사과 겹무늬썩음병에 걸린 가지로부터 분산되는 병포자의 정량적 조사법 개발

On the basis of the fact that the pycnidiospore of Botryosphaeria dothidea, the causal fungus of apple white rot is a typical water borne spore, a method for quantitative analysis of pycnidiospore dispersal from the warts produced on the diseased apple tree stem was developed. The warts on which cra...

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Veröffentlicht in:한국식물병리학회지 1998, Vol.14 (4), p.325-330
Hauptverfasser: 양희정, 최창희, 우현, 김대희, 엄재열, Yang, Hee-Jung, Choi, Chang-Hee, Woo, Hyun, Kim, Dai-Hee, Uhm, Jae-Youl
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container_end_page 330
container_issue 4
container_start_page 325
container_title 한국식물병리학회지
container_volume 14
creator 양희정
최창희
우현
김대희
엄재열
Yang, Hee-Jung
Choi, Chang-Hee
Woo, Hyun
Kim, Dai-Hee
Uhm, Jae-Youl
description On the basis of the fact that the pycnidiospore of Botryosphaeria dothidea, the causal fungus of apple white rot is a typical water borne spore, a method for quantitative analysis of pycnidiospore dispersal from the warts produced on the diseased apple tree stem was developed. The warts on which cracks developed either on or around them were cut off at the base, and shaked in the water for 4hours at 2$0^{\circ}C$, in which condition the maximum number of spores were released. The volume of shaking solution was calculated as 1 ml per one wart. At the end of shaking, Trio, a household detergent was added to the shaking solution to the concentration of 0.1%, and shaked for additional 10 minutes at 35$^{\circ}C$ to take off the spores attached on the glass ware. One milliliter of the spore suspension thus prepared were passed through transparent membrane filter (pore size : 3.0 ${\mu}{\textrm}{m}$), and the spores attached on the filter were counted under a microscope ($\times$200) after staining them with lactophenol supplemented with aniline blue. The results thus obtained were statistically consistent when at least 30 warts were used simultaneously in single shaking. This method can be applicable in the elucidation of ecology of sporulation and spore dispersal, and also in the screening of the sporulation inhibitor which can be used in the control of the disease by reducing the inoculum density.
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The warts on which cracks developed either on or around them were cut off at the base, and shaked in the water for 4hours at 2$0^{\circ}C$, in which condition the maximum number of spores were released. The volume of shaking solution was calculated as 1 ml per one wart. At the end of shaking, Trio, a household detergent was added to the shaking solution to the concentration of 0.1%, and shaked for additional 10 minutes at 35$^{\circ}C$ to take off the spores attached on the glass ware. One milliliter of the spore suspension thus prepared were passed through transparent membrane filter (pore size : 3.0 ${\mu}{\textrm}{m}$), and the spores attached on the filter were counted under a microscope ($\times$200) after staining them with lactophenol supplemented with aniline blue. The results thus obtained were statistically consistent when at least 30 warts were used simultaneously in single shaking. 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This method can be applicable in the elucidation of ecology of sporulation and spore dispersal, and also in the screening of the sporulation inhibitor which can be used in the control of the disease by reducing the inoculum density.</abstract><oa>free_for_read</oa></addata></record>
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title 사과 겹무늬썩음병에 걸린 가지로부터 분산되는 병포자의 정량적 조사법 개발
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