농산물 중 Bifenazate의 분석법 개선 및 모니터링

The analytical method for bifenazate was developed using a HPLC (UVD). Also, analytical condition of LC/MS was set up for bifenazate. We validated the method for the precision and the reproducibility. The correlation coefficient of bifenazate ranged from 0.05 to 2.5 mg/kg was 1.0. Limit of quantitation (LOQ) was 0.01 mg/kg. To measure recoveries from agricultural products such as foxtail millet (cereal grains), kidney bean (beans), orange (fruits), perilla leaves (vegetables) and oak mushroom (mushrooms), bifenazate was spiked. Mean recoveries of bifenazate for each sample were 82.7~104.1% at the level of 0.1 mg/kg and 73.1~104.3% at the level of 0.5 mg/kg. The relative standard deviations (n=3) were 0.2~9.7%. Pesticide residues for bifenazate were investigated in 16 commodities (rice, foxtail millet, buckwheat, kidney bean, peanut, sesame, orange, grapefruit, kiwifruit, spinach, perilla leaves, leek, garlic stem, garlic, ginger and oak mushroom) collected from 22 provinces in 2009. Bifenazate was analyzed using analytical method by HPLC from 304 samples, and residue was not detected. 농산물 중 bifenazate의 잔류 실태를 조사하기 위하여 HPLC를 사용한 분석법을 개선하였고, LC/MS 분석법을 확립하였다. 개선된 분석법에 대한 직선성과 재현성에 대한 검증을 수행한 결과 bifenazate 0.05~2.5 mg/kg 범위에서 상관계수는 1.0이었고, 정량한계(LOQ)는 0.01 mg/kg이었다. 회수율은 현미(곡류), 강낭콩(콩류), 오렌지(과실류), 들깻잎(채소류), 표고버섯(버섯류)에 대해 0.1 mg/kg 수준에서 82.7~104.1%였고, 0.5 mg/kg에서는 73.1~104.3%였다. 또한 회수율에 대한 상대표준편차(n=3)는 0.2~9.7%였다. 전국 22개 지역에서 수거한 농산물 16품목(쌀, 조, 메밀, 강낭콩, 땅콩, 참깨, 오렌지, 자몽, 키위, 시금치, 들깻잎, 부추, 마늘쫑, 마늘, 생강, 표고버섯) 304건을 대상으로 bifenazate의 잔류량을 조사한 결과 모든 농산물에서 검출되지 않았다.