[화학] 전기화학적 방법에 의한 효소의 최적 pH 결정
A biosensor for the determination of hydrogen peroxide was constructed by immobilizing of porcine small instestinal tissue in a plain carbon paste, and the effect of varying the $H_2O_2$ concentration and pH on the rate of catalytic reaction was evaluated. For the mathematical simplicity, no mediato...
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| Veröffentlicht in: | Punsŏk kwahak 2000, Vol.13 (1), p.41-48 |
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| creator | 윤길중(Kil-Joong Yoon) 이범규(Beom-Gyu Lee) 권효식(Hyo-Shik Kwon) |
| description | A biosensor for the determination of hydrogen peroxide was constructed by immobilizing of porcine small instestinal tissue in a plain carbon paste, and the effect of varying the $H_2O_2$ concentration and pH on the rate of catalytic reaction was evaluated. For the mathematical simplicity, no mediator was added. Electrochemical properties and the maximal rate could be derived from the quantitative analysis of the observed phenomena related to the electrode reaction. Also, pH dependence of the Michaelis constant enabled to calculate various thermodynamic parameters and subsequently to design a electrochemical method to determine the optimum pH of enzyme. 돼지의 소장조직을 탄소반죽에 혼입시켜 과산화수소 정량 바이오센서를 제작하고, 기질농도와 pH변화가 전극의 효소반응속도에 미치는 영향을 관찰하였다. 수학적 취급이 용이하도록 매개체는 첨가하지 않았다. 관찰된 현상들을 정량적으로 처리하여 전극반응에 관련된 효소의 전기화학적 특성과 최대 반응속도를 구하였다. 또, pH에 따른 Michaelis 상수의 변화로부터 여러 가지 열역학적 파라미터를 얻고, 이를 이용하여 효소활동의 최적 pH를 구하는 방법을 고안하였다. |
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For the mathematical simplicity, no mediator was added. Electrochemical properties and the maximal rate could be derived from the quantitative analysis of the observed phenomena related to the electrode reaction. Also, pH dependence of the Michaelis constant enabled to calculate various thermodynamic parameters and subsequently to design a electrochemical method to determine the optimum pH of enzyme. 돼지의 소장조직을 탄소반죽에 혼입시켜 과산화수소 정량 바이오센서를 제작하고, 기질농도와 pH변화가 전극의 효소반응속도에 미치는 영향을 관찰하였다. 수학적 취급이 용이하도록 매개체는 첨가하지 않았다. 관찰된 현상들을 정량적으로 처리하여 전극반응에 관련된 효소의 전기화학적 특성과 최대 반응속도를 구하였다. 또, pH에 따른 Michaelis 상수의 변화로부터 여러 가지 열역학적 파라미터를 얻고, 이를 이용하여 효소활동의 최적 pH를 구하는 방법을 고안하였다.</description><identifier>ISSN: 1225-0163</identifier><identifier>EISSN: 2288-8985</identifier><language>kor</language><publisher>한국분석과학회</publisher><ispartof>Punsŏk kwahak, 2000, Vol.13 (1), p.41-48</ispartof><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,4024</link.rule.ids></links><search><creatorcontrib>윤길중(Kil-Joong Yoon)</creatorcontrib><creatorcontrib>이범규(Beom-Gyu Lee)</creatorcontrib><creatorcontrib>권효식(Hyo-Shik Kwon)</creatorcontrib><title>[화학] 전기화학적 방법에 의한 효소의 최적 pH 결정</title><title>Punsŏk kwahak</title><addtitle>Analytical science & technology</addtitle><description>A biosensor for the determination of hydrogen peroxide was constructed by immobilizing of porcine small instestinal tissue in a plain carbon paste, and the effect of varying the $H_2O_2$ concentration and pH on the rate of catalytic reaction was evaluated. For the mathematical simplicity, no mediator was added. Electrochemical properties and the maximal rate could be derived from the quantitative analysis of the observed phenomena related to the electrode reaction. Also, pH dependence of the Michaelis constant enabled to calculate various thermodynamic parameters and subsequently to design a electrochemical method to determine the optimum pH of enzyme. 