Effect of Feed Types on Ochratoxin A Disappearance in Goat Rumen Fluid
This study was conducted to investigate the effect of feed types on Ochratoxin A (OTA) degradation by Korean native goats. Rumen fluid from canulated goats fed whole roughage or 50% roughage served as a source of micro-organisms. Experiments were undertaken i) to investigate OTA degradation ability...
Gespeichert in:
Veröffentlicht in: | Asian-australasian journal of animal sciences 2011, Vol.24 (2), p.198-205 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | kor |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 205 |
---|---|
container_issue | 2 |
container_start_page | 198 |
container_title | Asian-australasian journal of animal sciences |
container_volume | 24 |
creator | Upadhaya, Santi Devi Yang, Liu Seo, Ja-Kyeom Kim, Myung-Hoo Lee, Chang-Kyu Lee, Chan-Ho Ha, Jong-K |
description | This study was conducted to investigate the effect of feed types on Ochratoxin A (OTA) degradation by Korean native goats. Rumen fluid from canulated goats fed whole roughage or 50% roughage served as a source of micro-organisms. Experiments were undertaken i) to investigate OTA degradation ability in a $2{\times}4$ factorial arrangement with different feed types (100% roughage vs. 50% roughage) and rumen fluid fractions (whole rumen fluid, cells, autoclaved rumen fluid and supernatant) supplemented with OTA ii) to evaluate OTA degradation by the rumen fluid of goats fed two different diets at different time points (0, 3, 6, 9 and 12 h) of feeding iii) to isolate potential rumen microorganisms and iv) to identify elements responsible for OTA degradation. Rumen fluid from goats fed 100% roughage had higher (p |
format | Article |
fullrecord | <record><control><sourceid>kisti</sourceid><recordid>TN_cdi_kisti_ndsl_JAKO201118861578620</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>JAKO201118861578620</sourcerecordid><originalsourceid>FETCH-kisti_ndsl_JAKO2011188615786203</originalsourceid><addsrcrecordid>eNqNjkELgjAYhkcUJOV_-C4dhU3ZpkcpV9QhCO-ydKORbeIm1L_PQz-g0_vw8BzeBYpIwVlCKeHLmTEhSZoxvkax9-aOKeZpQRiLkKi0Vm0Ap0Eo1UH9GZQHZ-HaPkYZ3NtYKOFgvBwGJUdpWwWzOjoZ4Da9lAXRT6bbopWWvVfxbzdoJ6p6f0qexgfT2M73zbm8XNP5CclzRijPWYqzf7svKpA6HA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Effect of Feed Types on Ochratoxin A Disappearance in Goat Rumen Fluid</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Upadhaya, Santi Devi ; Yang, Liu ; Seo, Ja-Kyeom ; Kim, Myung-Hoo ; Lee, Chang-Kyu ; Lee, Chan-Ho ; Ha, Jong-K</creator><creatorcontrib>Upadhaya, Santi Devi ; Yang, Liu ; Seo, Ja-Kyeom ; Kim, Myung-Hoo ; Lee, Chang-Kyu ; Lee, Chan-Ho ; Ha, Jong-K</creatorcontrib><description>This study was conducted to investigate the effect of feed types on Ochratoxin A (OTA) degradation by Korean native goats. Rumen fluid from canulated goats fed whole roughage or 50% roughage served as a source of micro-organisms. Experiments were undertaken i) to investigate OTA degradation ability in a $2{\times}4$ factorial arrangement with different feed types (100% roughage vs. 50% roughage) and rumen fluid fractions (whole rumen fluid, cells, autoclaved rumen fluid and supernatant) supplemented with OTA ii) to evaluate OTA degradation by the rumen fluid of goats fed two different diets at different time points (0, 3, 6, 9 and 12 h) of feeding iii) to isolate potential rumen microorganisms and iv) to identify elements responsible for OTA degradation. Rumen fluid from goats fed 100% roughage had higher (p<0.05) OTA degradability than 50% roughage diets. OTA degradation based on rumen fluid collection times showed that rumen fluid at 0 h showed significantly higher (p<0.05) degradability. Carboxypeptidase A (CPA) enzyme has been reported to be responsible for OTA degradation. Thus, using real time PCR, primers designed to target the CPA gene from Bacillus licheniformis could be amplified using genomic DNA from rumen fluid of goats and sequenced, thus enabling evaluation of the Bacillus population under different feeding condition and times. Our findings showed that the Bacillus population was significantly higher (p<0.05) before feeding (0 h) in animals which were fed a whole roughage diet, giving indirect evidence of OTA degradation being influenced by Bacillus sps. Thus, it can be concluded that OTA degradability is influenced by feed, feeding time and Bacillus licheniformis population.