Altering UDP-Glucose Donor Substrate Specificity of Bacillus licheniformis Glycosyltransferase towards TDP-Glucose

The specificity of a Bacillus licheniformis uridine diphosphate (UDP) glycosyltransferase, YjiC, was increased towards thymidine diphosphate (TDP)-sugar by site-directed mutagenesis. The Arg-282 of YjiC was identified and investigated by substituting with Trp. Conversion rate and kinetic parameters...

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Veröffentlicht in:	Journal of microbiology and biotechnology 2019-02, Vol.29 (2), p.268-273
Hauptverfasser:	Cho, Kye Woon, Kim, Tae-Su, Le, Tuoi Thi, Nguyen, Hue Thi, Oh, So Yeong, Pandey, Ramesh Prasad, Sohng, Jae Kyung
Format:	Artikel
Sprache:	kor
Schlagworte:	enzyme kinetics Glycosyltransferase mutation protein engineering TDP-glucose substrate specificity
Online-Zugang:	Volltext
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