[약학] 간조직 센서를 이용한 dopamine의 정량
A tissue biosensor for the measurement of dopamine has been constructed by immobilizing the slice of Sprague-Dawley rat liver on $NH_3$-sensing electrode. To overcome the defect of tissue sensors, the maximal velocity of response curve was measured and applied to the Lineweaver-Burk equation instead...
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| Veröffentlicht in: | Punsŏk kwahak 1995, Vol.8 (3), p.365-370 |
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| creator | 허문희(Moon-Hye Hur) 김기묘(Ki-Myo Kim) 김영학(Young-Hak Kim) 백광진(Kwang-Jin Paek) 최현영(Hyun-Young Choi) 안문규(Moon-Kyu Ahn) |
| description | A tissue biosensor for the measurement of dopamine has been constructed by immobilizing the slice of Sprague-Dawley rat liver on $NH_3$-sensing electrode. To overcome the defect of tissue sensors, the maximal velocity of response curve was measured and applied to the Lineweaver-Burk equation instead of the Nernst equation. And then compared the results with those obtained from Nernst equation. When we obtained calibration curves from Nernst equation, there were variances on the slope and the linear range. But from Lineweaver-Burk equation, the scale of variance was small. Response time was reduced from 7~12 minutes to 2~3 minutes. Dopamine 정량을 위한 조직 센서로 Sprague-Dawley계 숫쥐의 간조직을 암모니아 기체 감응 전극에 고정화하였다. 조직 센서가 가지고 있는 단점을 극복하기 위해 기존의 Nernst equation 대신, 감응 곡선의 최대 속도를 측정하여 Lineweauer-Burk equation에 적용함으로써 그 가능성을 살펴보았다. 기존의 방법으로는 조직마다 slope, linear range에 변화가 심하나 본 방법에 따른 경우 그 변화 정도가 적으며, 감응 시간은 7분~12분 정도로부터 2~3분으로 단축시킬 수 있었다. |
| format | Article |
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To overcome the defect of tissue sensors, the maximal velocity of response curve was measured and applied to the Lineweaver-Burk equation instead of the Nernst equation. And then compared the results with those obtained from Nernst equation. When we obtained calibration curves from Nernst equation, there were variances on the slope and the linear range. But from Lineweaver-Burk equation, the scale of variance was small. Response time was reduced from 7~12 minutes to 2~3 minutes. Dopamine 정량을 위한 조직 센서로 Sprague-Dawley계 숫쥐의 간조직을 암모니아 기체 감응 전극에 고정화하였다. 조직 센서가 가지고 있는 단점을 극복하기 위해 기존의 Nernst equation 대신, 감응 곡선의 최대 속도를 측정하여 Lineweauer-Burk equation에 적용함으로써 그 가능성을 살펴보았다. 기존의 방법으로는 조직마다 slope, linear range에 변화가 심하나 본 방법에 따른 경우 그 변화 정도가 적으며, 감응 시간은 7분~12분 정도로부터 2~3분으로 단축시킬 수 있었다.</description><identifier>ISSN: 1225-0163</identifier><identifier>EISSN: 2288-8985</identifier><language>kor</language><publisher>한국분석과학회</publisher><ispartof>Punsŏk kwahak, 1995, Vol.8 (3), p.365-370</ispartof><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,4009</link.rule.ids></links><search><creatorcontrib>허문희(Moon-Hye Hur)</creatorcontrib><creatorcontrib>김기묘(Ki-Myo Kim)</creatorcontrib><creatorcontrib>김영학(Young-Hak Kim)</creatorcontrib><creatorcontrib>백광진(Kwang-Jin Paek)</creatorcontrib><creatorcontrib>최현영(Hyun-Young Choi)</creatorcontrib><creatorcontrib>안문규(Moon-Kyu Ahn)</creatorcontrib><title>[약학] 간조직 센서를 이용한 dopamine의 정량</title><title>Punsŏk kwahak</title><addtitle>Analytical science & technology</addtitle><description>A tissue biosensor for the measurement of dopamine has been constructed by immobilizing the slice of Sprague-Dawley rat liver on $NH_3$-sensing electrode. To overcome the defect of tissue sensors, the maximal velocity of response curve was measured and applied to the Lineweaver-Burk equation instead of the Nernst equation. And then compared the results with those obtained from Nernst equation. When we obtained calibration curves from Nernst equation, there were variances on the slope and the linear range. But from Lineweaver-Burk equation, the scale of variance was small. Response time was reduced from 7~12 minutes to 2~3 minutes. Dopamine 정량을 위한 조직 센서로 Sprague-Dawley계 숫쥐의 간조직을 암모니아 기체 감응 전극에 고정화하였다. 