Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake

Tricholoma matsutake is an ectomycorrhizal fungus, related with the host of Pinus densiflora. Most of studies on T. matsutake have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and immune activity of this strain. T. matsutake is known for its unique fragrance in Eastern A...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of microbiology and biotechnology 2020-02, Vol.30 (2), p.296-305
Hauptverfasser: Lee, Nan-Yeong, Choi, Doo-Ho, Kim, Mi-Gyeong, Jeong, Min-Ji, Kwon, Hae-Jun, Kim, Dong-Hyun, Kim, Young-Guk, Luccio, Eric di, Arioka, Manabu, Yoon, Hyeok-Jun, Kim, Jong-Guk
Format: Artikel
Sprache:kor
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 305
container_issue 2
container_start_page 296
container_title Journal of microbiology and biotechnology
container_volume 30
creator Lee, Nan-Yeong
Choi, Doo-Ho
Kim, Mi-Gyeong
Jeong, Min-Ji
Kwon, Hae-Jun
Kim, Dong-Hyun
Kim, Young-Guk
Luccio, Eric di
Arioka, Manabu
Yoon, Hyeok-Jun
Kim, Jong-Guk
description Tricholoma matsutake is an ectomycorrhizal fungus, related with the host of Pinus densiflora. Most of studies on T. matsutake have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and immune activity of this strain. T. matsutake is known for its unique fragrance in Eastern Asia. The most major component of its scent is (R)-(-)-1-octen-3-ol and is biosynthesized from the substrate linoleic acid by the sequential reaction of lipoxygenase and peroxide lyase. Here, we report for the first time the biosynthesis of (R)-(-)- 1-octen-3-ol of T. matsutake using the yeast Saccharomyces cerevisiae as a host. In this study, cDNA genes correlated with these reactions were cloned from T. matsutake, and expression studies of theses genes were carried out in the yeast Saccharomyces cerevisiae. The product of these genes expression study was carried out with Western blotting. The biosynthesis of (R)-(-)- 1-octen-3-ol of T. matsutake in recombinant Saccharomyces cerevisiae was subsequently identified with GC-MS chromatography analysis. The biosynthesis of (R)-(-)-1-octen-3-ol with S. cerevisiae represents a significant step forward.
format Article
fullrecord <record><control><sourceid>kiss_kisti</sourceid><recordid>TN_cdi_kisti_ndsl_JAKO202011563148563</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><kiss_id>3753748</kiss_id><sourcerecordid>3753748</sourcerecordid><originalsourceid>FETCH-LOGICAL-k503-b7dde2601cad4ad32dff356401c9c7003dfeb76375e649e589d825e72eb9c97b3</originalsourceid><addsrcrecordid>eNo9kM1OwzAQhCMEEqXwBFx8QWoPlvyTxM6xVNACkSqV3iPH3hDTxq7iAM2b8LhYAnGZXc3Ofoc5SyZUcImlFOw87oQKLCTLLpOrEN4JySmT-ST5vrc-jG5oIdiAfINm2zme4TmmeKMHcJhjf0DWoS1o39XWKTegV6V1q3rfjRoC0tDDpw1WAfqyQ4tKe_Sn8Q2cCoApUs6g9Wh6f4Ten6wBVI7xglbg4nMTKWjXW936g-8U6tQQPga1h-vkolGHADd_c5rsHh92yzUuN6un5aLE-4xwXAtjgOWEamVSZTgzTcOzPI1GoQUh3DRQi5yLDPK0gEwWJnYAgkFd6ELUfJrc_WL3Ngy2ciYcqufFy4YRRijNck5TGTXmbv9zoTr2tlP9WEUuF6nkP0lIbTw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake</title><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Lee, Nan-Yeong ; Choi, Doo-Ho ; Kim, Mi-Gyeong ; Jeong, Min-Ji ; Kwon, Hae-Jun ; Kim, Dong-Hyun ; Kim, Young-Guk ; Luccio, Eric di ; Arioka, Manabu ; Yoon, Hyeok-Jun ; Kim, Jong-Guk</creator><creatorcontrib>Lee, Nan-Yeong ; Choi, Doo-Ho ; Kim, Mi-Gyeong ; Jeong, Min-Ji ; Kwon, Hae-Jun ; Kim, Dong-Hyun ; Kim, Young-Guk ; Luccio, Eric di ; Arioka, Manabu ; Yoon, Hyeok-Jun ; Kim, Jong-Guk</creatorcontrib><description>Tricholoma matsutake is an ectomycorrhizal fungus, related with the host of Pinus densiflora. Most of studies on T. matsutake have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and immune activity of this strain. T. matsutake is known for its unique fragrance in Eastern Asia. The most major component of its scent is (R)-(-)-1-octen-3-ol and is biosynthesized from the substrate linoleic acid by the sequential reaction of lipoxygenase and peroxide lyase. Here, we report for the first time the biosynthesis of (R)-(-)- 1-octen-3-ol of T. matsutake using the yeast Saccharomyces cerevisiae as a host. In this study, cDNA genes correlated with these reactions were cloned from T. matsutake, and expression studies of theses genes were carried out in the yeast Saccharomyces cerevisiae. The product of these genes expression study was carried out with Western blotting. The biosynthesis of (R)-(-)- 1-octen-3-ol of T. matsutake in recombinant Saccharomyces cerevisiae was subsequently identified with GC-MS chromatography analysis. The biosynthesis of (R)-(-)-1-octen-3-ol with S. cerevisiae represents a significant step forward.</description><identifier>ISSN: 1017-7825</identifier><identifier>EISSN: 1738-8872</identifier><language>kor</language><publisher>한국미생물생명공학회</publisher><subject>(R)-(-)-1-octen-3-ol ; hydroperoxide lyase ; lipoxygenase ; Saccharomyces cerevisiae ; Tricholoma matsutake</subject><ispartof>Journal of microbiology and biotechnology, 2020-02, Vol.30 (2), p.296-305</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885</link.rule.ids></links><search><creatorcontrib>Lee, Nan-Yeong</creatorcontrib><creatorcontrib>Choi, Doo-Ho</creatorcontrib><creatorcontrib>Kim, Mi-Gyeong</creatorcontrib><creatorcontrib>Jeong, Min-Ji</creatorcontrib><creatorcontrib>Kwon, Hae-Jun</creatorcontrib><creatorcontrib>Kim, Dong-Hyun</creatorcontrib><creatorcontrib>Kim, Young-Guk</creatorcontrib><creatorcontrib>Luccio, Eric di</creatorcontrib><creatorcontrib>Arioka, Manabu</creatorcontrib><creatorcontrib>Yoon, Hyeok-Jun</creatorcontrib><creatorcontrib>Kim, Jong-Guk</creatorcontrib><title>Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake</title><title>Journal of microbiology and biotechnology</title><addtitle>Journal of Microbiology and Biotechnology</addtitle><description>Tricholoma matsutake is an ectomycorrhizal fungus, related with the host of Pinus densiflora. Most of studies on T. matsutake have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and immune activity of this strain. T. matsutake is known for its unique fragrance in Eastern Asia. The most major component of its scent is (R)-(-)-1-octen-3-ol and is biosynthesized from the substrate linoleic acid by the sequential reaction of lipoxygenase and peroxide lyase. Here, we report for the first time the biosynthesis of (R)-(-)- 1-octen-3-ol of T. matsutake using the yeast Saccharomyces cerevisiae as a host. In this study, cDNA genes correlated with these reactions were cloned from T. matsutake, and expression studies of theses genes were carried out in the yeast Saccharomyces cerevisiae. The product of these genes expression study was carried out with Western blotting. The biosynthesis of (R)-(-)- 1-octen-3-ol of T. matsutake in recombinant Saccharomyces cerevisiae was subsequently identified with GC-MS chromatography analysis. The biosynthesis of (R)-(-)-1-octen-3-ol with S. cerevisiae represents a significant step forward.</description><subject>(R)-(-)-1-octen-3-ol</subject><subject>hydroperoxide lyase</subject><subject>lipoxygenase</subject><subject>Saccharomyces cerevisiae</subject><subject>Tricholoma matsutake</subject><issn>1017-7825</issn><issn>1738-8872</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>JDI</sourceid><recordid>eNo9kM1OwzAQhCMEEqXwBFx8QWoPlvyTxM6xVNACkSqV3iPH3hDTxq7iAM2b8LhYAnGZXc3Ofoc5SyZUcImlFOw87oQKLCTLLpOrEN4JySmT-ST5vrc-jG5oIdiAfINm2zme4TmmeKMHcJhjf0DWoS1o39XWKTegV6V1q3rfjRoC0tDDpw1WAfqyQ4tKe_Sn8Q2cCoApUs6g9Wh6f4Ten6wBVI7xglbg4nMTKWjXW936g-8U6tQQPga1h-vkolGHADd_c5rsHh92yzUuN6un5aLE-4xwXAtjgOWEamVSZTgzTcOzPI1GoQUh3DRQi5yLDPK0gEwWJnYAgkFd6ELUfJrc_WL3Ngy2ciYcqufFy4YRRijNck5TGTXmbv9zoTr2tlP9WEUuF6nkP0lIbTw</recordid><startdate>20200228</startdate><enddate>20200228</enddate><creator>Lee, Nan-Yeong</creator><creator>Choi, Doo-Ho</creator><creator>Kim, Mi-Gyeong</creator><creator>Jeong, Min-Ji</creator><creator>Kwon, Hae-Jun</creator><creator>Kim, Dong-Hyun</creator><creator>Kim, Young-Guk</creator><creator>Luccio, Eric di</creator><creator>Arioka, Manabu</creator><creator>Yoon, Hyeok-Jun</creator><creator>Kim, Jong-Guk</creator><general>한국미생물생명공학회</general><scope>HZB</scope><scope>Q5X</scope><scope>JDI</scope></search><sort><creationdate>20200228</creationdate><title>Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake</title><author>Lee, Nan-Yeong ; Choi, Doo-Ho ; Kim, Mi-Gyeong ; Jeong, Min-Ji ; Kwon, Hae-Jun ; Kim, Dong-Hyun ; Kim, Young-Guk ; Luccio, Eric di ; Arioka, Manabu ; Yoon, Hyeok-Jun ; Kim, Jong-Guk</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-k503-b7dde2601cad4ad32dff356401c9c7003dfeb76375e649e589d825e72eb9c97b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>kor</language><creationdate>2020</creationdate><topic>(R)-(-)-1-octen-3-ol</topic><topic>hydroperoxide lyase</topic><topic>lipoxygenase</topic><topic>Saccharomyces cerevisiae</topic><topic>Tricholoma matsutake</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Nan-Yeong</creatorcontrib><creatorcontrib>Choi, Doo-Ho</creatorcontrib><creatorcontrib>Kim, Mi-Gyeong</creatorcontrib><creatorcontrib>Jeong, Min-Ji</creatorcontrib><creatorcontrib>Kwon, Hae-Jun</creatorcontrib><creatorcontrib>Kim, Dong-Hyun</creatorcontrib><creatorcontrib>Kim, Young-Guk</creatorcontrib><creatorcontrib>Luccio, Eric di</creatorcontrib><creatorcontrib>Arioka, Manabu</creatorcontrib><creatorcontrib>Yoon, Hyeok-Jun</creatorcontrib><creatorcontrib>Kim, Jong-Guk</creatorcontrib><collection>Korean Studies Information Service System (KISS)</collection><collection>Korean Studies Information Service System (KISS) B-Type</collection><collection>KoreaScience</collection><jtitle>Journal of microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Nan-Yeong</au><au>Choi, Doo-Ho</au><au>Kim, Mi-Gyeong</au><au>Jeong, Min-Ji</au><au>Kwon, Hae-Jun</au><au>Kim, Dong-Hyun</au><au>Kim, Young-Guk</au><au>Luccio, Eric di</au><au>Arioka, Manabu</au><au>Yoon, Hyeok-Jun</au><au>Kim, Jong-Guk</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake</atitle><jtitle>Journal of microbiology and biotechnology</jtitle><addtitle>Journal of Microbiology and Biotechnology</addtitle><date>2020-02-28</date><risdate>2020</risdate><volume>30</volume><issue>2</issue><spage>296</spage><epage>305</epage><pages>296-305</pages><issn>1017-7825</issn><eissn>1738-8872</eissn><abstract>Tricholoma matsutake is an ectomycorrhizal fungus, related with the host of Pinus densiflora. Most of studies on T. matsutake have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and immune activity of this strain. T. matsutake is known for its unique fragrance in Eastern Asia. The most major component of its scent is (R)-(-)-1-octen-3-ol and is biosynthesized from the substrate linoleic acid by the sequential reaction of lipoxygenase and peroxide lyase. Here, we report for the first time the biosynthesis of (R)-(-)- 1-octen-3-ol of T. matsutake using the yeast Saccharomyces cerevisiae as a host. In this study, cDNA genes correlated with these reactions were cloned from T. matsutake, and expression studies of theses genes were carried out in the yeast Saccharomyces cerevisiae. The product of these genes expression study was carried out with Western blotting. The biosynthesis of (R)-(-)- 1-octen-3-ol of T. matsutake in recombinant Saccharomyces cerevisiae was subsequently identified with GC-MS chromatography analysis. The biosynthesis of (R)-(-)-1-octen-3-ol with S. cerevisiae represents a significant step forward.</abstract><pub>한국미생물생명공학회</pub><tpages>10</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1017-7825
ispartof Journal of microbiology and biotechnology, 2020-02, Vol.30 (2), p.296-305
issn 1017-7825
1738-8872
language kor
recordid cdi_kisti_ndsl_JAKO202011563148563
source EZB-FREE-00999 freely available EZB journals; PubMed Central
subjects (R)-(-)-1-octen-3-ol
hydroperoxide lyase
lipoxygenase
Saccharomyces cerevisiae
Tricholoma matsutake
title Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T00%3A12%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-kiss_kisti&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Biosynthesis%20of%20(R)-(-)-1-Octen-3-ol%20in%20Recombinant%20Saccharomyces%20cerevisiae%20with%20Lipoxygenase-1%20and%20Hydroperoxide%20Lyase%20Genes%20from%20Tricholoma%20matsutake&rft.jtitle=Journal%20of%20microbiology%20and%20biotechnology&rft.au=Lee,%20Nan-Yeong&rft.date=2020-02-28&rft.volume=30&rft.issue=2&rft.spage=296&rft.epage=305&rft.pages=296-305&rft.issn=1017-7825&rft.eissn=1738-8872&rft_id=info:doi/&rft_dat=%3Ckiss_kisti%3E3753748%3C/kiss_kisti%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_kiss_id=3753748&rfr_iscdi=true