Visualization of Candidate Division OP3 Cocci in Limonene-Degrading Methanogenic Cultures

Visualization of Candidate Division OP3 Cocci in Limonene-Degrading Methanogenic Cultures

Members of candidate division OP3 were detected in 16S rRNA gene clone libraries from methanogenic enrichment cultures that utilized limonene as a carbon and energy source. We developed probes for the visualization of OP3 cells. In situ hybridization experiments with newly designed OP3-specific prob...

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Veröffentlicht in:Journal of microbiology and biotechnology 2012-04, Vol.22 (4), p.457-461
Hauptverfasser: Rotaru, Amelia-Elena, Schauer, Regina, Probian, Christina, Mussmann, Marc, Harder, Jens
Format: Artikel
Sprache:kor
Schlagworte:
Candidate division OP3
hydrocarbon degradation
Limonene
methanogenesis
monoterpene
syntrophy
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Zusammenfassung:Members of candidate division OP3 were detected in 16S rRNA gene clone libraries from methanogenic enrichment cultures that utilized limonene as a carbon and energy source. We developed probes for the visualization of OP3 cells. In situ hybridization experiments with newly designed OP3-specific probes [OP3-565 and Eub-338(VI)] revealed abundant small OP3 cocci attached to larger cells. Syntrophic Deltaproteobacteria, OP3 cells, and methanogens affiliating with Methanoculleus and Methanosaeta formed the limonenedegrading community.
ISSN:1017-7825
1738-8872