Reconstruction of radial bone defect using gelatin sponge and a BMP-2 combination graft
Many bioactive molecules like recombinant human bone morphogenetic protein 2 (rhBMP-2) have been developed for mineralized bone grafts, for which proper scaffolds are necessary to successfully apply the bioactive molecules. In this study, we tested the osteogenic efficacy of rhBMP-2 produced in-hous...
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| Veröffentlicht in: | BMB reports 2013-06, Vol.46 (6), p.328-333 |
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| creator | Kim, Seong-Gon Jeong, Jae-Hwan Che, Xiangguo Park, Yong-Tae Lee, Sang-Woon Jung, Eun-Sun Choe, Senyon Choi, Je-Yong |
| description | Many bioactive molecules like recombinant human bone morphogenetic protein 2 (rhBMP-2) have been developed for mineralized bone grafts, for which proper scaffolds are necessary to successfully apply the bioactive molecules. In this study, we tested the osteogenic efficacy of rhBMP-2 produced in-house in combination with gelatin sponge as the scaffold carrier in a rabbit radial defect model. The efficacy of the rhBMP-2 was determined by alkaline phosphatase activity assay of C2C12 cells. Two groups of ten rabbits each were treated with rhBMP-2/gelatin sponge, or gelatin sponge only. At 4 weeks, rhBMP-2/gelatin sponge grafts showed more bone regeneration than gelatin sponge grafts, as determined by X-ray radiography, micro-computed tomography, and histological analyses. At 8 weeks, rhBMP-2/gelatin sponge grafts exerted much stronger osteogenic effects. The study demonstrates the improved osteogenic efficacy of the rhBMP-2/gelatin sponge grafts in a rabbit radial bone defect model acting as a bone-inductive material. |
| format | Article |
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| ispartof | BMB reports, 2013-06, Vol.46 (6), p.328-333 |
| issn | 1976-6696 1976-670X |
| language | kor |
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| source | DOAJ Directory of Open Access Journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | BMP-2 Bone defect Bone graft Gelatin sponge Osteogenic activity |
| title | Reconstruction of radial bone defect using gelatin sponge and a BMP-2 combination graft |
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