Effects and Mechanism of AP39 on Ovarian Functions in Rats Exposed to Cisplatin and Chronic Immobilization Stress
Objectives: Premature ovarian failure (POF) rat models are essential for elucidating the hormonal and ovarian molecular mechanisms of human POF diseases and developing new therapeutic agents. This study aimed to compare the applicability of chronic immobilization stress (CIS) as a POF model with tha...
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Veröffentlicht in: | Journal of menopausal medicine 2024-08, Vol.30 (2), p.104 |
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creator | Ebru Onalan Bilgi Erbay İlay Kavuran Buran Deniz Erol Ahmet Tektemur Tuncay Kuloglu Ibrahim Hanifi Ozercan |
description | Objectives: Premature ovarian failure (POF) rat models are essential for elucidating the hormonal and ovarian molecular mechanisms of human POF diseases and developing new therapeutic agents. This study aimed to compare the applicability of chronic immobilization stress (CIS) as a POF model with that of cisplatin and to examine the impact of AP39, a mitochondrial protective agent, on ovarian function in rats treated with cisplatin and CIS.
Methods: Sixty Sprague-Dawley female rats were divided equally into six groups (10 per group): Control, Cisplatin, AP39, Cisplatin + AP39, CIS, and CIS + AP39. Ovarian dysfunction was induced with cisplatin (3 mg/kg) or CIS. Forced swim test, hormone concentrations, estrous cyclicity, histopathology, follicle counts, and molecular alterations in the ovary and mitochondria were analyzed.
Results: In the CIS and cisplatin groups, mitochondrial biogenesis, egg quality, hormonal profile, estrous cycle, and folliculogenesis significantly declined. Nonetheless, most of the parameters with undesirable results did not normalize after AP39 administration.
Conclusions: The cisplatin- and CIS-treated rats exhibited unshared deteriorated hormonal pathways and similarly disrupted gene expression patterns. Our current CIS model did not meet the human POF criteria, which include decreased estradiol levels, despite having advantages in terms of ease of modeling and reproducibility and demonstrating pathological changes similar to those observed in human POF. Therefore, rather than using this model as an POF model, using it as a representation of stress-induced ovarian dysfunction would be more appropriate. |
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Methods: Sixty Sprague-Dawley female rats were divided equally into six groups (10 per group): Control, Cisplatin, AP39, Cisplatin + AP39, CIS, and CIS + AP39. Ovarian dysfunction was induced with cisplatin (3 mg/kg) or CIS. Forced swim test, hormone concentrations, estrous cyclicity, histopathology, follicle counts, and molecular alterations in the ovary and mitochondria were analyzed.
Results: In the CIS and cisplatin groups, mitochondrial biogenesis, egg quality, hormonal profile, estrous cycle, and folliculogenesis significantly declined. Nonetheless, most of the parameters with undesirable results did not normalize after AP39 administration.
Conclusions: The cisplatin- and CIS-treated rats exhibited unshared deteriorated hormonal pathways and similarly disrupted gene expression patterns. Our current CIS model did not meet the human POF criteria, which include decreased estradiol levels, despite having advantages in terms of ease of modeling and reproducibility and demonstrating pathological changes similar to those observed in human POF. Therefore, rather than using this model as an POF model, using it as a representation of stress-induced ovarian dysfunction would be more appropriate.</description><identifier>ISSN: 2288-6478</identifier><language>kor</language><publisher>대한폐경학회</publisher><subject>AP39 ; Chronic immobilization stress ; Cisplatin ; Premature ovarian failure</subject><ispartof>Journal of menopausal medicine, 2024-08, Vol.30 (2), p.104</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Ebru Onalan</creatorcontrib><creatorcontrib>Bilgi Erbay</creatorcontrib><creatorcontrib>İlay Kavuran Buran</creatorcontrib><creatorcontrib>Deniz Erol</creatorcontrib><creatorcontrib>Ahmet Tektemur</creatorcontrib><creatorcontrib>Tuncay Kuloglu</creatorcontrib><creatorcontrib>Ibrahim Hanifi Ozercan</creatorcontrib><title>Effects and Mechanism of AP39 on Ovarian Functions in Rats Exposed to Cisplatin and Chronic Immobilization Stress</title><title>Journal of menopausal medicine</title><addtitle>대한폐경학회지</addtitle><description>Objectives: Premature ovarian failure (POF) rat models are essential for elucidating the hormonal and ovarian molecular mechanisms of human POF diseases and developing new therapeutic agents. This study aimed to compare the applicability of chronic immobilization stress (CIS) as a POF model with that of cisplatin and to examine the impact of AP39, a mitochondrial protective agent, on ovarian function in rats treated with cisplatin and CIS.
Methods: Sixty Sprague-Dawley female rats were divided equally into six groups (10 per group): Control, Cisplatin, AP39, Cisplatin + AP39, CIS, and CIS + AP39. Ovarian dysfunction was induced with cisplatin (3 mg/kg) or CIS. Forced swim test, hormone concentrations, estrous cyclicity, histopathology, follicle counts, and molecular alterations in the ovary and mitochondria were analyzed.
Results: In the CIS and cisplatin groups, mitochondrial biogenesis, egg quality, hormonal profile, estrous cycle, and folliculogenesis significantly declined. Nonetheless, most of the parameters with undesirable results did not normalize after AP39 administration.