돼지의 소장조직을 탄소반죽에 혼입시켜 과산화수소 정량 바이오센서를 제작하고, 기질농도와 pH변화가 전극의 효소반응속도에 미치는 영향을 관찰하였다. 수학적 취급이 용이하도록 매개체는 첨가하지 않았다. 관찰된 현상들을 정량적으로 처리하여 전극반응에 관련된 효소의 전기화학적 특성과 최대 반응속도를 구하였다. 또, pH에 따른 Michaelis 상수의 변화로부터 여러 가지 열역학적 파라미터를 얻고, 이를 이용하여 효소활동의 최적 pH를 구하는 방법을 고안하였다.</description><issn>1225-0163</issn><issn>2288-8985</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>JDI</sourceid><recordid>eNpFj79Lw0AcxQ9RsNT-D1kcA_cjd_neWNpq1WKWbiLh0utBbC2l0cFRECcHB0OtVMioWygI_k3t5X_wpIJvee_BhwdvB9UoBfBBAt9FNUIp9zERbB81suwKO_EgZFLUUPuiWrxU-eLSs8XD-rvcNlvce5vyc7PK7fzZs--vVb70qrcP-_jkime_lr_EtOutV6Ut8gO0Z9Q4Gzb-vI76R51-q-v3ouOTVrPnj4AKX0AoMVGgFceJSozRJgSqMMUhVwqwINpZoo1JBoSrhEg94AQMC4DzQAesjg63s6M0u0njic7G8WnzLKLuD5FYAPAAJP7nJrez9HqoUxVPXVCzu_g8ancwFow7nP0AUiplVg</recordid><startdate>2000</startdate><enddate>2000</enddate><creator>윤길중(Kil-Joong Yoon)</creator><creator>이범규(Beom-Gyu Lee)</creator><creator>권효식(Hyo-Shik Kwon)</creator><general>한국분석과학회</general><scope>DBRKI</scope><scope>TDB</scope><scope>JDI</scope></search><sort><creationdate>2000</creationdate><title>[화학] 전기화학적 방법에 의한 효소의 최적 pH 결정</title><author>윤길중(Kil-Joong Yoon) ; 이범규(Beom-Gyu Lee) ; 권효식(Hyo-Shik Kwon)</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-k826-687901a8da50babffdf782a02075aa8061daa8bdffbc15ab19dc518f348554d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>kor</language><creationdate>2000</creationdate><toplevel>online_resources</toplevel><creatorcontrib>윤길중(Kil-Joong Yoon)</creatorcontrib><creatorcontrib>이범규(Beom-Gyu Lee)</creatorcontrib><creatorcontrib>권효식(Hyo-Shik Kwon)</creatorcontrib><collection>DBPIA - 디비피아</collection><collection>DBPIA</collection><collection>KoreaScience</collection><jtitle>Punsŏk kwahak</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>윤길중(Kil-Joong Yoon)</au><au>이범규(Beom-Gyu Lee)</au><au>권효식(Hyo-Shik Kwon)</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>[화학] 전기화학적 방법에 의한 효소의 최적 pH 결정</atitle><jtitle>Punsŏk kwahak</jtitle><addtitle>Analytical science & technology</addtitle><date>2000</date><risdate>2000</risdate><volume>13</volume><issue>1</issue><spage>41</spage><epage>48</epage><pages>41-48</pages><issn>1225-0163</issn><eissn>2288-8985</eissn><abstract>A biosensor for the determination of hydrogen peroxide was constructed by immobilizing of porcine small instestinal tissue in a plain carbon paste, and the effect of varying the $H_2O_2$ concentration and pH on the rate of catalytic reaction was evaluated. For the mathematical simplicity, no mediator was added. Electrochemical properties and the maximal rate could be derived from the quantitative analysis of the observed phenomena related to the electrode reaction. Also, pH dependence of the Michaelis constant enabled to calculate various thermodynamic parameters and subsequently to design a electrochemical method to determine the optimum pH of enzyme. 돼지의 소장조직을 탄소반죽에 혼입시켜 과산화수소 정량 바이오센서를 제작하고, 기질농도와 pH변화가 전극의 효소반응속도에 미치는 영향을 관찰하였다. 수학적 취급이 용이하도록 매개체는 첨가하지 않았다. 관찰된 현상들을 정량적으로 처리하여 전극반응에 관련된 효소의 전기화학적 특성과 최대 반응속도를 구하였다. 또, pH에 따른 Michaelis 상수의 변화로부터 여러 가지 열역학적 파라미터를 얻고, 이를 이용하여 효소활동의 최적 pH를 구하는 방법을 고안하였다.</abstract><pub>한국분석과학회</pub><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| title | [화학] 전기화학적 방법에 의한 효소의 최적 pH 결정 |
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