Genes that determine immunology and inflammation modify the basic defect of impaired ion conductance in cystic fibrosis epithelia
Background The cystic fibrosis (CF) basic defect, caused by dysfunction of the apical chloride channel CFTR in the gastrointestinal and respiratory tract epithelia, has not been employed so far to support the role of CF modifier genes. Methods Patients were selected from 101 families with a total of...
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| Veröffentlicht in: | Journal of Medical Genetics 2011-01, Vol.48 (1), p.24-31 |
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| creator | Stanke, Frauke Becker, Tim Kumar, Vinod Hedtfeld, Silke Becker, Christian Cuppens, Harry Tamm, Stephanie Yarden, Jennifer Laabs, Ulrike Siebert, Benny Fernandez, Luis Macek, Milan Radojkovic, Dragica Ballmann, Manfred Greipel, Joachim Cassiman, Jean-Jacques Wienker, Thomas F Tümmler, Burkhard |
| description | Background The cystic fibrosis (CF) basic defect, caused by dysfunction of the apical chloride channel CFTR in the gastrointestinal and respiratory tract epithelia, has not been employed so far to support the role of CF modifier genes. Methods Patients were selected from 101 families with a total of 171 F508del-CFTR homozygous CF patients to identify CF modifying genes. A candidate gene based association study of 52 genes on 16 different chromosomes with a total of 182 genetic markers was performed. Differences in haplotype and/or diplotype distribution between case and reference CF subpopulations were analysed. Results Variants at immunologically relevant genes were associated with the manifestation of the CF basic defect (0.01 |
| format | Article |
| fullrecord | <record><control><sourceid>kuleuven</sourceid><recordid>TN_cdi_kuleuven_dspace_123456789_276248</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>123456789_276248</sourcerecordid><originalsourceid>FETCH-kuleuven_dspace_123456789_2762483</originalsourceid><addsrcrecordid>eNqVjMtOwzAQRb0AqeXxD96xQJUSu03aNQL6AeyjqT2GKX5EmQkiS_4cL_iAsrq60jnnSq2bxpiN2R3sSt0wn5umtX3brdXPK2ZkLR8g2qPglCijppTmXGJ5XzRkrymHCCmBUMk6FU9hqQbqEzC5qgV0okuo2gg0YRUq50r2sxPIrvbqXVgqHOg0FSbWOFJNRII7dR0gMt7_7a16eHl-ezpuPueI8xfmwfMIDofW2O2u6_eHwfSd2e7tf8jHy8hBvsX-AsQ1X_M</addsrcrecordid><sourcetype>Institutional Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Genes that determine immunology and inflammation modify the basic defect of impaired ion conductance in cystic fibrosis epithelia</title><source>Lirias (KU Leuven Association)</source><source>BMJ Journals - NESLi2</source><creator>Stanke, Frauke ; Becker, Tim ; Kumar, Vinod ; Hedtfeld, Silke ; Becker, Christian ; Cuppens, Harry ; Tamm, Stephanie ; Yarden, Jennifer ; Laabs, Ulrike ; Siebert, Benny ; Fernandez, Luis ; Macek, Milan ; Radojkovic, Dragica ; Ballmann, Manfred ; Greipel, Joachim ; Cassiman, Jean-Jacques ; Wienker, Thomas F ; Tümmler, Burkhard</creator><creatorcontrib>Stanke, Frauke ; Becker, Tim ; Kumar, Vinod ; Hedtfeld, Silke ; Becker, Christian ; Cuppens, Harry ; Tamm, Stephanie ; Yarden, Jennifer ; Laabs, Ulrike ; Siebert, Benny ; Fernandez, Luis ; Macek, Milan ; Radojkovic, Dragica ; Ballmann, Manfred ; Greipel, Joachim ; Cassiman, Jean-Jacques ; Wienker, Thomas F ; Tümmler, Burkhard</creatorcontrib><description>Background The cystic fibrosis (CF) basic defect, caused by dysfunction of the apical chloride channel CFTR in the gastrointestinal and respiratory tract epithelia, has not been employed so far to support the role of CF modifier genes. Methods Patients were selected from 101 families with a total of 171 F508del-CFTR homozygous CF patients to identify CF modifying genes. A candidate gene based association study of 52 genes on 16 different chromosomes with a total of 182 genetic markers was performed. Differences in haplotype and/or diplotype distribution between case and reference CF subpopulations were analysed. Results Variants at immunologically relevant genes were associated with the manifestation of the CF basic defect (0.01<Praw<0.0001 at IL1B, TLR9, TNFα, CD95, STAT3 and TNFR). The intragenic background of F508del-CFTR chromosomes determined disease severity and manifestation of the basic defect (Praw=0.0009). Allele distributions comparing transmitted and non-transmitted alleles were distorted at several loci unlinked to CFTR. Conclusions The inherited capabilities of the innate and adaptive immune system determine the manifestation of the CF basic defect. Variants on F508del-CFTR chromosomes contribute to the observed patient-to-patient variability among F508del-CFTR homozygotes. A survivor effect, manifesting as a transmission disequilibrium at many loci, is consistent with the improvement of clinical care over the last decades, resulting in a depletion of risk alleles at modifier genes. Awareness of non-genetic factors such as improvement of patient care over time is crucial for the interpretation of CF modifier studies.</description><identifier>ISSN: 0022-2593</identifier><language>eng</language><publisher>BMJ Publishing Group</publisher><ispartof>Journal of Medical Genetics, 2011-01, Vol.48 (1), p.24-31</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,315,780,784,27859</link.rule.ids></links><search><creatorcontrib>Stanke, Frauke</creatorcontrib><creatorcontrib>Becker, Tim</creatorcontrib><creatorcontrib>Kumar, Vinod</creatorcontrib><creatorcontrib>Hedtfeld, Silke</creatorcontrib><creatorcontrib>Becker, Christian</creatorcontrib><creatorcontrib>Cuppens, Harry</creatorcontrib><creatorcontrib>Tamm, Stephanie</creatorcontrib><creatorcontrib>Yarden, Jennifer</creatorcontrib><creatorcontrib>Laabs, Ulrike</creatorcontrib><creatorcontrib>Siebert, Benny</creatorcontrib><creatorcontrib>Fernandez, Luis</creatorcontrib><creatorcontrib>Macek, Milan</creatorcontrib><creatorcontrib>Radojkovic, Dragica</creatorcontrib><creatorcontrib>Ballmann, Manfred</creatorcontrib><creatorcontrib>Greipel, Joachim</creatorcontrib><creatorcontrib>Cassiman, Jean-Jacques</creatorcontrib><creatorcontrib>Wienker, Thomas F</creatorcontrib><creatorcontrib>Tümmler, Burkhard</creatorcontrib><title>Genes that determine immunology and inflammation modify the basic defect of impaired ion conductance in cystic fibrosis epithelia</title><title>Journal of Medical Genetics</title><description>Background The cystic fibrosis (CF) basic defect, caused by dysfunction of the apical chloride channel CFTR in the gastrointestinal and respiratory tract epithelia, has not been employed so far to support the role of CF modifier genes. Methods Patients were selected from 101 families with a total of 171 F508del-CFTR homozygous CF patients to identify CF modifying genes. A candidate gene based association study of 52 genes on 16 different chromosomes with a total of 182 genetic markers was performed. Differences in haplotype and/or diplotype distribution between case and reference CF subpopulations were analysed. Results Variants at immunologically relevant genes were associated with the manifestation of the CF basic defect (0.01<Praw<0.0001 at IL1B, TLR9, TNFα, CD95, STAT3 and TNFR). The intragenic background of F508del-CFTR chromosomes determined disease severity and manifestation of the basic defect (Praw=0.0009). Allele distributions comparing transmitted and non-transmitted alleles were distorted at several loci unlinked to CFTR. Conclusions The inherited capabilities of the innate and adaptive immune system determine the manifestation of the CF basic defect. Variants on F508del-CFTR chromosomes contribute to the observed patient-to-patient variability among F508del-CFTR homozygotes. A survivor effect, manifesting as a transmission disequilibrium at many loci, is consistent with the improvement of clinical care over the last decades, resulting in a depletion of risk alleles at modifier genes. Awareness of non-genetic factors such as improvement of patient care over time is crucial for the interpretation of CF modifier studies.</description><issn>0022-2593</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>FZOIL</sourceid><recordid>eNqVjMtOwzAQRb0AqeXxD96xQJUSu03aNQL6AeyjqT2GKX5EmQkiS_4cL_iAsrq60jnnSq2bxpiN2R3sSt0wn5umtX3brdXPK2ZkLR8g2qPglCijppTmXGJ5XzRkrymHCCmBUMk6FU9hqQbqEzC5qgV0okuo2gg0YRUq50r2sxPIrvbqXVgqHOg0FSbWOFJNRII7dR0gMt7_7a16eHl-ezpuPueI8xfmwfMIDofW2O2u6_eHwfSd2e7tf8jHy8hBvsX-AsQ1X_M</recordid><startdate>201101</startdate><enddate>201101</enddate><creator>Stanke, Frauke</creator><creator>Becker, Tim</creator><creator>Kumar, Vinod</creator><creator>Hedtfeld, Silke</creator><creator>Becker, Christian</creator><creator>Cuppens, Harry</creator><creator>Tamm, Stephanie</creator><creator>Yarden, Jennifer</creator><creator>Laabs, Ulrike</creator><creator>Siebert, Benny</creator><creator>Fernandez, Luis</creator><creator>Macek, Milan</creator><creator>Radojkovic, Dragica</creator><creator>Ballmann, Manfred</creator><creator>Greipel, Joachim</creator><creator>Cassiman, Jean-Jacques</creator><creator>Wienker, Thomas F</creator><creator>Tümmler, Burkhard</creator><general>BMJ Publishing Group</general><scope>FZOIL</scope></search><sort><creationdate>201101</creationdate><title>Genes that determine immunology and inflammation modify the basic defect of impaired ion conductance in cystic fibrosis epithelia</title><author>Stanke, Frauke ; Becker, Tim ; Kumar, Vinod ; Hedtfeld, Silke ; Becker, Christian ; Cuppens, Harry ; Tamm, Stephanie ; Yarden, Jennifer ; Laabs, Ulrike ; Siebert, Benny ; Fernandez, Luis ; Macek, Milan ; Radojkovic, Dragica ; Ballmann, Manfred ; Greipel, Joachim ; Cassiman, Jean-Jacques ; Wienker, Thomas F ; Tümmler, Burkhard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-kuleuven_dspace_123456789_2762483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stanke, Frauke</creatorcontrib><creatorcontrib>Becker, Tim</creatorcontrib><creatorcontrib>Kumar, Vinod</creatorcontrib><creatorcontrib>Hedtfeld, Silke</creatorcontrib><creatorcontrib>Becker, Christian</creatorcontrib><creatorcontrib>Cuppens, Harry</creatorcontrib><creatorcontrib>Tamm, Stephanie</creatorcontrib><creatorcontrib>Yarden, Jennifer</creatorcontrib><creatorcontrib>Laabs, Ulrike</creatorcontrib><creatorcontrib>Siebert, Benny</creatorcontrib><creatorcontrib>Fernandez, Luis</creatorcontrib><creatorcontrib>Macek, Milan</creatorcontrib><creatorcontrib>Radojkovic, Dragica</creatorcontrib><creatorcontrib>Ballmann, Manfred</creatorcontrib><creatorcontrib>Greipel, Joachim</creatorcontrib><creatorcontrib>Cassiman, Jean-Jacques</creatorcontrib><creatorcontrib>Wienker, Thomas F</creatorcontrib><creatorcontrib>Tümmler, Burkhard</creatorcontrib><collection>Lirias (KU Leuven Association)</collection><jtitle>Journal of Medical Genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stanke, Frauke</au><au>Becker, Tim</au><au>Kumar, Vinod</au><au>Hedtfeld, Silke</au><au>Becker, Christian</au><au>Cuppens, Harry</au><au>Tamm, Stephanie</au><au>Yarden, Jennifer</au><au>Laabs, Ulrike</au><au>Siebert, Benny</au><au>Fernandez, Luis</au><au>Macek, Milan</au><au>Radojkovic, Dragica</au><au>Ballmann, Manfred</au><au>Greipel, Joachim</au><au>Cassiman, Jean-Jacques</au><au>Wienker, Thomas F</au><au>Tümmler, Burkhard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genes that determine immunology and inflammation modify the basic defect of impaired ion conductance in cystic fibrosis epithelia</atitle><jtitle>Journal of Medical Genetics</jtitle><date>2011-01</date><risdate>2011</risdate><volume>48</volume><issue>1</issue><spage>24</spage><epage>31</epage><pages>24-31</pages><issn>0022-2593</issn><abstract>Background The cystic fibrosis (CF) basic defect, caused by dysfunction of the apical chloride channel CFTR in the gastrointestinal and respiratory tract epithelia, has not been employed so far to support the role of CF modifier genes. Methods Patients were selected from 101 families with a total of 171 F508del-CFTR homozygous CF patients to identify CF modifying genes. A candidate gene based association study of 52 genes on 16 different chromosomes with a total of 182 genetic markers was performed. Differences in haplotype and/or diplotype distribution between case and reference CF subpopulations were analysed. Results Variants at immunologically relevant genes were associated with the manifestation of the CF basic defect (0.01<Praw<0.0001 at IL1B, TLR9, TNFα, CD95, STAT3 and TNFR). The intragenic background of F508del-CFTR chromosomes determined disease severity and manifestation of the basic defect (Praw=0.0009). Allele distributions comparing transmitted and non-transmitted alleles were distorted at several loci unlinked to CFTR. Conclusions The inherited capabilities of the innate and adaptive immune system determine the manifestation of the CF basic defect. Variants on F508del-CFTR chromosomes contribute to the observed patient-to-patient variability among F508del-CFTR homozygotes. A survivor effect, manifesting as a transmission disequilibrium at many loci, is consistent with the improvement of clinical care over the last decades, resulting in a depletion of risk alleles at modifier genes. Awareness of non-genetic factors such as improvement of patient care over time is crucial for the interpretation of CF modifier studies.</abstract><pub>BMJ Publishing Group</pub></addata></record> |
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| title | Genes that determine immunology and inflammation modify the basic defect of impaired ion conductance in cystic fibrosis epithelia |
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