Association of the ubiquitin specific peptidase 9X -linked and Afadin expression patterns with sexual maturation in boar testis
Closely correlated expression patterns between ubiquitin specific peptidase 9X-linked (US-P9X) and adherens junction formation factor (Afadin) in mouse testis development suggests that Usp9x regulates the deubiquitination of Af-6 (also known as Afadin, AFDN), and subsequently, the cell adhesion dyna...
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Veröffentlicht in: | Journal of animal science and technology 2021-09, Vol.63 (5), p.977-983 |
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creator | Baek, Sun-Young Lee, Seung-Hoon Kim, Youngshin Hong, Joon-Ki Cho, Eunseok Ha, Seungmin Kim, Kyungwoon Sa, Soojin Chung, Hakjae |
description | Closely correlated expression patterns between ubiquitin specific peptidase 9X-linked (US-P9X) and adherens junction formation factor (Afadin) in mouse testis development suggests that Usp9x regulates the deubiquitination of Af-6 (also known as Afadin, AFDN), and subsequently, the cell adhesion dynamics during gametogenesis. However, this relationship has not yet been tested in other domestic animals. The study was examined the temporal and spatial expression patterns of porcine USP9X and AFDN from the pre-pubertal to adult stages using real time-PCR and immunohistochemistry. Furthermore, we detected the transcripts of USP9X and AFDN in the testis of 1-, 6- and 12-months old boar, respectively. USP9X and AFDN were found to have similar expressions patterns, with basal expression after 1 month followed by a significant up-regulation from 6 months (puberty) onwards. In addition, neither the AFDN or USP9X proteins were detected in spermatogenic cells but they were expressed in the leydig cells and sertoli cells. USP9X was detected around the basal lamina during pre-puberty, and predominantly expressed in the leydig cells at puberty. Finally, in adult testis, USP9X was increased at the sertoli cell-cell interface and the sertoli cell-spermatid interface. In summary, closely correlated expression patterns between USP9X and AFDN in boar testis supports the previous findings in mice. Furthermore, the junction connections between the sertoli cells may be regulated by the ubiquitination process mediated via USP9X. |
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However, this relationship has not yet been tested in other domestic animals. The study was examined the temporal and spatial expression patterns of porcine USP9X and AFDN from the pre-pubertal to adult stages using real time-PCR and immunohistochemistry. Furthermore, we detected the transcripts of USP9X and AFDN in the testis of 1-, 6- and 12-months old boar, respectively. USP9X and AFDN were found to have similar expressions patterns, with basal expression after 1 month followed by a significant up-regulation from 6 months (puberty) onwards. In addition, neither the AFDN or USP9X proteins were detected in spermatogenic cells but they were expressed in the leydig cells and sertoli cells. USP9X was detected around the basal lamina during pre-puberty, and predominantly expressed in the leydig cells at puberty. Finally, in adult testis, USP9X was increased at the sertoli cell-cell interface and the sertoli cell-spermatid interface. In summary, closely correlated expression patterns between USP9X and AFDN in boar testis supports the previous findings in mice. Furthermore, the junction connections between the sertoli cells may be regulated by the ubiquitination process mediated via USP9X.</description><identifier>ISSN: 2672-0191</identifier><identifier>EISSN: 2093-6281</identifier><language>kor</language><publisher>한국축산학회</publisher><subject>Adherens junction formation fac-tor (AFDN) ; Boar testis ; Spermatogenesis ; Spermiogenesis ; Ubiquitin specific peptidase 9X -linked (USP9X)</subject><ispartof>Journal of animal science and technology, 2021-09, Vol.63 (5), p.977-983</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885</link.rule.ids></links><search><creatorcontrib>Baek, Sun-Young</creatorcontrib><creatorcontrib>Lee, Seung-Hoon</creatorcontrib><creatorcontrib>Kim, Youngshin</creatorcontrib><creatorcontrib>Hong, Joon-Ki</creatorcontrib><creatorcontrib>Cho, Eunseok</creatorcontrib><creatorcontrib>Ha, Seungmin</creatorcontrib><creatorcontrib>Kim, Kyungwoon</creatorcontrib><creatorcontrib>Sa, Soojin</creatorcontrib><creatorcontrib>Chung, Hakjae</creatorcontrib><title>Association of the ubiquitin specific peptidase 9X -linked and Afadin expression patterns with sexual maturation in boar testis</title><title>Journal of animal science and technology</title><addtitle>한국축산학회지</addtitle><description>Closely correlated expression patterns between ubiquitin specific peptidase 9X-linked (US-P9X) and adherens junction formation factor (Afadin) in mouse testis development suggests that Usp9x regulates the deubiquitination of Af-6 (also known as Afadin, AFDN), and subsequently, the cell adhesion dynamics during gametogenesis. However, this relationship has not yet been tested in other domestic animals. The study was examined the temporal and spatial expression patterns of porcine USP9X and AFDN from the pre-pubertal to adult stages using real time-PCR and immunohistochemistry. Furthermore, we detected the transcripts of USP9X and AFDN in the testis of 1-, 6- and 12-months old boar, respectively. USP9X and AFDN were found to have similar expressions patterns, with basal expression after 1 month followed by a significant up-regulation from 6 months (puberty) onwards. In addition, neither the AFDN or USP9X proteins were detected in spermatogenic cells but they were expressed in the leydig cells and sertoli cells. USP9X was detected around the basal lamina during pre-puberty, and predominantly expressed in the leydig cells at puberty. Finally, in adult testis, USP9X was increased at the sertoli cell-cell interface and the sertoli cell-spermatid interface. In summary, closely correlated expression patterns between USP9X and AFDN in boar testis supports the previous findings in mice. Furthermore, the junction connections between the sertoli cells may be regulated by the ubiquitination process mediated via USP9X.</description><subject>Adherens junction formation fac-tor (AFDN)</subject><subject>Boar testis</subject><subject>Spermatogenesis</subject><subject>Spermiogenesis</subject><subject>Ubiquitin specific peptidase 9X -linked (USP9X)</subject><issn>2672-0191</issn><issn>2093-6281</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>JDI</sourceid><recordid>eNo9jb1qwzAYRU1poSHNE3TR0tEgfbItaTShvwlkydDNyPohH3Fs15JpOvXVq5LS6dzhcO5VtgCqeF6BZNdpVwJyyhS7zVYhYEsLoEyqUi6y7zqEwaCOOPRk8CQeHJlb_JgxYk_C6Ax6NGR0Y0SrgyPqneQd9kdnie4tqb22SXTncXIpnSKjjtFNfSCfGA8kuPOsO3LScZ4uJ8luBz2R6ELEcJfdeN0Ft_rjMts_Pe7XL_l29_y6rrf5saQidyVT3moqvTG0qH6hLVdGeVpxS8EzScF4AMtaw1ujSs4VtbbypaoElXyZPVyyR0yvTW9D17zVmx1QYKCASyZEASJ59_9eaMYJT3r6alJLCAn8B5qfZrg</recordid><startdate>20210901</startdate><enddate>20210901</enddate><creator>Baek, Sun-Young</creator><creator>Lee, Seung-Hoon</creator><creator>Kim, Youngshin</creator><creator>Hong, Joon-Ki</creator><creator>Cho, Eunseok</creator><creator>Ha, Seungmin</creator><creator>Kim, Kyungwoon</creator><creator>Sa, Soojin</creator><creator>Chung, Hakjae</creator><general>한국축산학회</general><scope>HZB</scope><scope>Q5X</scope><scope>JDI</scope></search><sort><creationdate>20210901</creationdate><title>Association of the ubiquitin specific peptidase 9X -linked and Afadin expression patterns with sexual maturation in boar testis</title><author>Baek, Sun-Young ; Lee, Seung-Hoon ; Kim, Youngshin ; Hong, Joon-Ki ; Cho, Eunseok ; Ha, Seungmin ; Kim, Kyungwoon ; Sa, Soojin ; Chung, Hakjae</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-k507-e519fda08fcc0468fccad39c9f063d02f1802cf22d1bc3bc953390dd6f5967083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>kor</language><creationdate>2021</creationdate><topic>Adherens junction formation fac-tor (AFDN)</topic><topic>Boar testis</topic><topic>Spermatogenesis</topic><topic>Spermiogenesis</topic><topic>Ubiquitin specific peptidase 9X -linked (USP9X)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baek, Sun-Young</creatorcontrib><creatorcontrib>Lee, Seung-Hoon</creatorcontrib><creatorcontrib>Kim, Youngshin</creatorcontrib><creatorcontrib>Hong, Joon-Ki</creatorcontrib><creatorcontrib>Cho, Eunseok</creatorcontrib><creatorcontrib>Ha, Seungmin</creatorcontrib><creatorcontrib>Kim, Kyungwoon</creatorcontrib><creatorcontrib>Sa, Soojin</creatorcontrib><creatorcontrib>Chung, Hakjae</creatorcontrib><collection>KISS</collection><collection>Korean Studies Information Service System (KISS) B-Type</collection><collection>KoreaScience</collection><jtitle>Journal of animal science and technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baek, Sun-Young</au><au>Lee, Seung-Hoon</au><au>Kim, Youngshin</au><au>Hong, Joon-Ki</au><au>Cho, Eunseok</au><au>Ha, Seungmin</au><au>Kim, Kyungwoon</au><au>Sa, Soojin</au><au>Chung, Hakjae</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Association of the ubiquitin specific peptidase 9X -linked and Afadin expression patterns with sexual maturation in boar testis</atitle><jtitle>Journal of animal science and technology</jtitle><addtitle>한국축산학회지</addtitle><date>2021-09-01</date><risdate>2021</risdate><volume>63</volume><issue>5</issue><spage>977</spage><epage>983</epage><pages>977-983</pages><issn>2672-0191</issn><eissn>2093-6281</eissn><abstract>Closely correlated expression patterns between ubiquitin specific peptidase 9X-linked (US-P9X) and adherens junction formation factor (Afadin) in mouse testis development suggests that Usp9x regulates the deubiquitination of Af-6 (also known as Afadin, AFDN), and subsequently, the cell adhesion dynamics during gametogenesis. However, this relationship has not yet been tested in other domestic animals. The study was examined the temporal and spatial expression patterns of porcine USP9X and AFDN from the pre-pubertal to adult stages using real time-PCR and immunohistochemistry. Furthermore, we detected the transcripts of USP9X and AFDN in the testis of 1-, 6- and 12-months old boar, respectively. USP9X and AFDN were found to have similar expressions patterns, with basal expression after 1 month followed by a significant up-regulation from 6 months (puberty) onwards. In addition, neither the AFDN or USP9X proteins were detected in spermatogenic cells but they were expressed in the leydig cells and sertoli cells. USP9X was detected around the basal lamina during pre-puberty, and predominantly expressed in the leydig cells at puberty. Finally, in adult testis, USP9X was increased at the sertoli cell-cell interface and the sertoli cell-spermatid interface. In summary, closely correlated expression patterns between USP9X and AFDN in boar testis supports the previous findings in mice. Furthermore, the junction connections between the sertoli cells may be regulated by the ubiquitination process mediated via USP9X.</abstract><pub>한국축산학회</pub><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Adherens junction formation fac-tor (AFDN) Boar testis Spermatogenesis Spermiogenesis Ubiquitin specific peptidase 9X -linked (USP9X) |
title | Association of the ubiquitin specific peptidase 9X -linked and Afadin expression patterns with sexual maturation in boar testis |
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