Purification, Structural Characterization, and Biological Activity of Polysaccharides from Lentinus velutinus
A polysaccharide (LVP) was purified from fruiting body of Lentinus velutinus by ethanol precipitation fractionation and DEAE and Sephadex G-100 column chromatography. The yield of purified polysaccharide was 0.025%. Molecular characteristics of LVP were determined by gel permeation chromatography, F...
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| Veröffentlicht in: | Mycobiology 2020-02, Vol.48 (1), p.52 |
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| creator | Wascharin Udchumpisai Eakaphun Bangyeekhun |
| description | A polysaccharide (LVP) was purified from fruiting body of Lentinus velutinus by ethanol precipitation fractionation and DEAE and Sephadex G-100 column chromatography. The yield of purified polysaccharide was 0.025%. Molecular characteristics of LVP were determined by gel permeation chromatography, FT-IR spectroscopy, and thin-layer chromatography. Our results revealed that LVP is a polysaccharide composed of only glucose units, and has a molecular weight of 336 kDa. Biological activity assays indicated that LVP exhibits both cytotoxic and antioxidant activity. LVP showed specific cytotoxicity against cancer cells (HeLa and HepG2 cells), and alterations in cancer cell morphology were found after LVP treatment. |
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The yield of purified polysaccharide was 0.025%. Molecular characteristics of LVP were determined by gel permeation chromatography, FT-IR spectroscopy, and thin-layer chromatography. Our results revealed that LVP is a polysaccharide composed of only glucose units, and has a molecular weight of 336 kDa. Biological activity assays indicated that LVP exhibits both cytotoxic and antioxidant activity. LVP showed specific cytotoxicity against cancer cells (HeLa and HepG2 cells), and alterations in cancer cell morphology were found after LVP treatment.</description><identifier>ISSN: 1229-8093</identifier><language>kor</language><publisher>한국균학회</publisher><subject>antioxidant activity ; cytotoxicity ; Lentinus velutinus ; polysaccharides</subject><ispartof>Mycobiology, 2020-02, Vol.48 (1), p.52</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Wascharin Udchumpisai</creatorcontrib><creatorcontrib>Eakaphun Bangyeekhun</creatorcontrib><title>Purification, Structural Characterization, and Biological Activity of Polysaccharides from Lentinus velutinus</title><title>Mycobiology</title><addtitle>Mycobiology</addtitle><description>A polysaccharide (LVP) was purified from fruiting body of Lentinus velutinus by ethanol precipitation fractionation and DEAE and Sephadex G-100 column chromatography. 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LVP showed specific cytotoxicity against cancer cells (HeLa and HepG2 cells), and alterations in cancer cell morphology were found after LVP treatment.</description><subject>antioxidant activity</subject><subject>cytotoxicity</subject><subject>Lentinus velutinus</subject><subject>polysaccharides</subject><issn>1229-8093</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9jssKgkAYhWdRkJRP0OZ_gARz8rYsKVq0EGov0zjWT6MTcxHs6ZNw3eoc-L4DZ0a8bRTlQRbmdEF8Y_AexpSmSbZLPdKWTmODnFlU3QauVjtunWYSiifTjFuh8TNB1tVwQCXVY_Ql7LnFHu0AqoFSycEwzscN1sJAo1ULF9FZ7JyBXkj3aysyb5g0wp9ySdan4604By80pnprbJkeqvFbEkc5_U-_Fj9Flw</recordid><startdate>20200228</startdate><enddate>20200228</enddate><creator>Wascharin Udchumpisai</creator><creator>Eakaphun Bangyeekhun</creator><general>한국균학회</general><scope>HZB</scope><scope>Q5X</scope></search><sort><creationdate>20200228</creationdate><title>Purification, Structural Characterization, and Biological Activity of Polysaccharides from Lentinus velutinus</title><author>Wascharin Udchumpisai ; Eakaphun Bangyeekhun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-kiss_primary_37665293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>kor</language><creationdate>2020</creationdate><topic>antioxidant activity</topic><topic>cytotoxicity</topic><topic>Lentinus velutinus</topic><topic>polysaccharides</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wascharin Udchumpisai</creatorcontrib><creatorcontrib>Eakaphun Bangyeekhun</creatorcontrib><collection>Korea Information Science Society (KISS)</collection><collection>Korean Studies Information Service System (KISS) B-Type</collection><jtitle>Mycobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wascharin Udchumpisai</au><au>Eakaphun Bangyeekhun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification, Structural Characterization, and Biological Activity of Polysaccharides from Lentinus velutinus</atitle><jtitle>Mycobiology</jtitle><addtitle>Mycobiology</addtitle><date>2020-02-28</date><risdate>2020</risdate><volume>48</volume><issue>1</issue><spage>52</spage><pages>52-</pages><issn>1229-8093</issn><abstract>A polysaccharide (LVP) was purified from fruiting body of Lentinus velutinus by ethanol precipitation fractionation and DEAE and Sephadex G-100 column chromatography. The yield of purified polysaccharide was 0.025%. Molecular characteristics of LVP were determined by gel permeation chromatography, FT-IR spectroscopy, and thin-layer chromatography. Our results revealed that LVP is a polysaccharide composed of only glucose units, and has a molecular weight of 336 kDa. Biological activity assays indicated that LVP exhibits both cytotoxic and antioxidant activity. LVP showed specific cytotoxicity against cancer cells (HeLa and HepG2 cells), and alterations in cancer cell morphology were found after LVP treatment.</abstract><pub>한국균학회</pub><tpages>8</tpages></addata></record> |
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| source | DOAJ Directory of Open Access Journals; PubMed Central Open Access; KoreaMed Open Access; Access via Taylor & Francis (Open Access Collection); EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | antioxidant activity cytotoxicity Lentinus velutinus polysaccharides |
| title | Purification, Structural Characterization, and Biological Activity of Polysaccharides from Lentinus velutinus |
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