Conclusions: The cisplatin- and CIS-treated rats exhibited unshared deteriorated hormonal pathways and similarly disrupted gene expression patterns. Our current CIS model did not meet the human POF criteria, which include decreased estradiol levels, despite having advantages in terms of ease of modeling and reproducibility and demonstrating pathological changes similar to those observed in human POF. Therefore, rather than using this model as an POF model, using it as a representation of stress-induced ovarian dysfunction would be more appropriate.</description><subject>AP39</subject><subject>Chronic immobilization stress</subject><subject>Cisplatin</subject><subject>Premature ovarian failure</subject><issn>2288-6478</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9jMEKgkAUAPdQUFRf0OX9QJC2qR1DlDpEUd3jqSs90l3bt0X19Sl07jSHYaYnhr4fRbNAhtFATJgpm0sZyuU8CIfinpSlyh0D6gJ2Kr-iJq7BlLA-LFZgNOyfaAk1pA-dOzKagTQcsU2SV2NYFeAMxMRNha413Se-WqMph21dm4wq-mAXwslZxTwW_RIrVpMfR2KaJud4M7sR86WxVKN9X6TnBb4fLP7bL_ARRLE</recordid><startdate>20240831</startdate><enddate>20240831</enddate><creator>Ebru Onalan</creator><creator>Bilgi Erbay</creator><creator>İlay Kavuran Buran</creator><creator>Deniz Erol</creator><creator>Ahmet Tektemur</creator><creator>Tuncay Kuloglu</creator><creator>Ibrahim Hanifi Ozercan</creator><general>대한폐경학회</general><scope>HZB</scope><scope>Q5X</scope></search><sort><creationdate>20240831</creationdate><title>Effects and Mechanism of AP39 on Ovarian Functions in Rats Exposed to Cisplatin and Chronic Immobilization Stress</title><author>Ebru Onalan ; Bilgi Erbay ; İlay Kavuran Buran ; Deniz Erol ; Ahmet Tektemur ; Tuncay Kuloglu ; Ibrahim Hanifi Ozercan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-kiss_primary_41162263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>kor</language><creationdate>2024</creationdate><topic>AP39</topic><topic>Chronic immobilization stress</topic><topic>Cisplatin</topic><topic>Premature ovarian failure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ebru Onalan</creatorcontrib><creatorcontrib>Bilgi Erbay</creatorcontrib><creatorcontrib>İlay Kavuran Buran</creatorcontrib><creatorcontrib>Deniz Erol</creatorcontrib><creatorcontrib>Ahmet Tektemur</creatorcontrib><creatorcontrib>Tuncay Kuloglu</creatorcontrib><creatorcontrib>Ibrahim Hanifi Ozercan</creatorcontrib><collection>Korean Studies Information Service System (KISS)</collection><collection>Korean Studies Information Service System (KISS) B-Type</collection><jtitle>Journal of menopausal medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ebru Onalan</au><au>Bilgi Erbay</au><au>İlay Kavuran Buran</au><au>Deniz Erol</au><au>Ahmet Tektemur</au><au>Tuncay Kuloglu</au><au>Ibrahim Hanifi Ozercan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects and Mechanism of AP39 on Ovarian Functions in Rats Exposed to Cisplatin and Chronic Immobilization Stress</atitle><jtitle>Journal of menopausal medicine</jtitle><addtitle>대한폐경학회지</addtitle><date>2024-08-31</date><risdate>2024</risdate><volume>30</volume><issue>2</issue><spage>104</spage><pages>104-</pages><issn>2288-6478</issn><abstract>Objectives: Premature ovarian failure (POF) rat models are essential for elucidating the hormonal and ovarian molecular mechanisms of human POF diseases and developing new therapeutic agents. This study aimed to compare the applicability of chronic immobilization stress (CIS) as a POF model with that of cisplatin and to examine the impact of AP39, a mitochondrial protective agent, on ovarian function in rats treated with cisplatin and CIS.
Methods: Sixty Sprague-Dawley female rats were divided equally into six groups (10 per group): Control, Cisplatin, AP39, Cisplatin + AP39, CIS, and CIS + AP39. Ovarian dysfunction was induced with cisplatin (3 mg/kg) or CIS. Forced swim test, hormone concentrations, estrous cyclicity, histopathology, follicle counts, and molecular alterations in the ovary and mitochondria were analyzed.
Results: In the CIS and cisplatin groups, mitochondrial biogenesis, egg quality, hormonal profile, estrous cycle, and folliculogenesis significantly declined. Nonetheless, most of the parameters with undesirable results did not normalize after AP39 administration.
Conclusions: The cisplatin- and CIS-treated rats exhibited unshared deteriorated hormonal pathways and similarly disrupted gene expression patterns. Our current CIS model did not meet the human POF criteria, which include decreased estradiol levels, despite having advantages in terms of ease of modeling and reproducibility and demonstrating pathological changes similar to those observed in human POF. Therefore, rather than using this model as an POF model, using it as a representation of stress-induced ovarian dysfunction would be more appropriate.</abstract><pub>대한폐경학회</pub><tpages>16</tpages></addata></record> |
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source | DOAJ Directory of Open Access Journals; PubMed Central Open Access; KoreaMed Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
subjects | AP39 Chronic immobilization stress Cisplatin Premature ovarian failure |
title | Effects and Mechanism of AP39 on Ovarian Functions in Rats Exposed to Cisplatin and Chronic Immobilization Stress |
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