Lieven J</au><au>Vanaudenaerde, Bart M</au><au>De Boeck, Kris</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Morphometric Analysis of Explant Lungs in Cystic Fibrosis</atitle><jtitle>AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE</jtitle><date>2016-03-01</date><risdate>2016</risdate><volume>193</volume><issue>5</issue><spage>516</spage><epage>526</epage><pages>516-526</pages><issn>1073-449X</issn><abstract>RATIONALE: After repeated cycles of lung infection and inflammation, patients with cystic fibrosis (CF) evolve to respiratory insufficiency. Although histology and imaging have provided descriptive information, a thorough morphometric analysis of end-stage CF lung disease is lacking. OBJECTIVES: To quantify the involvement of small and large airways in end-stage CF. METHODS: Multidetector computed tomography (MDCT) and micro-CT were applied to 11 air-inflated CF explanted lungs and 7 control lungs to measure, count, and describe the airway and parenchymal abnormalities in end-stage CF lungs. Selected abnormalities were further investigated with thin section histology. MEASUREMENTS AND MAIN RESULTS: On MDCT, CF explanted lungs showed an increased median (interquartile range) number (631 [511-710] vs. 344 [277-349]; P = 0.003) and size of visible airways (cumulative airway diameter 217 cm [209-250] vs. 91 cm [80-105]; P < 0.001) compared with controls. Airway obstruction was seen, starting from generation 6 and increasing to 40 to 50% of airways from generation 9 onward. Micro-CT showed that the total number of terminal bronchioles was decreased (2.9/ml [2.6-4.4] vs. 5.3/ml [4.8-5.7]; P < 0.001); 49% were obstructed, and the cross-sectional area of the open terminal bronchioles was reduced (0.093 mm(2) [0.084-0.123] vs. 0.179 mm(2) [0.140-0.196]; P < 0.001). On micro-CT, 41% of the obstructed airways reopened more distally. This remodeling was confirmed on histological analysis. Parenchymal changes were also seen, mostly in a patchy and peribronchiolar distribution. CONCLUSIONS: Extensive changes of dilatation and obstruction in nearly all airway generations were observed in end-stage CF lung disease.</abstract><pub>AMER THORACIC SOC</pub><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1073-449X |
ispartof | AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2016-03, Vol.193 (5), p.516-526 |
issn | 1073-449X |
language | eng |
recordid | cdi_kuleuven_dspace_123456789_516106 |
source | Lirias (KU Leuven Association); Journals@Ovid Complete; American Thoracic Society (ATS) Journals Online; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
title | Morphometric Analysis of Explant Lungs in Cystic Fibrosis |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T13%3A33%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-kuleuven&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Morphometric%20Analysis%20of%20Explant%20Lungs%20in%20Cystic%20Fibrosis&rft.jtitle=AMERICAN%20JOURNAL%20OF%20RESPIRATORY%20AND%20CRITICAL%20CARE%20MEDICINE&rft.au=Boon,%20Mieke&rft.date=2016-03-01&rft.volume=193&rft.issue=5&rft.spage=516&rft.epage=526&rft.pages=516-526&rft.issn=1073-449X&rft_id=info:doi/&rft_dat=%3Ckuleuven%3E123456789_516106%3C/kuleuven%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |