Generation of an Infectious Clone of a New Korean Isolate of Apple chlorotic leaf spot virus Driven by Dual 35S and T7 Promoters in a Versatile Binary Vector
The full-length sequence of a new isolate of Apple chlorotic leaf spot virus (ACLSV) from Korea was divergent, but most closely related to the Japanese isolate A4, at 84% nucleotide identity. The full-length cDNA of the Korean isolate of ACLSV was cloned into a binary vector downstream of the bacter...
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| Veröffentlicht in: | The plant pathology journal 2017-12, Vol.33 (6), p.608-613 |
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| container_end_page | 613 |
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| container_title | The plant pathology journal |
| container_volume | 33 |
| creator | Kim, Ik-Hyun Han, Jae-Yeong Cho, In-Sook Ju, HyeKyoung Moon, Jae Sun Seo, Eun-Young Kim, Hong Gi Hammond, John Lim, Hyoun-Sub |
| description | The full-length sequence of a new isolate of Apple chlorotic leaf spot virus (ACLSV) from Korea was divergent, but most closely related to the Japanese isolate A4, at 84% nucleotide identity. The full-length cDNA of the Korean isolate of ACLSV was cloned into a binary vector downstream of the bacteriophage T7 RNA promoter and the Cauliflower mosaic virus 35S promoter. Chenopodium quinoa was successfully infected using in vitro transcripts synthesized using the T7 promoter, detected at 20 days post inoculation (dpi), but did not produce obvious symptoms. Nicotiana occidentalis and C. quinoa were inoculated through agroinfiltration. At 32 dpi the infection rate was evaluated; no C. quinoa plants were infected by agroinfiltration, but infection of N. occidentalis was obtained. |
| format | Article |
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| ispartof | The plant pathology journal, 2017-12, Vol.33 (6), p.608-613 |
| issn | 1598-2254 2093-9280 |
| language | kor |
| recordid | cdi_kisti_ndsl_JAKO201708160568495 |
| source | DOAJ Directory of Open Access Journals; PubMed Central |
| title | Generation of an Infectious Clone of a New Korean Isolate of Apple chlorotic leaf spot virus Driven by Dual 35S and T7 Promoters in a Versatile Binary Vector |
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