조직 센서가 가지고 있는 단점을 극복하기 위해 기존의 Nernst equation 대신, 감응 곡선의 최대 속도를 측정하여 Lineweauer-Burk equation에 적용함으로써 그 가능성을 살펴보았다. 기존의 방법으로는 조직마다 slope, linear range에 변화가 심하나 본 방법에 따른 경우 그 변화 정도가 적으며, 감응 시간은 7분~12분 정도로부터 2~3분으로 단축시킬 수 있었다.</description><issn>1225-0163</issn><issn>2288-8985</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>JDI</sourceid><recordid>eNpFjMtKw0AYhQdRMNS-QzYuA3PpTP5ZllqvxWy6EwmT_BOIbWNpdOFS6MYHCFhpURci7rpwWV_ITt_BgIJncw6Hj2-LeJwDBKBBbhOPcS4DypTYJc2yvKJ1ZCsUWnlEX7jqa1PNLv3v5dS9Lt37ve-mKzedr99Wvlt8uqePTTX38XpsRnlh3eLRdy_V-vlhj-xkZlja5l83SP-w2-8cB73o6KTT7gUD4CqwFiDjoCWjRoc0TS0C46p-E6Qi0ylNUhZiS3PDUKWIEmWSoMxCmSVWWNEg-7_aQV7e5HGB5TA-bZ9FTNdOTRWAVKD5P1fcTvKRxdzE43qYyV18Hh10KVVC1qj4AXHhXcA</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>허문희(Moon-Hye Hur)</creator><creator>김기묘(Ki-Myo Kim)</creator><creator>김영학(Young-Hak Kim)</creator><creator>백광진(Kwang-Jin Paek)</creator><creator>최현영(Hyun-Young Choi)</creator><creator>안문규(Moon-Kyu Ahn)</creator><general>한국분석과학회</general><scope>DBRKI</scope><scope>TDB</scope><scope>JDI</scope></search><sort><creationdate>1995</creationdate><title>[약학] 간조직 센서를 이용한 dopamine의 정량</title><author>허문희(Moon-Hye Hur) ; 김기묘(Ki-Myo Kim) ; 김영학(Young-Hak Kim) ; 백광진(Kwang-Jin Paek) ; 최현영(Hyun-Young Choi) ; 안문규(Moon-Kyu Ahn)</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-k826-ee88f289510a970cced8126e88bd03f9c0bc17d492a1d6cdd5d5bbd5f75fbe3e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>kor</language><creationdate>1995</creationdate><toplevel>online_resources</toplevel><creatorcontrib>허문희(Moon-Hye Hur)</creatorcontrib><creatorcontrib>김기묘(Ki-Myo Kim)</creatorcontrib><creatorcontrib>김영학(Young-Hak Kim)</creatorcontrib><creatorcontrib>백광진(Kwang-Jin Paek)</creatorcontrib><creatorcontrib>최현영(Hyun-Young Choi)</creatorcontrib><creatorcontrib>안문규(Moon-Kyu Ahn)</creatorcontrib><collection>DBPIA - 디비피아</collection><collection>DBPIA</collection><collection>KoreaScience</collection><jtitle>Punsŏk kwahak</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>허문희(Moon-Hye Hur)</au><au>김기묘(Ki-Myo Kim)</au><au>김영학(Young-Hak Kim)</au><au>백광진(Kwang-Jin Paek)</au><au>최현영(Hyun-Young Choi)</au><au>안문규(Moon-Kyu Ahn)</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>[약학] 간조직 센서를 이용한 dopamine의 정량</atitle><jtitle>Punsŏk kwahak</jtitle><addtitle>Analytical science & technology</addtitle><date>1995</date><risdate>1995</risdate><volume>8</volume><issue>3</issue><spage>365</spage><epage>370</epage><pages>365-370</pages><issn>1225-0163</issn><eissn>2288-8985</eissn><abstract>A tissue biosensor for the measurement of dopamine has been constructed by immobilizing the slice of Sprague-Dawley rat liver on $NH_3$-sensing electrode. To overcome the defect of tissue sensors, the maximal velocity of response curve was measured and applied to the Lineweaver-Burk equation instead of the Nernst equation. And then compared the results with those obtained from Nernst equation. When we obtained calibration curves from Nernst equation, there were variances on the slope and the linear range. But from Lineweaver-Burk equation, the scale of variance was small. Response time was reduced from 7~12 minutes to 2~3 minutes. Dopamine 정량을 위한 조직 센서로 Sprague-Dawley계 숫쥐의 간조직을 암모니아 기체 감응 전극에 고정화하였다. 조직 센서가 가지고 있는 단점을 극복하기 위해 기존의 Nernst equation 대신, 감응 곡선의 최대 속도를 측정하여 Lineweauer-Burk equation에 적용함으로써 그 가능성을 살펴보았다. 기존의 방법으로는 조직마다 slope, linear range에 변화가 심하나 본 방법에 따른 경우 그 변화 정도가 적으며, 감응 시간은 7분~12분 정도로부터 2~3분으로 단축시킬 수 있었다.</abstract><pub>한국분석과학회</pub><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| title | [약학] 간조직 센서를 이용한 dopamine의 정량 |
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