BMB Reports : Loss of phospholipase D2 impairs VEGF-induced angiogenesis

Vascular endothelial growth factor (VEGF) is a key mediator of angiogenesis and critical for normal embryonic development and repair of pathophysiological conditions in adults. Although phospholipase D (PLD) activity has been implicated in angiogenic processes, its role in VEGF signaling during angi...

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Veröffentlicht in:	BMB reports 2016-03, Vol.49 (3), p.191
Hauptverfasser:	Chang Sup Lee, Jaewang Ghim, Parkyong Song, Pann Ghill Suh, Sung Ho Ryu
Format:	Artikel
Sprache:	kor
Schlagworte:	Angiogenesis Aorta ring Endothelial cells Phospholipase D Tube formation VEGF
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creator	Chang Sup Lee Jaewang Ghim Parkyong Song Pann Ghill Suh Sung Ho Ryu
description	Vascular endothelial growth factor (VEGF) is a key mediator of angiogenesis and critical for normal embryonic development and repair of pathophysiological conditions in adults. Although phospholipase D (PLD) activity has been implicated in angiogenic processes, its role in VEGF signaling during angiogenesis in mammals is unclear. Here, we found that silencing of PLD2 by siRNA blocked VEGF-mediated signaling in immortalized human umbilical vein endothelial cells (iHUVECs). Also, VEGF-induced endothelial cell survival, proliferation, migration, and tube formation were inhibited by PLD2 silencing. Furthermore, while Pld2-knockout mice exhibited normal development, loss of PLD2 inhibited VEGF-mediated ex vivo angiogenesis. These findings suggest that PLD2 functions as a key mediator in the VEGF-mediated angiogenic functions of endothelial cells. [BMB Reports 2016; 49(3): 191-196]
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source	PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry
subjects	Angiogenesis Aorta ring Endothelial cells Phospholipase D Tube formation VEGF
title	BMB Reports : Loss of phospholipase D2 impairs VEGF-induced angiogenesis
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