</description><identifier>ISSN: 1011-2367</identifier><identifier>EISSN: 1976-5517</identifier><language>kor</language><ispartof>Asian-australasian journal of animal sciences, 2011, Vol.24 (2), p.198-205</ispartof><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,4010</link.rule.ids></links><search><creatorcontrib>Upadhaya, Santi Devi</creatorcontrib><creatorcontrib>Yang, Liu</creatorcontrib><creatorcontrib>Seo, Ja-Kyeom</creatorcontrib><creatorcontrib>Kim, Myung-Hoo</creatorcontrib><creatorcontrib>Lee, Chang-Kyu</creatorcontrib><creatorcontrib>Lee, Chan-Ho</creatorcontrib><creatorcontrib>Ha, Jong-K</creatorcontrib><title>Effect of Feed Types on Ochratoxin A Disappearance in Goat Rumen Fluid</title><title>Asian-australasian journal of animal sciences</title><addtitle>Asian-Australasian journal of animal sciences</addtitle><description>This study was conducted to investigate the effect of feed types on Ochratoxin A (OTA) degradation by Korean native goats. Rumen fluid from canulated goats fed whole roughage or 50% roughage served as a source of micro-organisms. Experiments were undertaken i) to investigate OTA degradation ability in a $2{\times}4$ factorial arrangement with different feed types (100% roughage vs. 50% roughage) and rumen fluid fractions (whole rumen fluid, cells, autoclaved rumen fluid and supernatant) supplemented with OTA ii) to evaluate OTA degradation by the rumen fluid of goats fed two different diets at different time points (0, 3, 6, 9 and 12 h) of feeding iii) to isolate potential rumen microorganisms and iv) to identify elements responsible for OTA degradation. Rumen fluid from goats fed 100% roughage had higher (p<0.05) OTA degradability than 50% roughage diets. OTA degradation based on rumen fluid collection times showed that rumen fluid at 0 h showed significantly higher (p<0.05) degradability. Carboxypeptidase A (CPA) enzyme has been reported to be responsible for OTA degradation. Thus, using real time PCR, primers designed to target the CPA gene from Bacillus licheniformis could be amplified using genomic DNA from rumen fluid of goats and sequenced, thus enabling evaluation of the Bacillus population under different feeding condition and times. Our findings showed that the Bacillus population was significantly higher (p<0.05) before feeding (0 h) in animals which were fed a whole roughage diet, giving indirect evidence of OTA degradation being influenced by Bacillus sps. Thus, it can be concluded that OTA degradability is influenced by feed, feeding time and Bacillus licheniformis population.</description><issn>1011-2367</issn><issn>1976-5517</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>JDI</sourceid><recordid>eNqNjkELgjAYhkcUJOV_-C4dhU3ZpkcpV9QhCO-ydKORbeIm1L_PQz-g0_vw8BzeBYpIwVlCKeHLmTEhSZoxvkax9-aOKeZpQRiLkKi0Vm0Ap0Eo1UH9GZQHZ-HaPkYZ3NtYKOFgvBwGJUdpWwWzOjoZ4Da9lAXRT6bbopWWvVfxbzdoJ6p6f0qexgfT2M73zbm8XNP5CclzRijPWYqzf7svKpA6HA</recordid><startdate>2011</startdate><enddate>2011</enddate><creator>Upadhaya, Santi Devi</creator><creator>Yang, Liu</creator><creator>Seo, Ja-Kyeom</creator><creator>Kim, Myung-Hoo</creator><creator>Lee, Chang-Kyu</creator><creator>Lee, Chan-Ho</creator><creator>Ha, Jong-K</creator><scope>JDI</scope></search><sort><creationdate>2011</creationdate><title>Effect of Feed Types on Ochratoxin A Disappearance in Goat Rumen Fluid</title><author>Upadhaya, Santi Devi ; Yang, Liu ; Seo, Ja-Kyeom ; Kim, Myung-Hoo ; Lee, Chang-Kyu ; Lee, Chan-Ho ; Ha, Jong-K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-kisti_ndsl_JAKO2011188615786203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>kor</language><creationdate>2011</creationdate><toplevel>online_resources</toplevel><creatorcontrib>Upadhaya, Santi Devi</creatorcontrib><creatorcontrib>Yang, Liu</creatorcontrib><creatorcontrib>Seo, Ja-Kyeom</creatorcontrib><creatorcontrib>Kim, Myung-Hoo</creatorcontrib><creatorcontrib>Lee, Chang-Kyu</creatorcontrib><creatorcontrib>Lee, Chan-Ho</creatorcontrib><creatorcontrib>Ha, Jong-K</creatorcontrib><collection>KoreaScience</collection><jtitle>Asian-australasian journal of animal sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Upadhaya, Santi Devi</au><au>Yang, Liu</au><au>Seo, Ja-Kyeom</au><au>Kim, Myung-Hoo</au><au>Lee, Chang-Kyu</au><au>Lee, Chan-Ho</au><au>Ha, Jong-K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Feed Types on Ochratoxin A Disappearance in Goat Rumen Fluid</atitle><jtitle>Asian-australasian journal of animal sciences</jtitle><addtitle>Asian-Australasian journal of animal sciences</addtitle><date>2011</date><risdate>2011</risdate><volume>24</volume><issue>2</issue><spage>198</spage><epage>205</epage><pages>198-205</pages><issn>1011-2367</issn><eissn>1976-5517</eissn><abstract>This study was conducted to investigate the effect of feed types on Ochratoxin A (OTA) degradation by Korean native goats. Rumen fluid from canulated goats fed whole roughage or 50% roughage served as a source of micro-organisms. Experiments were undertaken i) to investigate OTA degradation ability in a $2{\times}4$ factorial arrangement with different feed types (100% roughage vs. 50% roughage) and rumen fluid fractions (whole rumen fluid, cells, autoclaved rumen fluid and supernatant) supplemented with OTA ii) to evaluate OTA degradation by the rumen fluid of goats fed two different diets at different time points (0, 3, 6, 9 and 12 h) of feeding iii) to isolate potential rumen microorganisms and iv) to identify elements responsible for OTA degradation. Rumen fluid from goats fed 100% roughage had higher (p<0.05) OTA degradability than 50% roughage diets. OTA degradation based on rumen fluid collection times showed that rumen fluid at 0 h showed significantly higher (p<0.05) degradability. Carboxypeptidase A (CPA) enzyme has been reported to be responsible for OTA degradation. Thus, using real time PCR, primers designed to target the CPA gene from Bacillus licheniformis could be amplified using genomic DNA from rumen fluid of goats and sequenced, thus enabling evaluation of the Bacillus population under different feeding condition and times. Our findings showed that the Bacillus population was significantly higher (p<0.05) before feeding (0 h) in animals which were fed a whole roughage diet, giving indirect evidence of OTA degradation being influenced by Bacillus sps. Thus, it can be concluded that OTA degradability is influenced by feed, feeding time and Bacillus licheniformis population.</abstract><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1011-2367 |
ispartof | Asian-australasian journal of animal sciences, 2011, Vol.24 (2), p.198-205 |
issn | 1011-2367 1976-5517 |
language | kor |
recordid | cdi_kisti_ndsl_JAKO201118861578620 |
source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
title | Effect of Feed Types on Ochratoxin A Disappearance in Goat Rumen Fluid |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T00%3A22%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-kisti&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20Feed%20Types%20on%20Ochratoxin%20A%20Disappearance%20in%20Goat%20Rumen%20Fluid&rft.jtitle=Asian-australasian%20journal%20of%20animal%20sciences&rft.au=Upadhaya,%20Santi%20Devi&rft.date=2011&rft.volume=24&rft.issue=2&rft.spage=198&rft.epage=205&rft.pages=198-205&rft.issn=1011-2367&rft.eissn=1976-5517&rft_id=info:doi/&rft_dat=%3Ckisti%3EJAKO201118861578620%3C/kisti%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |