Pseudomonas aeruginosa exotoxin A(PEA)가 사람혀 편평암종세포에서 나타나는 세포자멸사 작용

Pseudomonas aeruginosa exotoxin A(PEA)가 사람혀 편평암종세포에서 나타나는 세포자멸사 작용

Objectives : The purpose of the study is to examine the apoptotic effects of Pseudomonas aeruginosa exotoxin A(PEA) in squamous cell carcinoma(SCC) 25 cells. Methods : Cell growth reduction and apoptosis induced by PEA were confirmed by WST-1 assay, Hoechst 33258 staining, flow cytometry analysis, a...

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Veröffentlicht in:Han'guk Ch'iwisaeng Hakhoe chi 2014-08, Vol.14 (4), p.601-608
Hauptverfasser: 최별보라, Byul Bora Choi, 김규천, Gyoo Cheon Kim
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apoptosis
Pseudomonas aeruginosa exotoxin A
squamous cell carcinoma
녹농균 외독소 A
세포자멸사
편평상피암
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container_title Han'guk Ch'iwisaeng Hakhoe chi
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creator 최별보라
Byul Bora Choi
김규천
Gyoo Cheon Kim
description Objectives : The purpose of the study is to examine the apoptotic effects of Pseudomonas aeruginosa exotoxin A(PEA) in squamous cell carcinoma(SCC) 25 cells. Methods : Cell growth reduction and apoptosis induced by PEA were confirmed by WST-1 assay, Hoechst 33258 staining, flow cytometry analysis, and Western blot assay. Results : The PEA treatment decreased the cell viability in a dose and time dependent manner: control; 100±0e(p
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Methods : Cell growth reduction and apoptosis induced by PEA were confirmed by WST-1 assay, Hoechst 33258 staining, flow cytometry analysis, and Western blot assay. Results : The PEA treatment decreased the cell viability in a dose and time dependent manner: control; 100±0e(p<0.01), 0.1875 nM; 87±4.36d(p<0.01), 0.375 nM; 82±0.58d(p<0.01), 0.75 nM; 72±1.67c(p<0.01), 1.5 nM; 51±1.53bc(p<0.01), 7.5 nM; 31±1.20ab(p<0.01), 15 nM; 26±0.67a(p<0.01), control; 100±0a(p<0.05), 24 h; 51±1.53b(p<0.05), 48 h; 16±0.5c(p<0.05), 72 h; 12±1.67d%(p<0.05). The PEA was observed on SCC 25 cells with the half maximal inhibitory concentration(IC50) value of 1.5 nM at 24 hours. The PEA treated SCC 25 cells demonstrated several types of apoptotic indications, such as nuclear condensation, the increase of sub G1, and the cleavage of PARP-1 and DFF 45. Conclusions : PEA showed anti-cancer activity against SCC 25 cells via apoptosis. PEA may potentially contribute to human oral cancer treatment.]]></description><identifier>ISSN: 2287-1705</identifier><identifier>EISSN: 2288-2294</identifier><language>kor</language><publisher>한국치위생학회</publisher><subject>apoptosis ; Pseudomonas aeruginosa exotoxin A ; squamous cell carcinoma ; 녹농균 외독소 A ; 세포자멸사 ; 편평상피암</subject><ispartof>Han'guk Ch'iwisaeng Hakhoe chi, 2014-08, Vol.14 (4), p.601-608</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881</link.rule.ids></links><search><creatorcontrib>최별보라</creatorcontrib><creatorcontrib>Byul Bora Choi</creatorcontrib><creatorcontrib>김규천</creatorcontrib><creatorcontrib>Gyoo Cheon Kim</creatorcontrib><title>Pseudomonas aeruginosa exotoxin A(PEA)가 사람혀 편평암종세포에서 나타나는 세포자멸사 작용</title><title>Han'guk Ch'iwisaeng Hakhoe chi</title><addtitle>한국치위생학회지</addtitle><description><![CDATA[Objectives : The purpose of the study is to examine the apoptotic effects of Pseudomonas aeruginosa exotoxin A(PEA) in squamous cell carcinoma(SCC) 25 cells. Methods : Cell growth reduction and apoptosis induced by PEA were confirmed by WST-1 assay, Hoechst 33258 staining, flow cytometry analysis, and Western blot assay. Results : The PEA treatment decreased the cell viability in a dose and time dependent manner: control; 100±0e(p<0.01), 0.1875 nM; 87±4.36d(p<0.01), 0.375 nM; 82±0.58d(p<0.01), 0.75 nM; 72±1.67c(p<0.01), 1.5 nM; 51±1.53bc(p<0.01), 7.5 nM; 31±1.20ab(p<0.01), 15 nM; 26±0.67a(p<0.01), control; 100±0a(p<0.05), 24 h; 51±1.53b(p<0.05), 48 h; 16±0.5c(p<0.05), 72 h; 12±1.67d%(p<0.05). The PEA was observed on SCC 25 cells with the half maximal inhibitory concentration(IC50) value of 1.5 nM at 24 hours. The PEA treated SCC 25 cells demonstrated several types of apoptotic indications, such as nuclear condensation, the increase of sub G1, and the cleavage of PARP-1 and DFF 45. Conclusions : PEA showed anti-cancer activity against SCC 25 cells via apoptosis. PEA may potentially contribute to human oral cancer treatment.]]></description><subject>apoptosis</subject><subject>Pseudomonas aeruginosa exotoxin A</subject><subject>squamous cell carcinoma</subject><subject>녹농균 외독소 A</subject><subject>세포자멸사</subject><subject>편평상피암</subject><issn>2287-1705</issn><issn>2288-2294</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>JDI</sourceid><recordid>eNo9Tz9Lw0AcDaJgqf0ELrcIOgR-d5dLLmMo9W-hHbqHS5NIaJuUXgt1K7UOimiFikUkg1uhg2M_k7l8B4MWl_cevD_wdrQSIZzrhNjG7q-2dGwB29cqUkYeMLAowZiUtH5TBiM_6SWxkEgEg9F1FCdSoGCcDJNxFCPnuFlzTr6_JkhN11n6mC8nKH_a5M_36nWhPu_UrNBr9TZXsw-UTZf57aTA7GGBtk46z1abootU-qLeVwfaXii6Mqhsuay1Tmut6rleb5xdVJ263mFAdM9kQLGwWWAzA9ptDphhTxjgcxK2DSyKM4KZITdtEUAQCo4BDMo97AubC0bL2tHfbCeSw8iNfdl1L52rBgFsUMCmDYyYFilyh_856fYHUU8MblxKTKAGpz9VaXTn</recordid><startdate>20140830</startdate><enddate>20140830</enddate><creator>최별보라</creator><creator>Byul Bora Choi</creator><creator>김규천</creator><creator>Gyoo Cheon Kim</creator><general>한국치위생학회</general><scope>HZB</scope><scope>Q5X</scope><scope>JDI</scope></search><sort><creationdate>20140830</creationdate><title>Pseudomonas aeruginosa exotoxin A(PEA)가 사람혀 편평암종세포에서 나타나는 세포자멸사 작용</title><author>최별보라 ; Byul Bora Choi ; 김규천 ; Gyoo Cheon Kim</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-k502-b65031a95e9540cc80151ba40d82fc41a705a56f869ae0efa8100438b1da98a53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>kor</language><creationdate>2014</creationdate><topic>apoptosis</topic><topic>Pseudomonas aeruginosa exotoxin A</topic><topic>squamous cell carcinoma</topic><topic>녹농균 외독소 A</topic><topic>세포자멸사</topic><topic>편평상피암</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>최별보라</creatorcontrib><creatorcontrib>Byul Bora Choi</creatorcontrib><creatorcontrib>김규천</creatorcontrib><creatorcontrib>Gyoo Cheon Kim</creatorcontrib><collection>Korean Studies Information Service System (KISS)</collection><collection>Korean Studies Information Service System (KISS) B-Type</collection><collection>KoreaScience</collection><jtitle>Han'guk Ch'iwisaeng Hakhoe chi</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>최별보라</au><au>Byul Bora Choi</au><au>김규천</au><au>Gyoo Cheon Kim</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pseudomonas aeruginosa exotoxin A(PEA)가 사람혀 편평암종세포에서 나타나는 세포자멸사 작용</atitle><jtitle>Han'guk Ch'iwisaeng Hakhoe chi</jtitle><addtitle>한국치위생학회지</addtitle><date>2014-08-30</date><risdate>2014</risdate><volume>14</volume><issue>4</issue><spage>601</spage><epage>608</epage><pages>601-608</pages><issn>2287-1705</issn><eissn>2288-2294</eissn><abstract><![CDATA[Objectives : The purpose of the study is to examine the apoptotic effects of Pseudomonas aeruginosa exotoxin A(PEA) in squamous cell carcinoma(SCC) 25 cells. Methods : Cell growth reduction and apoptosis induced by PEA were confirmed by WST-1 assay, Hoechst 33258 staining, flow cytometry analysis, and Western blot assay. Results : The PEA treatment decreased the cell viability in a dose and time dependent manner: control; 100±0e(p<0.01), 0.1875 nM; 87±4.36d(p<0.01), 0.375 nM; 82±0.58d(p<0.01), 0.75 nM; 72±1.67c(p<0.01), 1.5 nM; 51±1.53bc(p<0.01), 7.5 nM; 31±1.20ab(p<0.01), 15 nM; 26±0.67a(p<0.01), control; 100±0a(p<0.05), 24 h; 51±1.53b(p<0.05), 48 h; 16±0.5c(p<0.05), 72 h; 12±1.67d%(p<0.05). The PEA was observed on SCC 25 cells with the half maximal inhibitory concentration(IC50) value of 1.5 nM at 24 hours. The PEA treated SCC 25 cells demonstrated several types of apoptotic indications, such as nuclear condensation, the increase of sub G1, and the cleavage of PARP-1 and DFF 45. Conclusions : PEA showed anti-cancer activity against SCC 25 cells via apoptosis. PEA may potentially contribute to human oral cancer treatment.]]></abstract><pub>한국치위생학회</pub><tpages>7</tpages><oa>free_for_read</oa></addata></record>
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subjects apoptosis
Pseudomonas aeruginosa exotoxin A
squamous cell carcinoma
녹농균 외독소 A
세포자멸사
편평상피암
title Pseudomonas aeruginosa exotoxin A(PEA)가 사람혀 편평암종세포에서 나타나는 세포자멸사 작용
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